Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice
Homeobox A10 (HOXA10) is a well-known transcription factor that plays an important role in directing endometrial differentiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXA10 may be associated with litter size. To study the effects of the po...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915611698 |
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| author | Rui-yi LIN Di WU Chang-zhi ZHAO Shang-shang CHEN Qian XIAO Xin-yun LI Shu-hong ZHAO |
| author_facet | Rui-yi LIN Di WU Chang-zhi ZHAO Shang-shang CHEN Qian XIAO Xin-yun LI Shu-hong ZHAO |
| author_sort | Rui-yi LIN |
| collection | DOAJ |
| description | Homeobox A10 (HOXA10) is a well-known transcription factor that plays an important role in directing endometrial differentiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXA10 may be associated with litter size. To study the effects of the porcine HOXA10 promoter fragment on the expression of HOXA10 gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXA10 in the endometrium. There was no difference in the expression level of HOXA10 between transgenic and wild-type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXA10 was significantly increased in transgenic mice compared with that of wild-type mice. Furthermore, the litter size of transgenic females was larger than that of wild-type females (7.02±1.73 vs. 6.48±1.85; P=0.14). Moreover, the difference of litter size was greater in the later parities (7.33±1.62 vs. 6.37±2.02; P=0.08) compared with the first parity (6.76±1.81 vs. 6.61±1.67; P=0.77) between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXA10 and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXA10 to alter litter size. |
| first_indexed | 2024-12-14T20:24:38Z |
| format | Article |
| id | doaj.art-415a6c1b514a43ea86a4546475eb3e56 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-14T20:24:38Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-415a6c1b514a43ea86a4546475eb3e562022-12-21T22:48:39ZengElsevierJournal of Integrative Agriculture2095-31192016-06-0115613381344Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic miceRui-yi LIN0Di WU1Chang-zhi ZHAO2Shang-shang CHEN3Qian XIAO4Xin-yun LI5Shu-hong ZHAO6Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaKey Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R.China; Correspondence ZHAO Shu-hong, Tel: +86-27-87387480, Fax: +86-27-87280408Homeobox A10 (HOXA10) is a well-known transcription factor that plays an important role in directing endometrial differentiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXA10 may be associated with litter size. To study the effects of the porcine HOXA10 promoter fragment on the expression of HOXA10 gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXA10 in the endometrium. There was no difference in the expression level of HOXA10 between transgenic and wild-type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXA10 was significantly increased in transgenic mice compared with that of wild-type mice. Furthermore, the litter size of transgenic females was larger than that of wild-type females (7.02±1.73 vs. 6.48±1.85; P=0.14). Moreover, the difference of litter size was greater in the later parities (7.33±1.62 vs. 6.37±2.02; P=0.08) compared with the first parity (6.76±1.81 vs. 6.61±1.67; P=0.77) between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXA10 and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXA10 to alter litter size.http://www.sciencedirect.com/science/article/pii/S2095311915611698porcineHOXA10transgenic miceendometrium |
| spellingShingle | Rui-yi LIN Di WU Chang-zhi ZHAO Shang-shang CHEN Qian XIAO Xin-yun LI Shu-hong ZHAO Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice Journal of Integrative Agriculture porcine HOXA10 transgenic mice endometrium |
| title | Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice |
| title_full | Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice |
| title_fullStr | Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice |
| title_full_unstemmed | Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice |
| title_short | Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice |
| title_sort | inducible overexpression of porcine homeobox a10 in the endometrium of transgenic mice |
| topic | porcine HOXA10 transgenic mice endometrium |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915611698 |
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