Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit
In the present study, we examined the effects of antihistamine on the up-regulation of H1R mRNA in the nasal mucosa of patients with pollinosis induced by controlled exposure to pollen using an environmental exposure unit. Out of 20 patients, we designated 14 responders, whose levels of H1R mRNA in...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-11-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S134786131500211X |
| _version_ | 1811263283854835712 |
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| author | Yoshiaki Kitamura Hideyuki Nakagawa Tatsuya Fujii Takema Sakoda Tadao Enomoto Hiroyuki Mizuguchi Hiroyuki Fukui Noriaki Takeda |
| author_facet | Yoshiaki Kitamura Hideyuki Nakagawa Tatsuya Fujii Takema Sakoda Tadao Enomoto Hiroyuki Mizuguchi Hiroyuki Fukui Noriaki Takeda |
| author_sort | Yoshiaki Kitamura |
| collection | DOAJ |
| description | In the present study, we examined the effects of antihistamine on the up-regulation of H1R mRNA in the nasal mucosa of patients with pollinosis induced by controlled exposure to pollen using an environmental exposure unit. Out of 20 patients, we designated 14 responders, whose levels of H1R mRNA in the nasal mucosa were increased after the first pollen exposure and excluded 6 non-responders. Accordingly, the first exposure to pollen without treatment significantly induced both nasal symptoms and the up-regulation of H1R mRNA in the nasal mucosa of the responders. Subsequently, prophylactic administration of antihistamine prior to the second pollen exposure significantly inhibited both of the above effects in the responders. Moreover, the nasal expression of H1R mRNA before the second pollen exposure in the responders pretreated with antihistamine was significantly decreased, as compared with that before the first pollen exposure without treatment. These findings suggest that antihistamines suppressed histamine-induced transcriptional activation of H1R gene in the nasal mucosa, in addition to their blocking effect against histamine on H1R, resulting in a decrease of nasal symptoms. These findings further suggest that by their inverse agonistic activity, antihistamines suppress the basal transcription of nasal H1R in the absence of histamine in responders. |
| first_indexed | 2024-04-12T19:42:03Z |
| format | Article |
| id | doaj.art-41483d44679f4588aea21001575b91bd |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T19:42:03Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-41483d44679f4588aea21001575b91bd2022-12-22T03:19:03ZengElsevierJournal of Pharmacological Sciences1347-86132015-11-01129318318710.1016/j.jphs.2015.10.007Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unitYoshiaki Kitamura0Hideyuki Nakagawa1Tatsuya Fujii2Takema Sakoda3Tadao Enomoto4Hiroyuki Mizuguchi5Hiroyuki Fukui6Noriaki Takeda7Department of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Otolaryngology, Rinku General Medical Center, Osaka, JapanNPO Japan Health Promotion Supporting Network, Wakayama, JapanDepartment of Molecular Pharmacology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Molecular Studies for Incurable Diseases, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Otolaryngology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanIn the present study, we examined the effects of antihistamine on the up-regulation of H1R mRNA in the nasal mucosa of patients with pollinosis induced by controlled exposure to pollen using an environmental exposure unit. Out of 20 patients, we designated 14 responders, whose levels of H1R mRNA in the nasal mucosa were increased after the first pollen exposure and excluded 6 non-responders. Accordingly, the first exposure to pollen without treatment significantly induced both nasal symptoms and the up-regulation of H1R mRNA in the nasal mucosa of the responders. Subsequently, prophylactic administration of antihistamine prior to the second pollen exposure significantly inhibited both of the above effects in the responders. Moreover, the nasal expression of H1R mRNA before the second pollen exposure in the responders pretreated with antihistamine was significantly decreased, as compared with that before the first pollen exposure without treatment. These findings suggest that antihistamines suppressed histamine-induced transcriptional activation of H1R gene in the nasal mucosa, in addition to their blocking effect against histamine on H1R, resulting in a decrease of nasal symptoms. These findings further suggest that by their inverse agonistic activity, antihistamines suppress the basal transcription of nasal H1R in the absence of histamine in responders.http://www.sciencedirect.com/science/article/pii/S134786131500211XHistamine H1 receptorAntihistaminesAllergic rhinitisCedar pollinosisEnviromental exposure unit |
| spellingShingle | Yoshiaki Kitamura Hideyuki Nakagawa Tatsuya Fujii Takema Sakoda Tadao Enomoto Hiroyuki Mizuguchi Hiroyuki Fukui Noriaki Takeda Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit Journal of Pharmacological Sciences Histamine H1 receptor Antihistamines Allergic rhinitis Cedar pollinosis Enviromental exposure unit |
| title | Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| title_full | Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| title_fullStr | Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| title_full_unstemmed | Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| title_short | Effects of antihistamine on up-regulation of histamine H1 receptor mRNA in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| title_sort | effects of antihistamine on up regulation of histamine h1 receptor mrna in the nasal mucosa of patients with pollinosis induced by controlled cedar pollen challenge in an environmental exposure unit |
| topic | Histamine H1 receptor Antihistamines Allergic rhinitis Cedar pollinosis Enviromental exposure unit |
| url | http://www.sciencedirect.com/science/article/pii/S134786131500211X |
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