Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway
AbstractTaccalonolide A has been reported to have anti-tumour efficiency. However, the underlying mechanism for taccalonolides A therapy of hepatocellular carcinoma (HCC) is still obscure. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Apoptosis was determined by flow cytometry....
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1773484 |
| _version_ | 1797788795072937984 |
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| author | Hui Tian Zhenkun He |
| author_facet | Hui Tian Zhenkun He |
| author_sort | Hui Tian |
| collection | DOAJ |
| description | AbstractTaccalonolide A has been reported to have anti-tumour efficiency. However, the underlying mechanism for taccalonolides A therapy of hepatocellular carcinoma (HCC) is still obscure. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Apoptosis was determined by flow cytometry. Protein expression of B cell lymphoma (Bcl-2), Bcl-2 associated X (Bax), sonic hedgehog (Shh), Smoothened (Smo) and Gli family zinc finger 1 (Gli1) was analyzed by western blot. The expression of Shh, Smo and Gli1 mRNA was determined using quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that taccalonolide A inhibited cell proliferation, induced apoptosis and cell cycle arrest at the G0/G1 phase, and improved the cytotoxicity of sorafenib in HCC cells. The expressions of Shh, Smo, Gli1 mRNA and protein were decreased after taccalonolide A treatment. More importantly, activation of the Shh pathway attenuated taccalonolide A-induced inhibition on cell viability and promotion on apoptosis and cell cycle arrest in HCC. Also, activation of the Shh pathway neutralized the effect of taccalonolide A on sorafenib cytotoxicity in HCC. We clarified that taccalonolide A suppressed cell viability facilitated apoptosis, and improved the cytotoxicity of sorafenib in HCC by inhibition of the activation of the Shh pathway, providing alternative treatments for HCC. |
| first_indexed | 2024-03-13T01:40:33Z |
| format | Article |
| id | doaj.art-4137575516e641d19ecaaa3aa937579d |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-13T01:40:33Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-4137575516e641d19ecaaa3aa937579d2023-07-03T14:04:56ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148193994710.1080/21691401.2020.1773484Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathwayHui Tian0Zhenkun He1Department of Infectious Disease, Huaihe Hospital of Henan University, Kaifeng, Henan, ChinaDepartment of Infectious Disease, Huaihe Hospital of Henan University, Kaifeng, Henan, ChinaAbstractTaccalonolide A has been reported to have anti-tumour efficiency. However, the underlying mechanism for taccalonolides A therapy of hepatocellular carcinoma (HCC) is still obscure. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Apoptosis was determined by flow cytometry. Protein expression of B cell lymphoma (Bcl-2), Bcl-2 associated X (Bax), sonic hedgehog (Shh), Smoothened (Smo) and Gli family zinc finger 1 (Gli1) was analyzed by western blot. The expression of Shh, Smo and Gli1 mRNA was determined using quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that taccalonolide A inhibited cell proliferation, induced apoptosis and cell cycle arrest at the G0/G1 phase, and improved the cytotoxicity of sorafenib in HCC cells. The expressions of Shh, Smo, Gli1 mRNA and protein were decreased after taccalonolide A treatment. More importantly, activation of the Shh pathway attenuated taccalonolide A-induced inhibition on cell viability and promotion on apoptosis and cell cycle arrest in HCC. Also, activation of the Shh pathway neutralized the effect of taccalonolide A on sorafenib cytotoxicity in HCC. We clarified that taccalonolide A suppressed cell viability facilitated apoptosis, and improved the cytotoxicity of sorafenib in HCC by inhibition of the activation of the Shh pathway, providing alternative treatments for HCC.https://www.tandfonline.com/doi/10.1080/21691401.2020.1773484Taccalonolide AsorafenibShh pathwayhepatocellular carcinoma |
| spellingShingle | Hui Tian Zhenkun He Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway Artificial Cells, Nanomedicine, and Biotechnology Taccalonolide A sorafenib Shh pathway hepatocellular carcinoma |
| title | Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway |
| title_full | Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway |
| title_fullStr | Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway |
| title_full_unstemmed | Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway |
| title_short | Anti-hepatoma effect of taccalonolide A through suppression of sonic hedgehog pathway |
| title_sort | anti hepatoma effect of taccalonolide a through suppression of sonic hedgehog pathway |
| topic | Taccalonolide A sorafenib Shh pathway hepatocellular carcinoma |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1773484 |
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