Understanding the molecular mechanisms underlying graft success in grapevine
Abstract Background Grafting is an intensive commercial practice required to protect the European grapevine against the Phylloxera pest. Rootstocks resistant to this pest are hybrids of American vine species with different levels of compatibility with European Vitis vinifera varieties. Aiming to und...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1967-8 |
| _version_ | 1818845897453207552 |
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| author | M. Assunção C. Santos J. Brazão J. E. Eiras-Dias P. Fevereiro |
| author_facet | M. Assunção C. Santos J. Brazão J. E. Eiras-Dias P. Fevereiro |
| author_sort | M. Assunção |
| collection | DOAJ |
| description | Abstract Background Grafting is an intensive commercial practice required to protect the European grapevine against the Phylloxera pest. Rootstocks resistant to this pest are hybrids of American vine species with different levels of compatibility with European Vitis vinifera varieties. Aiming to understand what drives grafting compatibility in grapevine, a transcriptomic approach was used to search for master regulators of graft success. Two scion/rootstock combinations, with different levels of compatibility, were compared in a nursery-grafting context at two stages, at 21 and 80 days after grafting. Results In the most compatible combination, an earlier and higher expression of genes signaling the metabolic and hormonal pathways as well as a reduced expression of genes of the phenolic metabolism and of the oxidative stress response was observed. At 80 days after grafting a higher expression of transcription factors regulating vascular maintenance, differentiation and proliferation was obtained in the most compatible combination. Moreover, lower expression levels of microRNAs potentially targeting important transcription factors related to plant development was observed in the more compatible combination when compared to the less compatible one. Conclusion In this context, a set of regulators was selected as potential expression markers for early prediction of a compatible grafting. |
| first_indexed | 2024-12-19T05:36:57Z |
| format | Article |
| id | doaj.art-41569d49f73443bcb937da8973de242b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T05:36:57Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-41569d49f73443bcb937da8973de242b2022-12-21T20:34:06ZengBMCBMC Plant Biology1471-22292019-09-0119111710.1186/s12870-019-1967-8Understanding the molecular mechanisms underlying graft success in grapevineM. Assunção0C. Santos1J. Brazão2J. E. Eiras-Dias3P. Fevereiro4Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de LisboaGenetics and Genomics of Plant Complex Traits (PlantX) Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de LisboaInstituto Nacional de Investigação Agrária e Veterinária (Biotechnology and Genetic Genetic Resources Unit) INIAV-Dois PortosInstituto Nacional de Investigação Agrária e Veterinária (Biotechnology and Genetic Genetic Resources Unit) INIAV-Dois PortosPlant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Universidade Nova de LisboaAbstract Background Grafting is an intensive commercial practice required to protect the European grapevine against the Phylloxera pest. Rootstocks resistant to this pest are hybrids of American vine species with different levels of compatibility with European Vitis vinifera varieties. Aiming to understand what drives grafting compatibility in grapevine, a transcriptomic approach was used to search for master regulators of graft success. Two scion/rootstock combinations, with different levels of compatibility, were compared in a nursery-grafting context at two stages, at 21 and 80 days after grafting. Results In the most compatible combination, an earlier and higher expression of genes signaling the metabolic and hormonal pathways as well as a reduced expression of genes of the phenolic metabolism and of the oxidative stress response was observed. At 80 days after grafting a higher expression of transcription factors regulating vascular maintenance, differentiation and proliferation was obtained in the most compatible combination. Moreover, lower expression levels of microRNAs potentially targeting important transcription factors related to plant development was observed in the more compatible combination when compared to the less compatible one. Conclusion In this context, a set of regulators was selected as potential expression markers for early prediction of a compatible grafting.http://link.springer.com/article/10.1186/s12870-019-1967-8GrapevineGraftingGraft compatibilityMolecular mechanism of graftingVascular differentiationTranscriptional regulation of grafting |
| spellingShingle | M. Assunção C. Santos J. Brazão J. E. Eiras-Dias P. Fevereiro Understanding the molecular mechanisms underlying graft success in grapevine BMC Plant Biology Grapevine Grafting Graft compatibility Molecular mechanism of grafting Vascular differentiation Transcriptional regulation of grafting |
| title | Understanding the molecular mechanisms underlying graft success in grapevine |
| title_full | Understanding the molecular mechanisms underlying graft success in grapevine |
| title_fullStr | Understanding the molecular mechanisms underlying graft success in grapevine |
| title_full_unstemmed | Understanding the molecular mechanisms underlying graft success in grapevine |
| title_short | Understanding the molecular mechanisms underlying graft success in grapevine |
| title_sort | understanding the molecular mechanisms underlying graft success in grapevine |
| topic | Grapevine Grafting Graft compatibility Molecular mechanism of grafting Vascular differentiation Transcriptional regulation of grafting |
| url | http://link.springer.com/article/10.1186/s12870-019-1967-8 |
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