General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches
Abstract A general protocol is developed to obtain D‐glucosamine from three widely available biomass residues: shrimp shells, cicada sloughs, and cockroaches. The protocol includes three steps: (1) demineralization, (2) deproteinization, and (3) chitin hydrolysis. This simple, general protocol opens...
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| Format: | Article |
| Language: | English |
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Wiley
2018-11-01
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| Series: | Global Challenges |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/gch2.201800046 |
| _version_ | 1797743642898595840 |
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| author | Diego L. Bertuzzi Tiago B. Becher Naylil M. R. Capreti Julio Amorim Igor D. Jurberg Jackson D. Megiatto Jr. Catia Ornelas |
| author_facet | Diego L. Bertuzzi Tiago B. Becher Naylil M. R. Capreti Julio Amorim Igor D. Jurberg Jackson D. Megiatto Jr. Catia Ornelas |
| author_sort | Diego L. Bertuzzi |
| collection | DOAJ |
| description | Abstract A general protocol is developed to obtain D‐glucosamine from three widely available biomass residues: shrimp shells, cicada sloughs, and cockroaches. The protocol includes three steps: (1) demineralization, (2) deproteinization, and (3) chitin hydrolysis. This simple, general protocol opens the door to obtain an invaluable nitrogen‐containing compound from three biomass residues, and it can potentially be applied to other chitin sources. White needle‐like crystals of pure D‐glucosamine are obtained in all cases upon purification by crystallization. Characterization data (NMR, IR, and mass spectrometry) of D‐glucosamine obtained from the three chitin sources are similar and confirm its high purity. NMR investigation demonstrates that D‐glucosamine is obtained mainly as the α‐anomer, which undergoes mutarotation in aqueous solution achieving equilibrium after 440 min, in which the anomeric glucosamine distribution is 60% α‐anomer and 40% β‐anomer. |
| first_indexed | 2024-03-12T14:59:20Z |
| format | Article |
| id | doaj.art-41474cbda9094705967176cbba84ee05 |
| institution | Directory Open Access Journal |
| issn | 2056-6646 |
| language | English |
| last_indexed | 2024-03-12T14:59:20Z |
| publishDate | 2018-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Global Challenges |
| spelling | doaj.art-41474cbda9094705967176cbba84ee052023-08-14T09:40:50ZengWileyGlobal Challenges2056-66462018-11-01211n/an/a10.1002/gch2.201800046General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and CockroachesDiego L. Bertuzzi0Tiago B. Becher1Naylil M. R. Capreti2Julio Amorim3Igor D. Jurberg4Jackson D. Megiatto Jr.5Catia Ornelas6Institute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilInstitute of Chemistry University of Campinas – Unicamp Campinas SP 13083‐970 BrazilAbstract A general protocol is developed to obtain D‐glucosamine from three widely available biomass residues: shrimp shells, cicada sloughs, and cockroaches. The protocol includes three steps: (1) demineralization, (2) deproteinization, and (3) chitin hydrolysis. This simple, general protocol opens the door to obtain an invaluable nitrogen‐containing compound from three biomass residues, and it can potentially be applied to other chitin sources. White needle‐like crystals of pure D‐glucosamine are obtained in all cases upon purification by crystallization. Characterization data (NMR, IR, and mass spectrometry) of D‐glucosamine obtained from the three chitin sources are similar and confirm its high purity. NMR investigation demonstrates that D‐glucosamine is obtained mainly as the α‐anomer, which undergoes mutarotation in aqueous solution achieving equilibrium after 440 min, in which the anomeric glucosamine distribution is 60% α‐anomer and 40% β‐anomer.https://doi.org/10.1002/gch2.201800046biomasschitinglucosaminemutarotationresidues |
| spellingShingle | Diego L. Bertuzzi Tiago B. Becher Naylil M. R. Capreti Julio Amorim Igor D. Jurberg Jackson D. Megiatto Jr. Catia Ornelas General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches Global Challenges biomass chitin glucosamine mutarotation residues |
| title | General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches |
| title_full | General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches |
| title_fullStr | General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches |
| title_full_unstemmed | General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches |
| title_short | General Protocol to Obtain D‐Glucosamine from Biomass Residues: Shrimp Shells, Cicada Sloughs and Cockroaches |
| title_sort | general protocol to obtain d glucosamine from biomass residues shrimp shells cicada sloughs and cockroaches |
| topic | biomass chitin glucosamine mutarotation residues |
| url | https://doi.org/10.1002/gch2.201800046 |
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