Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses
ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2017-08-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425&lng=en&tlng=en |
| _version_ | 1828232698376749056 |
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| author | Lamiaa Elsayed Mokhtar Deef |
| author_facet | Lamiaa Elsayed Mokhtar Deef |
| author_sort | Lamiaa Elsayed Mokhtar Deef |
| collection | DOAJ |
| description | ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted white quail (10 bands) and the maximum were in the Japanese quail (18 bands) as measured by SDS-polyacrylamide gel electrophoresis. The Dotted white quail revealed the lowest similarity to the Bobwhite with a coefficient of 0.18 while The similarity coefficients between the Common quail and each one of the other quails were 0.67, 0.62, 0.45 and 0.42 for the Japanese, Panda, Dotted white and the Bobwhite quails, respectively. The results indicate that, PCR-RFLP and protein analyses are good techniques to evaluate genetic characterization and genetic relationship of these quails. |
| first_indexed | 2024-04-12T19:26:10Z |
| format | Article |
| id | doaj.art-4147dab3a1624b41bd2c3338cfbadd0e |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-12T19:26:10Z |
| publishDate | 2017-08-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-4147dab3a1624b41bd2c3338cfbadd0e2022-12-22T03:19:28ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242017-08-0160010.1590/1678-4324-2017160466S1516-89132017000100425Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers AnalysesLamiaa Elsayed Mokhtar DeefABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted white quail (10 bands) and the maximum were in the Japanese quail (18 bands) as measured by SDS-polyacrylamide gel electrophoresis. The Dotted white quail revealed the lowest similarity to the Bobwhite with a coefficient of 0.18 while The similarity coefficients between the Common quail and each one of the other quails were 0.67, 0.62, 0.45 and 0.42 for the Japanese, Panda, Dotted white and the Bobwhite quails, respectively. The results indicate that, PCR-RFLP and protein analyses are good techniques to evaluate genetic characterization and genetic relationship of these quails.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425&lng=en&tlng=enbirdsPCRquailSDS-PAGEGalliformes |
| spellingShingle | Lamiaa Elsayed Mokhtar Deef Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses Brazilian Archives of Biology and Technology birds PCR quail SDS-PAGE Galliformes |
| title | Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses |
| title_full | Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses |
| title_fullStr | Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses |
| title_full_unstemmed | Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses |
| title_short | Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses |
| title_sort | detection of genetic relationship in the tree of life of some quail through molecular markers analyses |
| topic | birds PCR quail SDS-PAGE Galliformes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425&lng=en&tlng=en |
| work_keys_str_mv | AT lamiaaelsayedmokhtardeef detectionofgeneticrelationshipinthetreeoflifeofsomequailthroughmolecularmarkersanalyses |