Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells
The potential of standard methods of radiation therapy is limited by the dose that can be safely delivered to the tumor, which could be too low for radical treatment. The dose efficiency can be increased by using radiosensitizers. In this study, we evaluated the sensitizing potential of biocompatibl...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Nhan Hau Tran Vyacheslav Ryzhov Andrey Volnitskiy Dmitry Amerkanov Fedor Pack Aleksander M. Golubev Alexandr Arutyunyan Anastasiia Spitsyna Vladimir Burdakov Dmitry Lebedev Andrey L. Konevega Tatiana Shtam Yaroslav Marchenko |
| author_facet | Nhan Hau Tran Vyacheslav Ryzhov Andrey Volnitskiy Dmitry Amerkanov Fedor Pack Aleksander M. Golubev Alexandr Arutyunyan Anastasiia Spitsyna Vladimir Burdakov Dmitry Lebedev Andrey L. Konevega Tatiana Shtam Yaroslav Marchenko |
| author_sort | Nhan Hau Tran |
| collection | DOAJ |
| description | The potential of standard methods of radiation therapy is limited by the dose that can be safely delivered to the tumor, which could be too low for radical treatment. The dose efficiency can be increased by using radiosensitizers. In this study, we evaluated the sensitizing potential of biocompatible iron oxide nanoparticles coated with a dextran shell in A172 and Gl-Tr glioblastoma cells in vitro. The cells preincubated with nanoparticles for 24 h were exposed to ionizing radiation (X-ray, gamma, or proton) at doses of 0.5–6 Gy, and their viability was assessed by the Resazurin assay and by staining of the surviving cells with crystal violet. A statistically significant effect of radiosensitization by nanoparticles was observed in both cell lines when cells were exposed to 35 keV X-rays. A weak radiosensitizing effect was found only in the Gl-Tr line for the 1.2 MeV gamma irradiation and there was no radiosensitizing effect in both lines for the 200 MeV proton irradiation at the Bragg peak. A slight (ca. 10%) increase in the formation of additional reactive oxygen species after X-ray irradiation was found when nanoparticles were present. These results suggest that the nanoparticles absorbed by glioma cells can produce a significant radiosensitizing effect, probably due to the action of secondary electrons generated by the magnetite core, whereas the dextran shell of the nanoparticles used in these experiments appears to be rather stable under radiation exposure. |
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| language | English |
| last_indexed | 2024-03-10T21:12:36Z |
| publishDate | 2023-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-414018990d9148478dda6d6d50f0f58a2023-11-19T16:42:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201515010.3390/ijms242015150Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma CellsNhan Hau Tran0Vyacheslav Ryzhov1Andrey Volnitskiy2Dmitry Amerkanov3Fedor Pack4Aleksander M. Golubev5Alexandr Arutyunyan6Anastasiia Spitsyna7Vladimir Burdakov8Dmitry Lebedev9Andrey L. Konevega10Tatiana Shtam11Yaroslav Marchenko12Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Orlova roscha 1, Gatchina 188300, RussiaThe potential of standard methods of radiation therapy is limited by the dose that can be safely delivered to the tumor, which could be too low for radical treatment. The dose efficiency can be increased by using radiosensitizers. In this study, we evaluated the sensitizing potential of biocompatible iron oxide nanoparticles coated with a dextran shell in A172 and Gl-Tr glioblastoma cells in vitro. The cells preincubated with nanoparticles for 24 h were exposed to ionizing radiation (X-ray, gamma, or proton) at doses of 0.5–6 Gy, and their viability was assessed by the Resazurin assay and by staining of the surviving cells with crystal violet. A statistically significant effect of radiosensitization by nanoparticles was observed in both cell lines when cells were exposed to 35 keV X-rays. A weak radiosensitizing effect was found only in the Gl-Tr line for the 1.2 MeV gamma irradiation and there was no radiosensitizing effect in both lines for the 200 MeV proton irradiation at the Bragg peak. A slight (ca. 10%) increase in the formation of additional reactive oxygen species after X-ray irradiation was found when nanoparticles were present. These results suggest that the nanoparticles absorbed by glioma cells can produce a significant radiosensitizing effect, probably due to the action of secondary electrons generated by the magnetite core, whereas the dextran shell of the nanoparticles used in these experiments appears to be rather stable under radiation exposure.https://www.mdpi.com/1422-0067/24/20/15150radiosensitizationsuperparamagnetic iron oxide nanoparticles (SPIONs)glioma cellsradiotherapy |
| spellingShingle | Nhan Hau Tran Vyacheslav Ryzhov Andrey Volnitskiy Dmitry Amerkanov Fedor Pack Aleksander M. Golubev Alexandr Arutyunyan Anastasiia Spitsyna Vladimir Burdakov Dmitry Lebedev Andrey L. Konevega Tatiana Shtam Yaroslav Marchenko Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells International Journal of Molecular Sciences radiosensitization superparamagnetic iron oxide nanoparticles (SPIONs) glioma cells radiotherapy |
| title | Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells |
| title_full | Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells |
| title_fullStr | Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells |
| title_full_unstemmed | Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells |
| title_short | Radiosensitizing Effect of Dextran-Coated Iron Oxide Nanoparticles on Malignant Glioma Cells |
| title_sort | radiosensitizing effect of dextran coated iron oxide nanoparticles on malignant glioma cells |
| topic | radiosensitization superparamagnetic iron oxide nanoparticles (SPIONs) glioma cells radiotherapy |
| url | https://www.mdpi.com/1422-0067/24/20/15150 |
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