#170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation
Background and Aims: A 35-year-old patient with secondary infertility underwent oocyte freezing at Alpha IVF in January 2021. She had 13 oocytes retrieved, of which, only 3 were mature and vitrified on Day 0 (Day0-MII) while the remaining 10 immature oocytes were left to mature overnight. On the fol...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2023-12-01
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| Series: | Fertility & Reproduction |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S2661318223744065 |
| _version_ | 1831877995401314304 |
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| author | Siow Shein Chok Mei Wei Lim Chong Seong Tan Colin Soon Soo Lee |
| author_facet | Siow Shein Chok Mei Wei Lim Chong Seong Tan Colin Soon Soo Lee |
| author_sort | Siow Shein Chok |
| collection | DOAJ |
| description | Background and Aims: A 35-year-old patient with secondary infertility underwent oocyte freezing at Alpha IVF in January 2021. She had 13 oocytes retrieved, of which, only 3 were mature and vitrified on Day 0 (Day0-MII) while the remaining 10 immature oocytes were left to mature overnight. On the following day, 8 mature oocytes (Day1-MII) were vitrified. This case report describes a live birth following frozen embryo transfer (FET) of a Day 7 euploid blastocyst derived from vitrified-warmed Day1-MII oocytes. Method: All of the patient’s MII oocytes were vitrified and warmed using Cryotec method (Cryotech, Japan). The oocytes were inseminated using PIEZO-ICSI (Japan) and were cultured up to 7 days. Usable blastocysts (with at least a Gardner’s score of BB) had their trophectoderm biopsied and subsequently vitrified. Biopsied cells were subjected to preimplantation genetic testing-aneuploidy (PGT-A) using next generation sequencing (Ion Torrent, USA). Finally, a euploid blastocyst was warmed and transferred. Results: Of the 11 vitrified MII oocytes, 10 survived post-warmed and 7 fertilized post-insemination: 2 from Day0-MII oocytes; 5 from Day1-MII oocytes. After 7 days of culture, none of the vitrified-warmed Day0-MII oocytes became usable blastocysts. Out of the 5 fertilised Day1-MII oocytes, 2 developed into usable blastocysts (1 day 6 blastocyst; 1 day 7 blastocyst) and were subjected to PGT-A and vitrification. Of the 2 blastocysts analysed, only the day 7 blastocyst was a euploid. Subsequently, this blastocyst was warmed and transferred in June 2022 which led to a clinical pregnancy. In February 2023, the patient underwent an uneventful delivery at 38 weeks of pregnancy. Conclusion: This case report demonstrated that a day 7 blastocyst derived from vitrified-warmed Day1-MII oocytes can result in a healthy live birth. We advocate that the salvaging of immature oocytes on Day 0 should be practiced. |
| first_indexed | 2024-04-24T17:13:39Z |
| format | Article |
| id | doaj.art-415ca908c4594172bfd1ab2d021290c8 |
| institution | Directory Open Access Journal |
| issn | 2661-3182 2661-3174 |
| language | English |
| last_indexed | 2025-03-21T21:24:28Z |
| publishDate | 2023-12-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Fertility & Reproduction |
| spelling | doaj.art-415ca908c4594172bfd1ab2d021290c82024-05-28T17:42:15ZengWorld Scientific PublishingFertility & Reproduction2661-31822661-31742023-12-01050469169110.1142/S2661318223744065#170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed MaturationSiow Shein Chok0Mei Wei Lim1Chong Seong Tan2Colin Soon Soo Lee3Alpha IVF n Women’s Specialists, Petaling Jaya, MalaysiaAlpha IVF n Women’s Specialists, Petaling Jaya, MalaysiaAlpha IVF n Women’s Specialists, Petaling Jaya, MalaysiaAlpha IVF n Women’s Specialists, Petaling Jaya, MalaysiaBackground and Aims: A 35-year-old patient with secondary infertility underwent oocyte freezing at Alpha IVF in January 2021. She had 13 oocytes retrieved, of which, only 3 were mature and vitrified on Day 0 (Day0-MII) while the remaining 10 immature oocytes were left to mature overnight. On the following day, 8 mature oocytes (Day1-MII) were vitrified. This case report describes a live birth following frozen embryo transfer (FET) of a Day 7 euploid blastocyst derived from vitrified-warmed Day1-MII oocytes. Method: All of the patient’s MII oocytes were vitrified and warmed using Cryotec method (Cryotech, Japan). The oocytes were inseminated using PIEZO-ICSI (Japan) and were cultured up to 7 days. Usable blastocysts (with at least a Gardner’s score of BB) had their trophectoderm biopsied and subsequently vitrified. Biopsied cells were subjected to preimplantation genetic testing-aneuploidy (PGT-A) using next generation sequencing (Ion Torrent, USA). Finally, a euploid blastocyst was warmed and transferred. Results: Of the 11 vitrified MII oocytes, 10 survived post-warmed and 7 fertilized post-insemination: 2 from Day0-MII oocytes; 5 from Day1-MII oocytes. After 7 days of culture, none of the vitrified-warmed Day0-MII oocytes became usable blastocysts. Out of the 5 fertilised Day1-MII oocytes, 2 developed into usable blastocysts (1 day 6 blastocyst; 1 day 7 blastocyst) and were subjected to PGT-A and vitrification. Of the 2 blastocysts analysed, only the day 7 blastocyst was a euploid. Subsequently, this blastocyst was warmed and transferred in June 2022 which led to a clinical pregnancy. In February 2023, the patient underwent an uneventful delivery at 38 weeks of pregnancy. Conclusion: This case report demonstrated that a day 7 blastocyst derived from vitrified-warmed Day1-MII oocytes can result in a healthy live birth. We advocate that the salvaging of immature oocytes on Day 0 should be practiced.https://www.worldscientific.com/doi/10.1142/S2661318223744065 |
| spellingShingle | Siow Shein Chok Mei Wei Lim Chong Seong Tan Colin Soon Soo Lee #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation Fertility & Reproduction |
| title | #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation |
| title_full | #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation |
| title_fullStr | #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation |
| title_full_unstemmed | #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation |
| title_short | #170 : Case Report: Live Birth Following Frozen Embryo Transfer of a Day 7 Euploid Blastocyst Derived from Vitrified-Warmed Oocytes with Delayed Maturation |
| title_sort | 170 case report live birth following frozen embryo transfer of a day 7 euploid blastocyst derived from vitrified warmed oocytes with delayed maturation |
| url | https://www.worldscientific.com/doi/10.1142/S2661318223744065 |
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