Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma
The current study provides an in-depth analysis of the biological properties of a Cu(II) complex (C<sub>22</sub>H<sub>24</sub>Cu<sub>2</sub>N<sub>6</sub>O<sub>10</sub>) obtained from an aryl-semicarbazone ligand derived (L) from the condens...
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MDPI AG
2024-01-01
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| Series: | Inorganics |
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| Online Access: | https://www.mdpi.com/2304-6740/12/1/19 |
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| author | Ribhu Maity Biplab Manna Swapan Maity Kalyanmoy Jana Tithi Maity Mohd Afzal Nayim Sepay Bidhan Chandra Samanta |
| author_facet | Ribhu Maity Biplab Manna Swapan Maity Kalyanmoy Jana Tithi Maity Mohd Afzal Nayim Sepay Bidhan Chandra Samanta |
| author_sort | Ribhu Maity |
| collection | DOAJ |
| description | The current study provides an in-depth analysis of the biological properties of a Cu(II) complex (C<sub>22</sub>H<sub>24</sub>Cu<sub>2</sub>N<sub>6</sub>O<sub>10</sub>) obtained from an aryl-semicarbazone ligand derived (L) from the condensation of 2,4-dihydroxy acetophenone and semicarbazide. The binding behavior of this complex with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) protein was explored using a combination of experimental and theoretical approaches. The results suggest that the complex binds with CT-DNA via a partial intercalation, and hydrophobic interaction. However, the complex binds to BSA protein predominantly through hydrogen bonding or van der Waals interactions rather than hydrophobic interactions. The molecular docking methodology was carried out to substantiate the experimental finding. Furthermore, the in vitro cytotoxicity study was conducted on human cervix uteri carcinoma (SiHa cancerous cell) lines upon exposure to the complex, and the findings reveal a considerable decrease in cell viability, when compared to the control. Overall, this study provides a comprehensive understanding of the biological potential of the Cu(II) complex and its potential as an anti-cancer agent. |
| first_indexed | 2024-03-08T10:47:04Z |
| format | Article |
| id | doaj.art-413d9ecb6a0343f1a3765ebd44ca542d |
| institution | Directory Open Access Journal |
| issn | 2304-6740 |
| language | English |
| last_indexed | 2024-03-08T10:47:04Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Inorganics |
| spelling | doaj.art-413d9ecb6a0343f1a3765ebd44ca542d2024-01-26T17:04:01ZengMDPI AGInorganics2304-67402024-01-011211910.3390/inorganics12010019Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri CarcinomaRibhu Maity0Biplab Manna1Swapan Maity2Kalyanmoy Jana3Tithi Maity4Mohd Afzal5Nayim Sepay6Bidhan Chandra Samanta7Department of Chemistry, Mugberia Gangadhar Mahavidyalaya, Bhupatinagar, Purba Medinipur 721425, West Bengal, IndiaDepartment of Chemistry, Mugberia Gangadhar Mahavidyalaya, Bhupatinagar, Purba Medinipur 721425, West Bengal, IndiaSchool of Materials Science and Technology (SMST), Indian Institute of Technology (IIT), Banaras Hindu University, Varanasi 221005, Uttar Pradesh, IndiaDepartment of Chemistry, Mugberia Gangadhar Mahavidyalaya, Bhupatinagar, Purba Medinipur 721425, West Bengal, IndiaDepartment of Chemistry, Prabhat Kumar College, Contai 721401, West Bengal, IndiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, Lady Brabourne College, Kolkata 700017, West Bengal, IndiaDepartment of Chemistry, Mugberia Gangadhar Mahavidyalaya, Bhupatinagar, Purba Medinipur 721425, West Bengal, IndiaThe current study provides an in-depth analysis of the biological properties of a Cu(II) complex (C<sub>22</sub>H<sub>24</sub>Cu<sub>2</sub>N<sub>6</sub>O<sub>10</sub>) obtained from an aryl-semicarbazone ligand derived (L) from the condensation of 2,4-dihydroxy acetophenone and semicarbazide. The binding behavior of this complex with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) protein was explored using a combination of experimental and theoretical approaches. The results suggest that the complex binds with CT-DNA via a partial intercalation, and hydrophobic interaction. However, the complex binds to BSA protein predominantly through hydrogen bonding or van der Waals interactions rather than hydrophobic interactions. The molecular docking methodology was carried out to substantiate the experimental finding. Furthermore, the in vitro cytotoxicity study was conducted on human cervix uteri carcinoma (SiHa cancerous cell) lines upon exposure to the complex, and the findings reveal a considerable decrease in cell viability, when compared to the control. Overall, this study provides a comprehensive understanding of the biological potential of the Cu(II) complex and its potential as an anti-cancer agent.https://www.mdpi.com/2304-6740/12/1/19Cu(II) complexaryl-semicarbazone ligandDNA/BSA interactioncytotoxicitySiHa cancer cell |
| spellingShingle | Ribhu Maity Biplab Manna Swapan Maity Kalyanmoy Jana Tithi Maity Mohd Afzal Nayim Sepay Bidhan Chandra Samanta Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma Inorganics Cu(II) complex aryl-semicarbazone ligand DNA/BSA interaction cytotoxicity SiHa cancer cell |
| title | Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma |
| title_full | Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma |
| title_fullStr | Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma |
| title_full_unstemmed | Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma |
| title_short | Synthesis of an Aryl-Semicarbazone-Based Cu(II) Complex for DNA and BSA Interaction and Anti-Cancer Activity against Human Cervix Uteri Carcinoma |
| title_sort | synthesis of an aryl semicarbazone based cu ii complex for dna and bsa interaction and anti cancer activity against human cervix uteri carcinoma |
| topic | Cu(II) complex aryl-semicarbazone ligand DNA/BSA interaction cytotoxicity SiHa cancer cell |
| url | https://www.mdpi.com/2304-6740/12/1/19 |
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