Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose
Jing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Prov...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-04-01
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| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/silenced-snhg1-inhibited-epithelial-mesenchymal-transition-and-inflamm-peer-reviewed-fulltext-article-JIR |
| _version_ | 1818754014850842624 |
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| author | Yang J Yang K Meng X Liu P Fu Y Wang Y |
| author_facet | Yang J Yang K Meng X Liu P Fu Y Wang Y |
| author_sort | Yang J |
| collection | DOAJ |
| description | Jing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of ChinaCorrespondence: Xuxia Meng Email mengxx@qduhospital.cnPurpose: The lncRNA small nucleolar RNA host gene 1 (SNHG1) is a cerebral infarction-associated gene, its biological role and mechanism in diabetic retinopathy remain to be illuminated. The present study was designed to investigate the role of SNHG1 in high glucose induced human retinal pigment epithelial cells (ARPE-19).Methods: ARPE-19 cells were cultured and exposed to 60 mM high glucose for 48h, and 5.5mM glucose-exposed ARPE-19 cells were used as the control. The levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin, ZO-1, vimentin and α-SMA were measured, and the Cell inflammatory response was evaluated by detecting IL-6 and IL-1β levels. Then, cell migration, proliferation and apoptosis were detected. The expression of the lncRNA SNHG1 in ARPE-19 cells was detected by quantitative real-time PCR. SNHG1 was knocked down by small interfering RNA (siRNA) transfection. The effects of SNHG1 inhibition on inflammation, EMT, migration, proliferation and apoptosis were observed.Results: The results showed that the expression of SNHG1 was significantly increased in ARPE-19 cells exposed to high glucose. Silencing SNHG1 reduced the expression of vimentin, α-SMA, and the expression of inflammatory chemokines IL-6 and IL-1β, inhibited migration and proliferation, elevated the expression of E-cadherin and ZO-1, and promoted apoptosis in ARPE-19 cells.Conclusion: The lncRNA SNHG1 is involved in hyperglycemia-induced EMT and the inflammatory response of ARPE-19 cells and provides a new understanding of the pathogenesis of DR.Keywords: epithelial-mesenchymal transition, small nucleolar RNA host gene 1, retinal pigment epithelial, hyperglycemia |
| first_indexed | 2024-12-18T05:16:31Z |
| format | Article |
| id | doaj.art-4136b14ecef440428e1d2a25ccb455c1 |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-12-18T05:16:31Z |
| publishDate | 2021-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-4136b14ecef440428e1d2a25ccb455c12022-12-21T21:19:45ZengDove Medical PressJournal of Inflammation Research1178-70312021-04-01Volume 141563157364074Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High GlucoseYang JYang KMeng XLiu PFu YWang YJing Yang,1 Kun Yang,2 Xuxia Meng,1 Penghui Liu,1 Yudong Fu,1 Yibo Wang1 1Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of China; 2Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, 266500, Shandong Province, People’s Republic of ChinaCorrespondence: Xuxia Meng Email mengxx@qduhospital.cnPurpose: The lncRNA small nucleolar RNA host gene 1 (SNHG1) is a cerebral infarction-associated gene, its biological role and mechanism in diabetic retinopathy remain to be illuminated. The present study was designed to investigate the role of SNHG1 in high glucose induced human retinal pigment epithelial cells (ARPE-19).Methods: ARPE-19 cells were cultured and exposed to 60 mM high glucose for 48h, and 5.5mM glucose-exposed ARPE-19 cells were used as the control. The levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin, ZO-1, vimentin and α-SMA were measured, and the Cell inflammatory response was evaluated by detecting IL-6 and IL-1β levels. Then, cell migration, proliferation and apoptosis were detected. The expression of the lncRNA SNHG1 in ARPE-19 cells was detected by quantitative real-time PCR. SNHG1 was knocked down by small interfering RNA (siRNA) transfection. The effects of SNHG1 inhibition on inflammation, EMT, migration, proliferation and apoptosis were observed.Results: The results showed that the expression of SNHG1 was significantly increased in ARPE-19 cells exposed to high glucose. Silencing SNHG1 reduced the expression of vimentin, α-SMA, and the expression of inflammatory chemokines IL-6 and IL-1β, inhibited migration and proliferation, elevated the expression of E-cadherin and ZO-1, and promoted apoptosis in ARPE-19 cells.Conclusion: The lncRNA SNHG1 is involved in hyperglycemia-induced EMT and the inflammatory response of ARPE-19 cells and provides a new understanding of the pathogenesis of DR.Keywords: epithelial-mesenchymal transition, small nucleolar RNA host gene 1, retinal pigment epithelial, hyperglycemiahttps://www.dovepress.com/silenced-snhg1-inhibited-epithelial-mesenchymal-transition-and-inflamm-peer-reviewed-fulltext-article-JIRepithelial-mesenchymal transitionsmall nucleolar rna host gene 1retinal pigment epithelialhyperglycemia |
| spellingShingle | Yang J Yang K Meng X Liu P Fu Y Wang Y Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose Journal of Inflammation Research epithelial-mesenchymal transition small nucleolar rna host gene 1 retinal pigment epithelial hyperglycemia |
| title | Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose |
| title_full | Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose |
| title_fullStr | Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose |
| title_full_unstemmed | Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose |
| title_short | Silenced SNHG1 Inhibited Epithelial-Mesenchymal Transition and Inflammatory Response of ARPE-19 Cells Induced by High Glucose |
| title_sort | silenced snhg1 inhibited epithelial mesenchymal transition and inflammatory response of arpe 19 cells induced by high glucose |
| topic | epithelial-mesenchymal transition small nucleolar rna host gene 1 retinal pigment epithelial hyperglycemia |
| url | https://www.dovepress.com/silenced-snhg1-inhibited-epithelial-mesenchymal-transition-and-inflamm-peer-reviewed-fulltext-article-JIR |
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