Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery
Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Asian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1818087623000831 |
| _version_ | 1827577731967090688 |
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| author | Liangwei Si Xiong Guo Hriday Bera Yang Chen Fangfang Xiu Peixin Liu Chunwei Zhao Yasir Faraz Abbasi Xing Tang Vito Foderà Dongmei Cun Mingshi Yang |
| author_facet | Liangwei Si Xiong Guo Hriday Bera Yang Chen Fangfang Xiu Peixin Liu Chunwei Zhao Yasir Faraz Abbasi Xing Tang Vito Foderà Dongmei Cun Mingshi Yang |
| author_sort | Liangwei Si |
| collection | DOAJ |
| description | Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibrous scaffolds (ENs) as compared to other regenerative protein scaffolds were established. Bovine serum albumin (BSA), collagen type I (COL), lysozyme (LZM) and ALA were separately blended with poly(ε-caprolactone) (PCL) to fabricate four different composite ENs (LZM/PCL, BSA/PCL, COL/PCL and ALA/PCL ENs). The hydrophilic composite scaffolds exhibited an enhanced wettability and variable mechanical properties. The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs. As compared to PCL ENs and other composite scaffolds, the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen spongeⓇ on third-degree burn model. The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites. Collectively, this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds. |
| first_indexed | 2024-03-08T21:30:59Z |
| format | Article |
| id | doaj.art-414fe62f4d744bab9afd06fdd70f23c5 |
| institution | Directory Open Access Journal |
| issn | 1818-0876 |
| language | English |
| last_indexed | 2024-03-08T21:30:59Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-414fe62f4d744bab9afd06fdd70f23c52023-12-21T07:29:58ZengElsevierAsian Journal of Pharmaceutical Sciences1818-08762023-11-01186100856Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recoveryLiangwei Si0Xiong Guo1Hriday Bera2Yang Chen3Fangfang Xiu4Peixin Liu5Chunwei Zhao6Yasir Faraz Abbasi7Xing Tang8Vito Foderà9Dongmei Cun10Mingshi Yang11Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, ChinaWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China; Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur 713206, India; Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen O, DenmarkCorresponding authors.; Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China; Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur 713206, India; Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen O, DenmarkWuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors.Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China; Corresponding authors.Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibrous scaffolds (ENs) as compared to other regenerative protein scaffolds were established. Bovine serum albumin (BSA), collagen type I (COL), lysozyme (LZM) and ALA were separately blended with poly(ε-caprolactone) (PCL) to fabricate four different composite ENs (LZM/PCL, BSA/PCL, COL/PCL and ALA/PCL ENs). The hydrophilic composite scaffolds exhibited an enhanced wettability and variable mechanical properties. The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs. As compared to PCL ENs and other composite scaffolds, the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen spongeⓇ on third-degree burn model. The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites. Collectively, this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds.http://www.sciencedirect.com/science/article/pii/S1818087623000831Regenerative proteinsα-lactalbuminSerotoninElectrospinningNanofibrous dressingThird-degree burn |
| spellingShingle | Liangwei Si Xiong Guo Hriday Bera Yang Chen Fangfang Xiu Peixin Liu Chunwei Zhao Yasir Faraz Abbasi Xing Tang Vito Foderà Dongmei Cun Mingshi Yang Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery Asian Journal of Pharmaceutical Sciences Regenerative proteins α-lactalbumin Serotonin Electrospinning Nanofibrous dressing Third-degree burn |
| title | Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery |
| title_full | Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery |
| title_fullStr | Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery |
| title_full_unstemmed | Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery |
| title_short | Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery |
| title_sort | unleashing the healing potential exploring next generation regenerative protein nanoscaffolds for burn wound recovery |
| topic | Regenerative proteins α-lactalbumin Serotonin Electrospinning Nanofibrous dressing Third-degree burn |
| url | http://www.sciencedirect.com/science/article/pii/S1818087623000831 |
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