Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides
Dipsacus asperoides is a traditional medicinal herb widely used in inflammation and fracture in Asia. Triterpenoid saponins from D. asperoides are the main composition with pharmacological activity. However, the biosynthesis pathway of triterpenoid saponins has not been completely resolved in D. asp...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1134352/full |
| _version_ | 1811167105853161472 |
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| author | Jie Pan Jie Pan Chaokang Huang Chaokang Huang Weilin Yao Weilin Yao Tengfei Niu Tengfei Niu Xiaolin Yang Xiaolin Yang Xiaolin Yang Rufeng Wang Rufeng Wang Rufeng Wang Rufeng Wang |
| author_facet | Jie Pan Jie Pan Chaokang Huang Chaokang Huang Weilin Yao Weilin Yao Tengfei Niu Tengfei Niu Xiaolin Yang Xiaolin Yang Xiaolin Yang Rufeng Wang Rufeng Wang Rufeng Wang Rufeng Wang |
| author_sort | Jie Pan |
| collection | DOAJ |
| description | Dipsacus asperoides is a traditional medicinal herb widely used in inflammation and fracture in Asia. Triterpenoid saponins from D. asperoides are the main composition with pharmacological activity. However, the biosynthesis pathway of triterpenoid saponins has not been completely resolved in D. asperoides. Here, the types and contents of triterpenoid saponins were discovered with different distributions in five tissues (root, leaf, flower, stem, and fibrous root tissue) from D. asperoides by UPLC-Q-TOF-MS analysis. The discrepancy between five tissues in D. asperoides at the transcriptional level was studied by combining single-molecule real-time sequencing and next- generation sequencing. Meanwhile, key genes involved in the biosynthesis of saponin were further verified by proteomics. In MEP and MVA pathways, 48 differentially expressed genes were identified through co-expression analysis of transcriptome and saponin contents, including two isopentenyl pyrophosphate isomerase and two 2,3-oxidosqualene β-amyrin cyclase, etc. In the analysis of WGCNA, 6 cytochrome P450s and 24 UDP- glycosyltransferases related to the biosynthesis of triterpenoid saponins were discovered with high transcriptome expression. This study will provide profound insights to demonstrate essential genes in the biosynthesis pathway of saponins in D. asperoides and support for the biosynthetic of natural active ingredients in the future. |
| first_indexed | 2024-04-10T16:03:29Z |
| format | Article |
| id | doaj.art-41519053747145f98dadd16cc0ac17e3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T16:03:29Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-41519053747145f98dadd16cc0ac17e32023-02-10T06:46:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11343521134352Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoidesJie Pan0Jie Pan1Chaokang Huang2Chaokang Huang3Weilin Yao4Weilin Yao5Tengfei Niu6Tengfei Niu7Xiaolin Yang8Xiaolin Yang9Xiaolin Yang10Rufeng Wang11Rufeng Wang12Rufeng Wang13Rufeng Wang14Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai R&D Center for Standardization of Chinese Medicines, Shanghai, ChinaInstitute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaThe SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaShanghai R&D Center for Standardization of Chinese Medicines, Shanghai, ChinaThe MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDipsacus asperoides is a traditional medicinal herb widely used in inflammation and fracture in Asia. Triterpenoid saponins from D. asperoides are the main composition with pharmacological activity. However, the biosynthesis pathway of triterpenoid saponins has not been completely resolved in D. asperoides. Here, the types and contents of triterpenoid saponins were discovered with different distributions in five tissues (root, leaf, flower, stem, and fibrous root tissue) from D. asperoides by UPLC-Q-TOF-MS analysis. The discrepancy between five tissues in D. asperoides at the transcriptional level was studied by combining single-molecule real-time sequencing and next- generation sequencing. Meanwhile, key genes involved in the biosynthesis of saponin were further verified by proteomics. In MEP and MVA pathways, 48 differentially expressed genes were identified through co-expression analysis of transcriptome and saponin contents, including two isopentenyl pyrophosphate isomerase and two 2,3-oxidosqualene β-amyrin cyclase, etc. In the analysis of WGCNA, 6 cytochrome P450s and 24 UDP- glycosyltransferases related to the biosynthesis of triterpenoid saponins were discovered with high transcriptome expression. This study will provide profound insights to demonstrate essential genes in the biosynthesis pathway of saponins in D. asperoides and support for the biosynthetic of natural active ingredients in the future.https://www.frontiersin.org/articles/10.3389/fpls.2023.1134352/fullDipsacus asperoidessaponin distributionbiosynthesistranscriptomeproteomics |
| spellingShingle | Jie Pan Jie Pan Chaokang Huang Chaokang Huang Weilin Yao Weilin Yao Tengfei Niu Tengfei Niu Xiaolin Yang Xiaolin Yang Xiaolin Yang Rufeng Wang Rufeng Wang Rufeng Wang Rufeng Wang Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides Frontiers in Plant Science Dipsacus asperoides saponin distribution biosynthesis transcriptome proteomics |
| title | Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides |
| title_full | Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides |
| title_fullStr | Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides |
| title_full_unstemmed | Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides |
| title_short | Full-length transcriptome, proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in Dipsacus asperoides |
| title_sort | full length transcriptome proteomics and metabolite analysis reveal candidate genes involved triterpenoid saponin biosynthesis in dipsacus asperoides |
| topic | Dipsacus asperoides saponin distribution biosynthesis transcriptome proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1134352/full |
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