Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis
Mucus secreted by goblet cells (GCs) may play an important role in intestinal transit function. Our previous study found that Piezo1 protein is essential for GC function; however, the effect of GC Piezo1 on intestinal transit function is unclear. Our study aimed to investigate the effect of Piezo1 i...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/18/14377 |
| _version_ | 1797579572588314624 |
|---|---|
| author | Feifei Fang Ying Liu Yilin Xiong Xueyan Li Gangping Li Yudong Jiang Xiaohua Hou Jun Song |
| author_facet | Feifei Fang Ying Liu Yilin Xiong Xueyan Li Gangping Li Yudong Jiang Xiaohua Hou Jun Song |
| author_sort | Feifei Fang |
| collection | DOAJ |
| description | Mucus secreted by goblet cells (GCs) may play an important role in intestinal transit function. Our previous study found that Piezo1 protein is essential for GC function; however, the effect of GC Piezo1 on intestinal transit function is unclear. Our study aimed to investigate the effect of Piezo1 in GCs on intestinal transit and the potential mechanism. We compared intestinal mucus, fecal form, intestinal transit time, intestinal epithelial cell composition, and stem cell function in WT and GC-specific Piezo1-deficient (Piezo1<sup>ΔGC</sup>) mice. Our results revealed a correlation between mucus and intestinal transit: the less mucus there was, the slower the intestinal transit. Piezo1 deficiency in GCs led to decreased mucus synthesis and also disrupted the ecological niche of colon stem cells (CSCs). Through organoid culture, we found that the capacity of proliferation and differentiation in Piezo1<sup>ΔGC</sup> mouse CSCs was significantly decreased, which also led to a reduced source of GCs. Further studies found that the reduced Wnt and Notch signals in colon crypts might be the potential mechanism. These results indicated the importance of GC Piezo1 in intestinal transit function, which acts by maintaining the homeostasis of intestinal epithelial cells and mucus. |
| first_indexed | 2024-03-10T22:39:03Z |
| format | Article |
| id | doaj.art-41504df3cbb847de9e40ea43c40970c8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:39:03Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-41504df3cbb847de9e40ea43c40970c82023-11-19T11:11:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181437710.3390/ijms241814377Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial HomeostasisFeifei Fang0Ying Liu1Yilin Xiong2Xueyan Li3Gangping Li4Yudong Jiang5Xiaohua Hou6Jun Song7Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaMucus secreted by goblet cells (GCs) may play an important role in intestinal transit function. Our previous study found that Piezo1 protein is essential for GC function; however, the effect of GC Piezo1 on intestinal transit function is unclear. Our study aimed to investigate the effect of Piezo1 in GCs on intestinal transit and the potential mechanism. We compared intestinal mucus, fecal form, intestinal transit time, intestinal epithelial cell composition, and stem cell function in WT and GC-specific Piezo1-deficient (Piezo1<sup>ΔGC</sup>) mice. Our results revealed a correlation between mucus and intestinal transit: the less mucus there was, the slower the intestinal transit. Piezo1 deficiency in GCs led to decreased mucus synthesis and also disrupted the ecological niche of colon stem cells (CSCs). Through organoid culture, we found that the capacity of proliferation and differentiation in Piezo1<sup>ΔGC</sup> mouse CSCs was significantly decreased, which also led to a reduced source of GCs. Further studies found that the reduced Wnt and Notch signals in colon crypts might be the potential mechanism. These results indicated the importance of GC Piezo1 in intestinal transit function, which acts by maintaining the homeostasis of intestinal epithelial cells and mucus.https://www.mdpi.com/1422-0067/24/18/14377Piezo1intestinal transitgoblet cellcolon stem cellintestinal epithelial homeostasis |
| spellingShingle | Feifei Fang Ying Liu Yilin Xiong Xueyan Li Gangping Li Yudong Jiang Xiaohua Hou Jun Song Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis International Journal of Molecular Sciences Piezo1 intestinal transit goblet cell colon stem cell intestinal epithelial homeostasis |
| title | Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis |
| title_full | Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis |
| title_fullStr | Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis |
| title_full_unstemmed | Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis |
| title_short | Slowed Intestinal Transit Induced by Less Mucus in Intestinal Goblet Cell Piezo1-Deficient Mice through Impaired Epithelial Homeostasis |
| title_sort | slowed intestinal transit induced by less mucus in intestinal goblet cell piezo1 deficient mice through impaired epithelial homeostasis |
| topic | Piezo1 intestinal transit goblet cell colon stem cell intestinal epithelial homeostasis |
| url | https://www.mdpi.com/1422-0067/24/18/14377 |
| work_keys_str_mv | AT feifeifang slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT yingliu slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT yilinxiong slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT xueyanli slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT gangpingli slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT yudongjiang slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT xiaohuahou slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis AT junsong slowedintestinaltransitinducedbylessmucusinintestinalgobletcellpiezo1deficientmicethroughimpairedepithelialhomeostasis |