Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection
Systemic therapy with oxytetracycline is often used for treatment of clinical metritis although data about its penetration into the uterus and uterine secretion are lacking. Uterine secretions and milk from six cows with clinical metritis were collected for microbiological assay. The animals were tr...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/7/392 |
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| author | Rositsa Mileva Manol Karadaev Ivan Fasulkov Tsvetelina Petkova Nikolina Rusenova Nasko Vasilev Aneliya Milanova |
| author_facet | Rositsa Mileva Manol Karadaev Ivan Fasulkov Tsvetelina Petkova Nikolina Rusenova Nasko Vasilev Aneliya Milanova |
| author_sort | Rositsa Mileva |
| collection | DOAJ |
| description | Systemic therapy with oxytetracycline is often used for treatment of clinical metritis although data about its penetration into the uterus and uterine secretion are lacking. Uterine secretions and milk from six cows with clinical metritis were collected for microbiological assay. The animals were treated intramuscularly with long-acting oxytetracycline (20 mg/kg) and samples of plasma, milk and uterine secretions were collected for determination of the antibiotic concentrations by HPLC-PDA analysis. Pharmacokinetics of the antibiotic and in silico prediction of its penetration into the uterus were described. <i>Trueperella pyogenes</i> with MIC values of 16–64 µg mL<sup>−1</sup> was isolated (<i>n</i> of cows = 4) from uterine secretions. Oxytetracycline showed fast absorption and penetration in the uterine secretions and milk. No change of withdrawal time for milk was necessitated in cows with clinical metritis. Maximum levels in uterine secretions and predicted concentrations of oxytetracycline in the uterus were lower than MIC values. Systemic administration of long-acting oxytetracycline did not guarantee clinical cure and was not a suitable choice for treatment of clinical metritis associated with <i>Trueperella pyogenes</i>. The appropriate approach to antibiotic treatment of uterine infections of cows requires knowledge on penetration of the antibiotics at the site of infection and sensitivity of pathogens. |
| first_indexed | 2024-03-10T18:35:25Z |
| format | Article |
| id | doaj.art-41386dfd0ee04061bafbe99c437defd5 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T18:35:25Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-41386dfd0ee04061bafbe99c437defd52023-11-20T06:14:46ZengMDPI AGAntibiotics2079-63822020-07-019739210.3390/antibiotics9070392Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> InfectionRositsa Mileva0Manol Karadaev1Ivan Fasulkov2Tsvetelina Petkova3Nikolina Rusenova4Nasko Vasilev5Aneliya Milanova6Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaSystemic therapy with oxytetracycline is often used for treatment of clinical metritis although data about its penetration into the uterus and uterine secretion are lacking. Uterine secretions and milk from six cows with clinical metritis were collected for microbiological assay. The animals were treated intramuscularly with long-acting oxytetracycline (20 mg/kg) and samples of plasma, milk and uterine secretions were collected for determination of the antibiotic concentrations by HPLC-PDA analysis. Pharmacokinetics of the antibiotic and in silico prediction of its penetration into the uterus were described. <i>Trueperella pyogenes</i> with MIC values of 16–64 µg mL<sup>−1</sup> was isolated (<i>n</i> of cows = 4) from uterine secretions. Oxytetracycline showed fast absorption and penetration in the uterine secretions and milk. No change of withdrawal time for milk was necessitated in cows with clinical metritis. Maximum levels in uterine secretions and predicted concentrations of oxytetracycline in the uterus were lower than MIC values. Systemic administration of long-acting oxytetracycline did not guarantee clinical cure and was not a suitable choice for treatment of clinical metritis associated with <i>Trueperella pyogenes</i>. The appropriate approach to antibiotic treatment of uterine infections of cows requires knowledge on penetration of the antibiotics at the site of infection and sensitivity of pathogens.https://www.mdpi.com/2079-6382/9/7/392oxytetracyclinepharmacokineticscowsclinical metritis<i>Trueperella pyogenes</i> |
| spellingShingle | Rositsa Mileva Manol Karadaev Ivan Fasulkov Tsvetelina Petkova Nikolina Rusenova Nasko Vasilev Aneliya Milanova Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection Antibiotics oxytetracycline pharmacokinetics cows clinical metritis <i>Trueperella pyogenes</i> |
| title | Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection |
| title_full | Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection |
| title_fullStr | Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection |
| title_full_unstemmed | Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection |
| title_short | Oxytetracycline Pharmacokinetics After Intramuscular Administration in Cows with Clinical Metritis Associated with <i>Trueperella Pyogenes</i> Infection |
| title_sort | oxytetracycline pharmacokinetics after intramuscular administration in cows with clinical metritis associated with i trueperella pyogenes i infection |
| topic | oxytetracycline pharmacokinetics cows clinical metritis <i>Trueperella pyogenes</i> |
| url | https://www.mdpi.com/2079-6382/9/7/392 |
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