The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender
The aims of this experiment was to obtain the effects of different time of glycerolisation on the quality of frozen ram semen diluted in Tris Egg Yolk (TEY). Semen from seven sexually mature local rams were collected using artificial vagina weekly, evaluated macroscopically and microscopically. ...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science
2006-09-01
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| Series: | Animal Production: Indonesian Journal of Animal Production |
| Online Access: | http://animalproduction.net/index.php/JAP/article/view/135 |
| _version_ | 1827298621989584896 |
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| author | T Laswardi Yusuf RI Arifiantini Y Mulyadi |
| author_facet | T Laswardi Yusuf RI Arifiantini Y Mulyadi |
| author_sort | T Laswardi Yusuf |
| collection | DOAJ |
| description | The aims of this experiment was to obtain the effects of different time of glycerolisation on the quality of frozen ram semen diluted in Tris Egg Yolk (TEY). Semen from seven sexually mature local rams were collected using artificial vagina weekly, evaluated macroscopically and microscopically. Good quality semen then diluted with TEY and 6% glycerol added in three different periods. The first step was diluted in room temperature ( treatment 1), the second glycerolisation was at the end of equilibration (treatment 2) and the third period was several steps of glycerolisation during equilibration (treatment 3). Diluted semen was packed in straws (Minitub, Germany), equilibrated at 5oC for three hours, freezed in liquid nitrogen vapor for 10 minutes and then stored in a container of -196 oC liquid nitrogen. After 24 hours, the semen was thawed at 37oC for 30 second. The results of showed that there was no significant differences among treatments (P>0.05). The percentage of post thawing motility was 42.50% (treatment 1), compared to 38.75% (treatment 2), and 30.00% (treatment 3). In conclusion, the glycerolization on frozen ram semen can be done any time of the equilibration without influencing the sperm motility. (Animal Production 8(3): 168-173 (2006)
Key Words: Glycerolisation, equilibration, ram semen |
| first_indexed | 2024-04-24T15:19:05Z |
| format | Article |
| id | doaj.art-413f9a1a4ee5430a808cb021a77d8166 |
| institution | Directory Open Access Journal |
| issn | 2541-5875 2541-5875 |
| language | English |
| last_indexed | 2024-04-24T15:19:05Z |
| publishDate | 2006-09-01 |
| publisher | Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science |
| record_format | Article |
| series | Animal Production: Indonesian Journal of Animal Production |
| spelling | doaj.art-413f9a1a4ee5430a808cb021a77d81662024-04-02T08:33:10ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production2541-58752541-58752006-09-0183123The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk ExtenderT Laswardi YusufRI ArifiantiniY MulyadiThe aims of this experiment was to obtain the effects of different time of glycerolisation on the quality of frozen ram semen diluted in Tris Egg Yolk (TEY). Semen from seven sexually mature local rams were collected using artificial vagina weekly, evaluated macroscopically and microscopically. Good quality semen then diluted with TEY and 6% glycerol added in three different periods. The first step was diluted in room temperature ( treatment 1), the second glycerolisation was at the end of equilibration (treatment 2) and the third period was several steps of glycerolisation during equilibration (treatment 3). Diluted semen was packed in straws (Minitub, Germany), equilibrated at 5oC for three hours, freezed in liquid nitrogen vapor for 10 minutes and then stored in a container of -196 oC liquid nitrogen. After 24 hours, the semen was thawed at 37oC for 30 second. The results of showed that there was no significant differences among treatments (P>0.05). The percentage of post thawing motility was 42.50% (treatment 1), compared to 38.75% (treatment 2), and 30.00% (treatment 3). In conclusion, the glycerolization on frozen ram semen can be done any time of the equilibration without influencing the sperm motility. (Animal Production 8(3): 168-173 (2006) Key Words: Glycerolisation, equilibration, ram semenhttp://animalproduction.net/index.php/JAP/article/view/135 |
| spellingShingle | T Laswardi Yusuf RI Arifiantini Y Mulyadi The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender Animal Production: Indonesian Journal of Animal Production |
| title | The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender |
| title_full | The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender |
| title_fullStr | The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender |
| title_full_unstemmed | The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender |
| title_short | The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender |
| title_sort | effect of glycerolisation time on the sperm motility of local ram frozen semen diluted in tris egg yolk extender |
| url | http://animalproduction.net/index.php/JAP/article/view/135 |
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