DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China
The orchid genus Oberonia Lindl., is a taxonomically complex genus characterized by recent species radiations and many closely related species. All Oberonia species are under conservation as listed in the CITES and the IUCN Red List Categories and Criteria. Given its difficulties in taxonomy and con...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01791/full |
| _version_ | 1819267300475273216 |
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| author | Yuling Li Yuling Li Yi Tong Yi Tong Fuwu Xing |
| author_facet | Yuling Li Yuling Li Yi Tong Yi Tong Fuwu Xing |
| author_sort | Yuling Li |
| collection | DOAJ |
| description | The orchid genus Oberonia Lindl., is a taxonomically complex genus characterized by recent species radiations and many closely related species. All Oberonia species are under conservation as listed in the CITES and the IUCN Red List Categories and Criteria. Given its difficulties in taxonomy and conservation status, Oberonia is an excellent model for developing DNA barcodes. Three analytical methods and five DNA barcoding regions (rbcL, matK, trnH-psbA, ITS and ITS2) were evaluated on 127 individuals representing 40 species and 1 variety of Oberonia from China. All the three plastid candidates tested (rbcL, matK and trnH-psbA) have a lower discriminatory power than the nuclear regions (ITS and ITS2), and ITS had the highest resolution rate (82.14%). Two to four combinations of these gene sets were not better than the ITS alone, but when considering modes of inheritance, rbcL+ITS and matK+ITS were the best barcodes for identifying Oberonia species. Furthermore, the present barcoding system has many new insights in the current Oberonia taxonomy, such as correcting species identification, resolving taxonomic uncertainties, and the underlying presence of new or cryptic species in a genus with a complex speciation history. |
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| id | doaj.art-41624aa6d5b74336b7bac09c320136d5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T21:14:59Z |
| publishDate | 2016-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-41624aa6d5b74336b7bac09c320136d52022-12-21T17:30:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-12-01710.3389/fpls.2016.01791232134DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in ChinaYuling Li0Yuling Li1Yi Tong2Yi Tong3Fuwu Xing4South China Botanical Garden, Chinese Academy of SciencesUniversity of the Chinese Academy of SciencesShanghai Chenshan Plant Science Research Center, Chinese Academy of SciencesShanghai Chenshan Botanical GardenSouth China Botanical Garden, Chinese Academy of SciencesThe orchid genus Oberonia Lindl., is a taxonomically complex genus characterized by recent species radiations and many closely related species. All Oberonia species are under conservation as listed in the CITES and the IUCN Red List Categories and Criteria. Given its difficulties in taxonomy and conservation status, Oberonia is an excellent model for developing DNA barcodes. Three analytical methods and five DNA barcoding regions (rbcL, matK, trnH-psbA, ITS and ITS2) were evaluated on 127 individuals representing 40 species and 1 variety of Oberonia from China. All the three plastid candidates tested (rbcL, matK and trnH-psbA) have a lower discriminatory power than the nuclear regions (ITS and ITS2), and ITS had the highest resolution rate (82.14%). Two to four combinations of these gene sets were not better than the ITS alone, but when considering modes of inheritance, rbcL+ITS and matK+ITS were the best barcodes for identifying Oberonia species. Furthermore, the present barcoding system has many new insights in the current Oberonia taxonomy, such as correcting species identification, resolving taxonomic uncertainties, and the underlying presence of new or cryptic species in a genus with a complex speciation history.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01791/fullRadiationITSDNA barcodingTaxonomySpecies identificationOberonia |
| spellingShingle | Yuling Li Yuling Li Yi Tong Yi Tong Fuwu Xing DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China Frontiers in Plant Science Radiation ITS DNA barcoding Taxonomy Species identification Oberonia |
| title | DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China |
| title_full | DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China |
| title_fullStr | DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China |
| title_full_unstemmed | DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China |
| title_short | DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China |
| title_sort | dna barcoding evaluation and its taxonomic implications in the recently evolved genus oberonia lindl orchidaceae in china |
| topic | Radiation ITS DNA barcoding Taxonomy Species identification Oberonia |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01791/full |
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