Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha
BEige and Chediak–Higashi domain containing proteins (BDCPs) have been described to function in membrane-dependent processes in eukaryotes. This role was also observed for the BDCP SPIRRIG (SPI) in the model plant Arabidopsis thaliana in the context of cell morphogenesis. Additionally, AtSPI was fou...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.915268/full |
| _version_ | 1798000512386203648 |
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| author | Eva Koebke Lisa Stephan Markus G. Stetter Martin Hülskamp |
| author_facet | Eva Koebke Lisa Stephan Markus G. Stetter Martin Hülskamp |
| author_sort | Eva Koebke |
| collection | DOAJ |
| description | BEige and Chediak–Higashi domain containing proteins (BDCPs) have been described to function in membrane-dependent processes in eukaryotes. This role was also observed for the BDCP SPIRRIG (SPI) in the model plant Arabidopsis thaliana in the context of cell morphogenesis. Additionally, AtSPI was found to control salt stress resistance by mediating mRNA stability and salt stress-dependent processing body formation. In this work, we utilize an evolutionarily comparative approach to unravel conserved, basal BDCP functions in the liverwort Marchantia polymorpha. Our phenotypic and physiological analyses show that MpSPI is involved in cell morphogenesis and salt resistance regulation, indicating that both functions are evolutionarily conserved between the two species. Co-localization was found with endosomal and P-body markers, suggesting links to membrane-dependent processes and mRNA metabolism. Finally, we present transcriptomics data showing that AtSPI and MpSPI regulate orthologous genes in A. thaliana and M. polymorpha. |
| first_indexed | 2024-04-11T11:21:28Z |
| format | Article |
| id | doaj.art-414bffa62df5458586df3a8240e668ac |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T11:21:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-414bffa62df5458586df3a8240e668ac2022-12-22T04:26:59ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.915268915268Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorphaEva KoebkeLisa StephanMarkus G. StetterMartin HülskampBEige and Chediak–Higashi domain containing proteins (BDCPs) have been described to function in membrane-dependent processes in eukaryotes. This role was also observed for the BDCP SPIRRIG (SPI) in the model plant Arabidopsis thaliana in the context of cell morphogenesis. Additionally, AtSPI was found to control salt stress resistance by mediating mRNA stability and salt stress-dependent processing body formation. In this work, we utilize an evolutionarily comparative approach to unravel conserved, basal BDCP functions in the liverwort Marchantia polymorpha. Our phenotypic and physiological analyses show that MpSPI is involved in cell morphogenesis and salt resistance regulation, indicating that both functions are evolutionarily conserved between the two species. Co-localization was found with endosomal and P-body markers, suggesting links to membrane-dependent processes and mRNA metabolism. Finally, we present transcriptomics data showing that AtSPI and MpSPI regulate orthologous genes in A. thaliana and M. polymorpha.https://www.frontiersin.org/articles/10.3389/fpls.2022.915268/fullSPIRRIGMarchantia polymorphaBEACH domain proteinsalt responsemembrane trafficking |
| spellingShingle | Eva Koebke Lisa Stephan Markus G. Stetter Martin Hülskamp Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha Frontiers in Plant Science SPIRRIG Marchantia polymorpha BEACH domain protein salt response membrane trafficking |
| title | Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha |
| title_full | Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha |
| title_fullStr | Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha |
| title_full_unstemmed | Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha |
| title_short | Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha |
| title_sort | functional analysis of the beige and chediak higashi domain gene mpspirrig in marchantia polymorpha |
| topic | SPIRRIG Marchantia polymorpha BEACH domain protein salt response membrane trafficking |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.915268/full |
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