The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing
Placenta accreta spectrum disorders (PAS) are severe pregnancy complications that occur when extravillous trophoblast cells (EVTs) invade beyond the uterine inner myometrium and are characterized by hypervascularity on prenatal ultrasound and catastrophic postpartum hemorrhage. The potential mechani...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.1044198/full |
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| author | Jingmei Ma Jingmei Ma Jingmei Ma Yawei Liu Yawei Liu Yawei Liu Yawei Liu Yawei Liu Zhirong Guo Zhirong Guo Run Sun Run Sun Run Sun Run Sun Xinrui Yang Xinrui Yang Weiran Zheng Weiran Zheng Yongdan Ma Yongdan Ma Yin Rong Yin Rong Yin Rong Yin Rong Hongmei Wang Hongmei Wang Hongmei Wang Hongmei Wang Huixia Yang Huixia Yang Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao |
| author_facet | Jingmei Ma Jingmei Ma Jingmei Ma Yawei Liu Yawei Liu Yawei Liu Yawei Liu Yawei Liu Zhirong Guo Zhirong Guo Run Sun Run Sun Run Sun Run Sun Xinrui Yang Xinrui Yang Weiran Zheng Weiran Zheng Yongdan Ma Yongdan Ma Yin Rong Yin Rong Yin Rong Yin Rong Hongmei Wang Hongmei Wang Hongmei Wang Hongmei Wang Huixia Yang Huixia Yang Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao |
| author_sort | Jingmei Ma |
| collection | DOAJ |
| description | Placenta accreta spectrum disorders (PAS) are severe pregnancy complications that occur when extravillous trophoblast cells (EVTs) invade beyond the uterine inner myometrium and are characterized by hypervascularity on prenatal ultrasound and catastrophic postpartum hemorrhage. The potential mechanisms remain incompletely understood. With single-cell RNA-sequencing analysis on the representative invasive parts and the normal part obtained from the same PAS placenta, we profiled the pathological landscape of invasive PAS placenta and deciphered an intensified differentiation pathway from progenitor cytotrophoblasts (CTBs) to EVTs via LAMB4+ and KRT6A+ CTBs. In the absence of the decidua, the invasive trophoblasts of various differentiation states interacted with ADIRF+ and DES+ maternal stromal cells. The PAS-associated hypervascularity might be due to the enhanced crosstalk of trophoblasts, stromal cells and vascular endothelial cells. Finally, we presented an immune microenvironmental landscape of invasive PAS. The pathogenesis of PAS could be further explored with current resources for future targeted translational studies. |
| first_indexed | 2024-04-12T08:37:12Z |
| format | Article |
| id | doaj.art-413597831c214ed38ce4559af084407b |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-12T08:37:12Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-413597831c214ed38ce4559af084407b2022-12-22T03:39:59ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-11-011010.3389/fcell.2022.10441981044198The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencingJingmei Ma0Jingmei Ma1Jingmei Ma2Yawei Liu3Yawei Liu4Yawei Liu5Yawei Liu6Yawei Liu7Zhirong Guo8Zhirong Guo9Run Sun10Run Sun11Run Sun12Run Sun13Xinrui Yang14Xinrui Yang15Weiran Zheng16Weiran Zheng17Yongdan Ma18Yongdan Ma19Yin Rong20Yin Rong21Yin Rong22Yin Rong23Hongmei Wang24Hongmei Wang25Hongmei Wang26Hongmei Wang27Huixia Yang28Huixia Yang29Zhenyu Xiao30Zhenyu Xiao31Zhenyu Xiao32Zhenyu Xiao33Zhenyu Xiao34State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDushu Lake Hospital Affiliated to Soochow University, Medical Center of Soochow University, Suzhou Dushu Lake Hospital, Suzhou, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University First Hospital, Beijing, ChinaBeijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInnovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaSchool of Life Science, Beijing Institute of Technology, Beijing, ChinaPlacenta accreta spectrum disorders (PAS) are severe pregnancy complications that occur when extravillous trophoblast cells (EVTs) invade beyond the uterine inner myometrium and are characterized by hypervascularity on prenatal ultrasound and catastrophic postpartum hemorrhage. The potential mechanisms remain incompletely understood. With single-cell RNA-sequencing analysis on the representative invasive parts and the normal part obtained from the same PAS placenta, we profiled the pathological landscape of invasive PAS placenta and deciphered an intensified differentiation pathway from progenitor cytotrophoblasts (CTBs) to EVTs via LAMB4+ and KRT6A+ CTBs. In the absence of the decidua, the invasive trophoblasts of various differentiation states interacted with ADIRF+ and DES+ maternal stromal cells. The PAS-associated hypervascularity might be due to the enhanced crosstalk of trophoblasts, stromal cells and vascular endothelial cells. Finally, we presented an immune microenvironmental landscape of invasive PAS. The pathogenesis of PAS could be further explored with current resources for future targeted translational studies.https://www.frontiersin.org/articles/10.3389/fcell.2022.1044198/fullplacentatrophoblastsingle-cell sequencingpathogenesisplacenta accreta spectrum |
| spellingShingle | Jingmei Ma Jingmei Ma Jingmei Ma Yawei Liu Yawei Liu Yawei Liu Yawei Liu Yawei Liu Zhirong Guo Zhirong Guo Run Sun Run Sun Run Sun Run Sun Xinrui Yang Xinrui Yang Weiran Zheng Weiran Zheng Yongdan Ma Yongdan Ma Yin Rong Yin Rong Yin Rong Yin Rong Hongmei Wang Hongmei Wang Hongmei Wang Hongmei Wang Huixia Yang Huixia Yang Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao Zhenyu Xiao The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing Frontiers in Cell and Developmental Biology placenta trophoblast single-cell sequencing pathogenesis placenta accreta spectrum |
| title | The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing |
| title_full | The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing |
| title_fullStr | The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing |
| title_full_unstemmed | The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing |
| title_short | The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing |
| title_sort | diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single cell rna sequencing |
| topic | placenta trophoblast single-cell sequencing pathogenesis placenta accreta spectrum |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.1044198/full |
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