Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq
BackgroundSalt-sensitive hypertension (SSH) is a common type of essential hypertension in China. In recent years, although an increasing number of researches have focused on SSH, few studies have been researched on patients with SSH. The objective of this study was to explore the genes and pathways...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.963744/full |
| _version_ | 1798037767552237568 |
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| author | Xiaoying Chao Zhiyuan Jiang Guoqiang Zhong Rongjie Huang |
| author_facet | Xiaoying Chao Zhiyuan Jiang Guoqiang Zhong Rongjie Huang |
| author_sort | Xiaoying Chao |
| collection | DOAJ |
| description | BackgroundSalt-sensitive hypertension (SSH) is a common type of essential hypertension in China. In recent years, although an increasing number of researches have focused on SSH, few studies have been researched on patients with SSH. The objective of this study was to explore the genes and pathways linked with SSH using RNA-sequencing (RNA-seq).Materials and methodsWe used RNA-seq to analyze the transcriptome of peripheral blood mononuclear cells (PBMCs) of five SSH patients and five SRH patients. Next, we analyzed the differentially expressed genes (DEGs) using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Gene Set Enrichment (GSEA) enrichment analysis. Then, Cytoscape was used to construct the protein-protein interaction (PPI) network and the hub genes. Finally, CMAP analysis found that several small molecular compounds could reverse the altered DEGs.ResultsA total of 431 DEGs were found in the PBMC samples, including 294 up-regulated and 137 down-regulated genes. Functional enrichment analysis found significant enrichment in immune-related associations such as inflammation, chemokine, and cytokine-cytokine receptor interaction. The hub genes of the two modules were IL-6, IL-1A, CCL2, CCL3L3, and BUB1. In addition, we identified two small molecular compounds (iopromide and iloprost) that potentially interacted with DEGs.ConclusionThis study suggests some potential biomarkers for the diagnosis of SSH. It provides new insights into SSH diagnosis and possible future clinical treatment. |
| first_indexed | 2024-04-11T21:31:03Z |
| format | Article |
| id | doaj.art-41398db176524dcfb9a33a36e8f7649b |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-11T21:31:03Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-41398db176524dcfb9a33a36e8f7649b2022-12-22T04:01:57ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-08-01910.3389/fcvm.2022.963744963744Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seqXiaoying Chao0Zhiyuan Jiang1Guoqiang Zhong2Rongjie Huang3Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDivision of Hypertension, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDivision of Hypertension, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaBackgroundSalt-sensitive hypertension (SSH) is a common type of essential hypertension in China. In recent years, although an increasing number of researches have focused on SSH, few studies have been researched on patients with SSH. The objective of this study was to explore the genes and pathways linked with SSH using RNA-sequencing (RNA-seq).Materials and methodsWe used RNA-seq to analyze the transcriptome of peripheral blood mononuclear cells (PBMCs) of five SSH patients and five SRH patients. Next, we analyzed the differentially expressed genes (DEGs) using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Gene Set Enrichment (GSEA) enrichment analysis. Then, Cytoscape was used to construct the protein-protein interaction (PPI) network and the hub genes. Finally, CMAP analysis found that several small molecular compounds could reverse the altered DEGs.ResultsA total of 431 DEGs were found in the PBMC samples, including 294 up-regulated and 137 down-regulated genes. Functional enrichment analysis found significant enrichment in immune-related associations such as inflammation, chemokine, and cytokine-cytokine receptor interaction. The hub genes of the two modules were IL-6, IL-1A, CCL2, CCL3L3, and BUB1. In addition, we identified two small molecular compounds (iopromide and iloprost) that potentially interacted with DEGs.ConclusionThis study suggests some potential biomarkers for the diagnosis of SSH. It provides new insights into SSH diagnosis and possible future clinical treatment.https://www.frontiersin.org/articles/10.3389/fcvm.2022.963744/fullRNA-sequencingdifferentially expressed genesbiomarkersalt-sensitive hypertensionsalt-resistant hypertension |
| spellingShingle | Xiaoying Chao Zhiyuan Jiang Guoqiang Zhong Rongjie Huang Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq Frontiers in Cardiovascular Medicine RNA-sequencing differentially expressed genes biomarker salt-sensitive hypertension salt-resistant hypertension |
| title | Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq |
| title_full | Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq |
| title_fullStr | Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq |
| title_full_unstemmed | Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq |
| title_short | Identification of biomarkers, pathways and potential therapeutic agents for salt-sensitive hypertension using RNA-seq |
| title_sort | identification of biomarkers pathways and potential therapeutic agents for salt sensitive hypertension using rna seq |
| topic | RNA-sequencing differentially expressed genes biomarker salt-sensitive hypertension salt-resistant hypertension |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.963744/full |
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