Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar
Salicylic acid (SA) is generally considered to be a critical signal transduction factor in plant defenses against pathogens. It could be converted to methyl salicylate (MeSA) for remote signals by salicylic acid methyltransferase (SAMT) and converted back to SA by SA-binding protein 2 (SABP2). In or...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.973305/full |
| _version_ | 1811246031576236032 |
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| author | Huixia Dong Huixia Dong Wei Zhang Wei Zhang Yongxia Li Yongxia Li Yuqian Feng Yuqian Feng Xuan Wang Xuan Wang Zhenkai Liu Zhenkai Liu Dongzhen Li Dongzhen Li Xiaojian Wen Xiaojian Wen Shuai Ma Xingyao Zhang Xingyao Zhang |
| author_facet | Huixia Dong Huixia Dong Wei Zhang Wei Zhang Yongxia Li Yongxia Li Yuqian Feng Yuqian Feng Xuan Wang Xuan Wang Zhenkai Liu Zhenkai Liu Dongzhen Li Dongzhen Li Xiaojian Wen Xiaojian Wen Shuai Ma Xingyao Zhang Xingyao Zhang |
| author_sort | Huixia Dong |
| collection | DOAJ |
| description | Salicylic acid (SA) is generally considered to be a critical signal transduction factor in plant defenses against pathogens. It could be converted to methyl salicylate (MeSA) for remote signals by salicylic acid methyltransferase (SAMT) and converted back to SA by SA-binding protein 2 (SABP2). In order to verify the function of SAMT in poplar plants, we isolated the full-length cDNA sequence of PagSAMT from 84K poplar and cultivated PagSAMT overexpression lines (OE-2 isolate) to test its role in SA-mediated defenses against the virulent fungal pathogen Botryosphaeria dothidea. Our results showed that after inoculation with B. dothidea, OE-2 significantly increased MeSA content and reduced SA content which is associated with increased expression of SAMT in both infected and uninfected leaves, when compared against the wild type (WT). Additionally, SAMT overexpression plant lines (OE-2) exhibited higher expression of pathogenesis-related genes PR-1 and PR-5, but were still susceptible to B. dothidea suggesting that in poplar SA might be responsible for resistance against this pathogen. This study expands the current understanding of joint regulation of SAMT and SABP2 and the balance between SA and MeSA in poplar responses to pathogen invasion. |
| first_indexed | 2024-04-12T14:47:04Z |
| format | Article |
| id | doaj.art-415c26c825e740d89ef6c042873e1929 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T14:47:04Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-415c26c825e740d89ef6c042873e19292022-12-22T03:28:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.973305973305Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplarHuixia Dong0Huixia Dong1Wei Zhang2Wei Zhang3Yongxia Li4Yongxia Li5Yuqian Feng6Yuqian Feng7Xuan Wang8Xuan Wang9Zhenkai Liu10Zhenkai Liu11Dongzhen Li12Dongzhen Li13Xiaojian Wen14Xiaojian Wen15Shuai Ma16Xingyao Zhang17Xingyao Zhang18Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCollege of Life Science, Henan Normal University, Xinxiang, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaResources Management, Chinese Academy of Forestry, Beijing, ChinaKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaSalicylic acid (SA) is generally considered to be a critical signal transduction factor in plant defenses against pathogens. It could be converted to methyl salicylate (MeSA) for remote signals by salicylic acid methyltransferase (SAMT) and converted back to SA by SA-binding protein 2 (SABP2). In order to verify the function of SAMT in poplar plants, we isolated the full-length cDNA sequence of PagSAMT from 84K poplar and cultivated PagSAMT overexpression lines (OE-2 isolate) to test its role in SA-mediated defenses against the virulent fungal pathogen Botryosphaeria dothidea. Our results showed that after inoculation with B. dothidea, OE-2 significantly increased MeSA content and reduced SA content which is associated with increased expression of SAMT in both infected and uninfected leaves, when compared against the wild type (WT). Additionally, SAMT overexpression plant lines (OE-2) exhibited higher expression of pathogenesis-related genes PR-1 and PR-5, but were still susceptible to B. dothidea suggesting that in poplar SA might be responsible for resistance against this pathogen. This study expands the current understanding of joint regulation of SAMT and SABP2 and the balance between SA and MeSA in poplar responses to pathogen invasion.https://www.frontiersin.org/articles/10.3389/fpls.2022.973305/fullpoplarsalicylic acid methyltransferasesalicylic acidmethyl salicylatepathogen resistance |
| spellingShingle | Huixia Dong Huixia Dong Wei Zhang Wei Zhang Yongxia Li Yongxia Li Yuqian Feng Yuqian Feng Xuan Wang Xuan Wang Zhenkai Liu Zhenkai Liu Dongzhen Li Dongzhen Li Xiaojian Wen Xiaojian Wen Shuai Ma Xingyao Zhang Xingyao Zhang Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar Frontiers in Plant Science poplar salicylic acid methyltransferase salicylic acid methyl salicylate pathogen resistance |
| title | Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar |
| title_full | Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar |
| title_fullStr | Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar |
| title_full_unstemmed | Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar |
| title_short | Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar |
| title_sort | overexpression of salicylic acid methyltransferase reduces salicylic acid mediated pathogen resistance in poplar |
| topic | poplar salicylic acid methyltransferase salicylic acid methyl salicylate pathogen resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.973305/full |
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