Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation
Donor lymphocyte infusion (DLI) after allogeneic stem cell transplantation (alloSCT) is an established method to enhance the Graft-versus-Leukemia (GvL) effect. However, alterations of cellular subsets in the peripheral blood of DLI recipients have not been studied. We investigated the changes in ly...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Hemato |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-6357/2/4/46 |
| _version_ | 1797504132772265984 |
|---|---|
| author | Ann-Kristin Schmaelter Johanna Waidhauser Dina Kaiser Tatjana Lenskaja Stefanie Gruetzner Rainer Claus Martin Trepel Christoph Schmid Andreas Rank |
| author_facet | Ann-Kristin Schmaelter Johanna Waidhauser Dina Kaiser Tatjana Lenskaja Stefanie Gruetzner Rainer Claus Martin Trepel Christoph Schmid Andreas Rank |
| author_sort | Ann-Kristin Schmaelter |
| collection | DOAJ |
| description | Donor lymphocyte infusion (DLI) after allogeneic stem cell transplantation (alloSCT) is an established method to enhance the Graft-versus-Leukemia (GvL) effect. However, alterations of cellular subsets in the peripheral blood of DLI recipients have not been studied. We investigated the changes in lymphocyte subpopulations in 16 patients receiving DLI after successful alloSCT. Up to three DLIs were applied in escalating doses, prophylactically for relapse prevention in high-risk disease (<i>n</i> = 5), preemptively for mixed chimerism and/or a molecular relapse/persistence (<i>n</i> = 8), or as part of treatment for hematological relapse (<i>n</i> = 3). We used immunophenotyping to measure the absolute numbers of CD4+, CD8+, NK, and CD56+ T cells and their respective subsets in patients’ peripheral blood one day before DLI (d-1) and compared the results at day + 1 and + 7 post DLI to the values before DLI. After the administration of 1 × 10<sup>6</sup> CD3+ cells/kg body weight, we observed an overall increase in the CD8+ and CD56+ T cell counts. We determined significant changes between day − 1 compared to day + 1 and day + 7 in memory and activated CD8+ subsets and CD56+ T cells. Applying a higher dose of DLI (5 × 10<sup>6</sup> CD3+ cells/kg) led to a significant increase in the overall counts and subsets of CD8+, CD4+, and NK cells. In conclusion, serial immune phenotyping in the peripheral blood of DLI recipients revealed significant changes in immune effector cells, in particular for various CD8+ T cell subtypes, indicating proliferation and differentiation. |
| first_indexed | 2024-03-10T04:00:19Z |
| format | Article |
| id | doaj.art-4145abed967f49bfbbd13e4707b31022 |
| institution | Directory Open Access Journal |
| issn | 2673-6357 |
| language | English |
| last_indexed | 2024-03-10T04:00:19Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hemato |
| spelling | doaj.art-4145abed967f49bfbbd13e4707b310222023-11-23T08:35:39ZengMDPI AGHemato2673-63572021-11-012469270210.3390/hemato2040046Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell TransplantationAnn-Kristin Schmaelter0Johanna Waidhauser1Dina Kaiser2Tatjana Lenskaja3Stefanie Gruetzner4Rainer Claus5Martin Trepel6Christoph Schmid7Andreas Rank8Department of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyDepartment of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyMedical Faculty, Ludwig Maximilian University of Munich, 80539 Munich, GermanyInstitute of Transfusion Medicine and Hemostasis, University Hospital Augsburg, 86156 Augsburg, GermanyInstitute of Transfusion Medicine and Hemostasis, University Hospital Augsburg, 86156 Augsburg, GermanyDepartment of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyDepartment of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyDepartment of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyDepartment of Hematology and Oncology, University Hospital Augsburg, 86156 Augsburg, GermanyDonor lymphocyte infusion (DLI) after allogeneic stem cell transplantation (alloSCT) is an established method to enhance the Graft-versus-Leukemia (GvL) effect. However, alterations of cellular subsets in the peripheral blood of DLI recipients have not been studied. We investigated the changes in lymphocyte subpopulations in 16 patients receiving DLI after successful alloSCT. Up to three DLIs were applied in escalating doses, prophylactically for relapse prevention in high-risk disease (<i>n</i> = 5), preemptively for mixed chimerism and/or a molecular relapse/persistence (<i>n</i> = 8), or as part of treatment for hematological relapse (<i>n</i> = 3). We used immunophenotyping to measure the absolute numbers of CD4+, CD8+, NK, and CD56+ T cells and their respective subsets in patients’ peripheral blood one day before DLI (d-1) and compared the results at day + 1 and + 7 post DLI to the values before DLI. After the administration of 1 × 10<sup>6</sup> CD3+ cells/kg body weight, we observed an overall increase in the CD8+ and CD56+ T cell counts. We determined significant changes between day − 1 compared to day + 1 and day + 7 in memory and activated CD8+ subsets and CD56+ T cells. Applying a higher dose of DLI (5 × 10<sup>6</sup> CD3+ cells/kg) led to a significant increase in the overall counts and subsets of CD8+, CD4+, and NK cells. In conclusion, serial immune phenotyping in the peripheral blood of DLI recipients revealed significant changes in immune effector cells, in particular for various CD8+ T cell subtypes, indicating proliferation and differentiation.https://www.mdpi.com/2673-6357/2/4/46donor lymphocyte infusionT lymphocytesallogeneic stem cell transplantationgraft-versus-leukemia effectgraft-versus-host diseaseimmunophenotyping |
| spellingShingle | Ann-Kristin Schmaelter Johanna Waidhauser Dina Kaiser Tatjana Lenskaja Stefanie Gruetzner Rainer Claus Martin Trepel Christoph Schmid Andreas Rank Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation Hemato donor lymphocyte infusion T lymphocytes allogeneic stem cell transplantation graft-versus-leukemia effect graft-versus-host disease immunophenotyping |
| title | Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation |
| title_full | Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation |
| title_fullStr | Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation |
| title_full_unstemmed | Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation |
| title_short | Alterations of Peripheral Blood T Cell Subsets following Donor Lymphocyte Infusion in Patients after Allogeneic Stem Cell Transplantation |
| title_sort | alterations of peripheral blood t cell subsets following donor lymphocyte infusion in patients after allogeneic stem cell transplantation |
| topic | donor lymphocyte infusion T lymphocytes allogeneic stem cell transplantation graft-versus-leukemia effect graft-versus-host disease immunophenotyping |
| url | https://www.mdpi.com/2673-6357/2/4/46 |
| work_keys_str_mv | AT annkristinschmaelter alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT johannawaidhauser alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT dinakaiser alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT tatjanalenskaja alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT stefaniegruetzner alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT rainerclaus alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT martintrepel alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT christophschmid alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation AT andreasrank alterationsofperipheralbloodtcellsubsetsfollowingdonorlymphocyteinfusioninpatientsafterallogeneicstemcelltransplantation |