6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress
6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic develo...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/8/1315 |
| _version_ | 1827746247363002368 |
|---|---|
| author | Wenjie Yu Yanxia Peng Xinyue Peng Ze Li Chang Liu Liu Yang Yan Gao Shuang Liang Bao Yuan Chengzhen Chen Nam-hyung Kim Hao Jiang Jiabao Zhang |
| author_facet | Wenjie Yu Yanxia Peng Xinyue Peng Ze Li Chang Liu Liu Yang Yan Gao Shuang Liang Bao Yuan Chengzhen Chen Nam-hyung Kim Hao Jiang Jiabao Zhang |
| author_sort | Wenjie Yu |
| collection | DOAJ |
| description | 6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic development, are unclear. This study explored whether 6-gingerol can be used to improve the quality of in vitro-cultured porcine embryos. The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. In addition, 6-gingerol upregulated <i>NANOG</i>, SRY-box transcription factor 2, cytochrome c oxidase subunit II, mechanistic target of rapamycin kinase, and RPTOR independent companion of MTOR complex 2 while downregulating Caspase 3, baculoviral IAP repeat containing 5, autophagy related 12, and Beclin 1. Most importantly, 6-gingerol significantly increased the levels of p-extracellular regulated protein kinase 1/2 while reducing the levels of p-c-Jun N-terminal kinase 1/2/3 and p-p38. These results indicate that 6-gingerol can promote the development of porcine early embryos in vitro. |
| first_indexed | 2024-03-11T05:18:45Z |
| format | Article |
| id | doaj.art-4148a866f9ec476e9055a12a439d2f04 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:18:45Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4148a866f9ec476e9055a12a439d2f042023-11-17T18:00:02ZengMDPI AGAnimals2076-26152023-04-01138131510.3390/ani130813156-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative StressWenjie Yu0Yanxia Peng1Xinyue Peng2Ze Li3Chang Liu4Liu Yang5Yan Gao6Shuang Liang7Bao Yuan8Chengzhen Chen9Nam-hyung Kim10Hao Jiang11Jiabao Zhang12Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaSchool of Grains, Jilin Business and Technology College, Changchun 130507, ChinaTongyu Grassland Management Station, Changchun 137200, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, ChinaDepartment of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, China6-Gingerol, the main active ingredient in ginger, exhibits a variety of biological activities, such as antioxidant, anti-inflammatory, and anticancer activities, and can affect cell development. However, the effects of 6-gingerol on mammalian reproductive processes, especially early embryonic development, are unclear. This study explored whether 6-gingerol can be used to improve the quality of in vitro-cultured porcine embryos. The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. In addition, 6-gingerol upregulated <i>NANOG</i>, SRY-box transcription factor 2, cytochrome c oxidase subunit II, mechanistic target of rapamycin kinase, and RPTOR independent companion of MTOR complex 2 while downregulating Caspase 3, baculoviral IAP repeat containing 5, autophagy related 12, and Beclin 1. Most importantly, 6-gingerol significantly increased the levels of p-extracellular regulated protein kinase 1/2 while reducing the levels of p-c-Jun N-terminal kinase 1/2/3 and p-p38. These results indicate that 6-gingerol can promote the development of porcine early embryos in vitro.https://www.mdpi.com/2076-2615/13/8/13156-gingerolporcine embryoreactive oxidative stressproliferationapoptosisautophagy |
| spellingShingle | Wenjie Yu Yanxia Peng Xinyue Peng Ze Li Chang Liu Liu Yang Yan Gao Shuang Liang Bao Yuan Chengzhen Chen Nam-hyung Kim Hao Jiang Jiabao Zhang 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress Animals 6-gingerol porcine embryo reactive oxidative stress proliferation apoptosis autophagy |
| title | 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress |
| title_full | 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress |
| title_fullStr | 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress |
| title_full_unstemmed | 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress |
| title_short | 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress |
| title_sort | 6 gingerol improves in vitro porcine embryo development by reducing oxidative stress |
| topic | 6-gingerol porcine embryo reactive oxidative stress proliferation apoptosis autophagy |
| url | https://www.mdpi.com/2076-2615/13/8/1315 |
| work_keys_str_mv | AT wenjieyu 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT yanxiapeng 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT xinyuepeng 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT zeli 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT changliu 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT liuyang 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT yangao 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT shuangliang 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT baoyuan 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT chengzhenchen 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT namhyungkim 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT haojiang 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress AT jiabaozhang 6gingerolimprovesinvitroporcineembryodevelopmentbyreducingoxidativestress |