Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose
A novel ratiometric fluorescent probe was constructed for sensitive assay of hydrogen peroxide (H2O2) and glucose, which utilized the synergistically enhanced effects of Ce3+ and Fe2+ on copper nanoclusters (CuNCs) and coumarin. In the CuNCs-Ce3+/Fe2+-coumarin system, Ce3+ triggered the aggregation-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322009575 |
| _version_ | 1818032297196126208 |
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| author | He Mei Jianping Wang Xiaolei Zhu Juan Sun Wei Shi Huili Wang Shugen Qu Xuedong Wang |
| author_facet | He Mei Jianping Wang Xiaolei Zhu Juan Sun Wei Shi Huili Wang Shugen Qu Xuedong Wang |
| author_sort | He Mei |
| collection | DOAJ |
| description | A novel ratiometric fluorescent probe was constructed for sensitive assay of hydrogen peroxide (H2O2) and glucose, which utilized the synergistically enhanced effects of Ce3+ and Fe2+ on copper nanoclusters (CuNCs) and coumarin. In the CuNCs-Ce3+/Fe2+-coumarin system, Ce3+ triggered the aggregation-induced emission phenomenon of CuNCs, and Fe2+ catalyzed the Fenton reaction to efficiently yield hydroxyl radical (•OH). In the presence of H2O2, the 625-nm red fluorescence of CuNCs was sharply quenched owing to the oxidation of CuNCs to Cu(II) by •OH, but the 460-nm blue fluorescence of 7-hydroxycoumarin from the oxidation of coumarin by •OH dramatically increased. Based on the reversible changes in two fluorescence signals, a satisfactorily ratiometric probe was constructed for H2O2 assay with a detection limit (LOD) of 0.6 μM accompanied by a visual color variation from red to blue. For glucose assay, this ratiometric probe gave a linear range of 3.2–160 μM and LOD of 0.96 μM owing to the oxidization of glucose to yield H2O2 in the presence of glucose oxidase and O2. Overall, the newly developed ratiometric probe shows a great prospect in real applications for visual assay of H2O2 and glucose by our naked eyes. |
| first_indexed | 2024-12-10T06:05:08Z |
| format | Article |
| id | doaj.art-413b52e61a2c41dcb6a6250758c8964a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-10T06:05:08Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-413b52e61a2c41dcb6a6250758c8964a2022-12-22T01:59:43ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01245114117Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucoseHe Mei0Jianping Wang1Xiaolei Zhu2Juan Sun3Wei Shi4Huili Wang5Shugen Qu6Xuedong Wang7Center for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China; South Zhejiang Institute of Radiation Medicine and Nuclear Technology, Wenzhou 325809, ChinaCenter for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, ChinaCenter for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, ChinaCenter for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, ChinaCenter for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, ChinaSchool of Environmental Science and Technology, Suzhou University of Science and Technology, Suzhou 215009, ChinaCenter for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China; South Zhejiang Institute of Radiation Medicine and Nuclear Technology, Wenzhou 325809, China; Corresponding author at: Center for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China.Center for Health Assessment, Zhejiang Provincial Key Laboratory of Watershed Science and Health, College of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, China; Corresponding author.A novel ratiometric fluorescent probe was constructed for sensitive assay of hydrogen peroxide (H2O2) and glucose, which utilized the synergistically enhanced effects of Ce3+ and Fe2+ on copper nanoclusters (CuNCs) and coumarin. In the CuNCs-Ce3+/Fe2+-coumarin system, Ce3+ triggered the aggregation-induced emission phenomenon of CuNCs, and Fe2+ catalyzed the Fenton reaction to efficiently yield hydroxyl radical (•OH). In the presence of H2O2, the 625-nm red fluorescence of CuNCs was sharply quenched owing to the oxidation of CuNCs to Cu(II) by •OH, but the 460-nm blue fluorescence of 7-hydroxycoumarin from the oxidation of coumarin by •OH dramatically increased. Based on the reversible changes in two fluorescence signals, a satisfactorily ratiometric probe was constructed for H2O2 assay with a detection limit (LOD) of 0.6 μM accompanied by a visual color variation from red to blue. For glucose assay, this ratiometric probe gave a linear range of 3.2–160 μM and LOD of 0.96 μM owing to the oxidization of glucose to yield H2O2 in the presence of glucose oxidase and O2. Overall, the newly developed ratiometric probe shows a great prospect in real applications for visual assay of H2O2 and glucose by our naked eyes.http://www.sciencedirect.com/science/article/pii/S0147651322009575Ratiometric fluorescent probeCuNCs-coumarinCe3+Fe2+H2O2Glucose |
| spellingShingle | He Mei Jianping Wang Xiaolei Zhu Juan Sun Wei Shi Huili Wang Shugen Qu Xuedong Wang Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose Ecotoxicology and Environmental Safety Ratiometric fluorescent probe CuNCs-coumarin Ce3+ Fe2+ H2O2 Glucose |
| title | Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| title_full | Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| title_fullStr | Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| title_full_unstemmed | Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| title_short | Ce3+ and Fe2+ co-enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| title_sort | ce3 and fe2 co enhanced ratiometric fluorescence probe utilizing copper nanoclusters and coumarin for sensitive assay of hydrogen peroxide and glucose |
| topic | Ratiometric fluorescent probe CuNCs-coumarin Ce3+ Fe2+ H2O2 Glucose |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322009575 |
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