Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity
Lignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of lignin peroxidase for environmental prevention and governance was promoted by exploring the effects of small molecule substances on lignin peroxidase activities. In this study, the lignin peroxi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/30/e3sconf_eppct2023_02036.pdf |
| _version_ | 1797807707608055808 |
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| author | Li Shouzhi Chen Yanzhen Liang Xiaoyu Ni Zhong Chen Huayou |
| author_facet | Li Shouzhi Chen Yanzhen Liang Xiaoyu Ni Zhong Chen Huayou |
| author_sort | Li Shouzhi |
| collection | DOAJ |
| description | Lignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of lignin peroxidase for environmental prevention and governance was promoted by exploring the effects of small molecule substances on lignin peroxidase activities. In this study, the lignin peroxidase (LiP) gene from Irpex Lacteus was heterologous expressed in Escherichia coli. The activity of the recombinant LiP was 11.2 U/mg, the optimum temperature was 37℃, and the optimum pH was 3.5. In the range of 20-45℃ and pH 2.0-4.0, the relative enzyme activity remained above 50%, showing good acid resistance, Zn2+, malonic acid, glycerol and other substances can promote the activity of LiP enzyme, among which Zn2+ can increase the activity of LiP enzyme to 256%, and when the glycerol supplemental level is 3%, the activity of LiP enzyme can increase to 186%. This study for the first time verified the promoting effect of glycerol on LiP enzyme activity, serving as a reference for LiP in studies on lignin breakdown and paper wastewater treatment. |
| first_indexed | 2024-03-13T06:26:39Z |
| format | Article |
| id | doaj.art-4144543712ca47fb8e4f0f6dcacdb054 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-13T06:26:39Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-4144543712ca47fb8e4f0f6dcacdb0542023-06-09T09:12:44ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013930203610.1051/e3sconf/202339302036e3sconf_eppct2023_02036Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activityLi Shouzhi0Chen Yanzhen1Liang Xiaoyu2Ni Zhong3Chen Huayou4School of Life Sciences, Jiangsu UniversitySchool of Life Sciences, Jiangsu UniversitySchool of Life Sciences, Jiangsu UniversitySchool of Life Sciences, Jiangsu UniversitySchool of Life Sciences, Jiangsu UniversityLignin peroxidase can degrade lignin in a relatively high efficiency, the highly efficient application of lignin peroxidase for environmental prevention and governance was promoted by exploring the effects of small molecule substances on lignin peroxidase activities. In this study, the lignin peroxidase (LiP) gene from Irpex Lacteus was heterologous expressed in Escherichia coli. The activity of the recombinant LiP was 11.2 U/mg, the optimum temperature was 37℃, and the optimum pH was 3.5. In the range of 20-45℃ and pH 2.0-4.0, the relative enzyme activity remained above 50%, showing good acid resistance, Zn2+, malonic acid, glycerol and other substances can promote the activity of LiP enzyme, among which Zn2+ can increase the activity of LiP enzyme to 256%, and when the glycerol supplemental level is 3%, the activity of LiP enzyme can increase to 186%. This study for the first time verified the promoting effect of glycerol on LiP enzyme activity, serving as a reference for LiP in studies on lignin breakdown and paper wastewater treatment.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/30/e3sconf_eppct2023_02036.pdf |
| spellingShingle | Li Shouzhi Chen Yanzhen Liang Xiaoyu Ni Zhong Chen Huayou Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity E3S Web of Conferences |
| title | Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity |
| title_full | Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity |
| title_fullStr | Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity |
| title_full_unstemmed | Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity |
| title_short | Expanding the application of lignin peroxidases from Irpex Lacteus in Lignin degradation by exploring the effects of small molecules on enzyme activity |
| title_sort | expanding the application of lignin peroxidases from irpex lacteus in lignin degradation by exploring the effects of small molecules on enzyme activity |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/30/e3sconf_eppct2023_02036.pdf |
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