Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction
In order to solve the problems of the detection of various foodborne stimulants in different animal-derived foods such as significant differences in matrix effects and many types of interference, a method was established for the determination of 10 protein anabolic agents, 8 glucocorticoids, and 12...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2024-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-033.pdf |
| _version_ | 1797256617099526144 |
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| author | LIU Chuan, LI Jiajia, WU Xueying, CHEN Yanqiu, SHI Peiyu, SONG Juan, DAI Qin |
| author_facet | LIU Chuan, LI Jiajia, WU Xueying, CHEN Yanqiu, SHI Peiyu, SONG Juan, DAI Qin |
| author_sort | LIU Chuan, LI Jiajia, WU Xueying, CHEN Yanqiu, SHI Peiyu, SONG Juan, DAI Qin |
| collection | DOAJ |
| description | In order to solve the problems of the detection of various foodborne stimulants in different animal-derived foods such as significant differences in matrix effects and many types of interference, a method was established for the determination of 10 protein anabolic agents, 8 glucocorticoids, and 12 diuretics in animal-derived foods by pass-through solid phase extraction (SPE) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was extracted with 5% formic acid in acetonitrile, and the extract was purified by pass-through solid phase extraction before being analyzed by UPLC-MS/MS. The internal standard method was used for quantification. The results showed that good linearity was observed for all analytes within their respective concentration ranges. The average recoveries ranged from 67.5% to 114.3% with relative standard deviations (RSDs) between 0.7% and 16.1% (n = 6). This method is characterized by high sensitivity, simple operation, high throughput and high detection efficiency, which improves the measurement accuracy of different complex matrices and can meet the need for the detection of the 30 foodborne stimulants in animal-derived foods used in sports events. |
| first_indexed | 2024-04-24T22:24:35Z |
| format | Article |
| id | doaj.art-413a977e6d1441b6b93ac18a8ff38c4a |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-24T22:24:35Z |
| publishDate | 2024-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-413a977e6d1441b6b93ac18a8ff38c4a2024-03-20T02:27:16ZengChina Food Publishing CompanyShipin Kexue1002-66302024-02-0145428929910.7506/spkx1002-6630-20230427-268Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase ExtractionLIU Chuan, LI Jiajia, WU Xueying, CHEN Yanqiu, SHI Peiyu, SONG Juan, DAI Qin0(1. Chengdu Institute of Food Inspection, Chengdu 611130, China; 2. Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Beijing 100029, China)In order to solve the problems of the detection of various foodborne stimulants in different animal-derived foods such as significant differences in matrix effects and many types of interference, a method was established for the determination of 10 protein anabolic agents, 8 glucocorticoids, and 12 diuretics in animal-derived foods by pass-through solid phase extraction (SPE) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample was extracted with 5% formic acid in acetonitrile, and the extract was purified by pass-through solid phase extraction before being analyzed by UPLC-MS/MS. The internal standard method was used for quantification. The results showed that good linearity was observed for all analytes within their respective concentration ranges. The average recoveries ranged from 67.5% to 114.3% with relative standard deviations (RSDs) between 0.7% and 16.1% (n = 6). This method is characterized by high sensitivity, simple operation, high throughput and high detection efficiency, which improves the measurement accuracy of different complex matrices and can meet the need for the detection of the 30 foodborne stimulants in animal-derived foods used in sports events.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-033.pdfultra-high performance liquid chromatography-tandem mass spectrometry; animal-derived foods; protein anabolic agents; glucocorticoids; diuretics |
| spellingShingle | LIU Chuan, LI Jiajia, WU Xueying, CHEN Yanqiu, SHI Peiyu, SONG Juan, DAI Qin Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction Shipin Kexue ultra-high performance liquid chromatography-tandem mass spectrometry; animal-derived foods; protein anabolic agents; glucocorticoids; diuretics |
| title | Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction |
| title_full | Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction |
| title_fullStr | Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction |
| title_full_unstemmed | Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction |
| title_short | Simultaneous Determination of 30 Foodborne Stimulant Drug Residues in Animal-Derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-Through Solid Phase Extraction |
| title_sort | simultaneous determination of 30 foodborne stimulant drug residues in animal derived foods by ultra high performance liquid chromatography tandem mass spectrometry with pass through solid phase extraction |
| topic | ultra-high performance liquid chromatography-tandem mass spectrometry; animal-derived foods; protein anabolic agents; glucocorticoids; diuretics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-033.pdf |
| work_keys_str_mv | AT liuchuanlijiajiawuxueyingchenyanqiushipeiyusongjuandaiqin simultaneousdeterminationof30foodbornestimulantdrugresiduesinanimalderivedfoodsbyultrahighperformanceliquidchromatographytandemmassspectrometrywithpassthroughsolidphaseextraction |