Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules
In this study Ginkgo biloba leaves (GBL) decoction and commercial capsules were digested using an in vitro model. Thirty-six active compounds were identified and quantified by HPLC-ESI-MS analysis based on the MS/MS patterns (precursor ions and product ions) and retention times, in comparison with r...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-12-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0033 |
| _version_ | 1826817909171683328 |
|---|---|
| author | Zhou Yubo Yang Yingxin Ma Minyan Xie Lingyun Yan Aijuan Cao Wen |
| author_facet | Zhou Yubo Yang Yingxin Ma Minyan Xie Lingyun Yan Aijuan Cao Wen |
| author_sort | Zhou Yubo |
| collection | DOAJ |
| description | In this study Ginkgo biloba leaves (GBL) decoction and commercial capsules were digested using an in vitro model. Thirty-six active compounds were identified and quantified by HPLC-ESI-MS analysis based on the MS/MS patterns (precursor ions and product ions) and retention times, in comparison with reference standards. Most compounds in GBL showed a significant decrease during intestinal digestion, with an exception of vanillic acid and biflavonoids. Bioaccessibility values of chemical compositions varied between decoction and capsules samples. Also, significant reductions of total flavonoids and total phenolic content was observed after in vitro digestion. Both, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid (ABTS) scavenging capacity decreased after gastric digestion, but increased during intestinal digestion. Nevertheless, different behaviour was observed in reducing antioxidant power (FRAP) assay. Compared to the pH of digestion, the influence of digestive enzymes on the chemical composition and antioxidant activity of GBL was relatively minor. Overall, these results may help provide a valid foundation for further investigations on bioactive compounds and the pharmacodynamics of GBL. |
| first_indexed | 2024-04-09T14:08:35Z |
| format | Article |
| id | doaj.art-416857e2356b4a9b9799d0a788a4152b |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2025-02-16T05:43:32Z |
| publishDate | 2022-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-416857e2356b4a9b9799d0a788a4152b2025-02-03T10:46:33ZengSciendoActa Pharmaceutica1846-95582022-12-0172448350710.2478/acph-2022-0033Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsulesZhou Yubo0Yang Yingxin1Ma Minyan2Xie Lingyun3Yan Aijuan4Cao Wen5College of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaCollege of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaCollege of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaCollege of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaCollege of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaCollege of Pharmacy, Zhejiang, Pharmaceutical College, Ningbo, Zhejiang, 315100, ChinaIn this study Ginkgo biloba leaves (GBL) decoction and commercial capsules were digested using an in vitro model. Thirty-six active compounds were identified and quantified by HPLC-ESI-MS analysis based on the MS/MS patterns (precursor ions and product ions) and retention times, in comparison with reference standards. Most compounds in GBL showed a significant decrease during intestinal digestion, with an exception of vanillic acid and biflavonoids. Bioaccessibility values of chemical compositions varied between decoction and capsules samples. Also, significant reductions of total flavonoids and total phenolic content was observed after in vitro digestion. Both, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid (ABTS) scavenging capacity decreased after gastric digestion, but increased during intestinal digestion. Nevertheless, different behaviour was observed in reducing antioxidant power (FRAP) assay. Compared to the pH of digestion, the influence of digestive enzymes on the chemical composition and antioxidant activity of GBL was relatively minor. Overall, these results may help provide a valid foundation for further investigations on bioactive compounds and the pharmacodynamics of GBL.https://doi.org/10.2478/acph-2022-0033ginkgo biloba l.in vitro digestionhplc-ms/mschemical compositionantioxidant propertybioaccessibility |
| spellingShingle | Zhou Yubo Yang Yingxin Ma Minyan Xie Lingyun Yan Aijuan Cao Wen Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules Acta Pharmaceutica ginkgo biloba l. in vitro digestion hplc-ms/ms chemical composition antioxidant property bioaccessibility |
| title | Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules |
| title_full | Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules |
| title_fullStr | Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules |
| title_full_unstemmed | Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules |
| title_short | Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules |
| title_sort | effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of ginkgo biloba leaves decoction and commercial capsules |
| topic | ginkgo biloba l. in vitro digestion hplc-ms/ms chemical composition antioxidant property bioaccessibility |
| url | https://doi.org/10.2478/acph-2022-0033 |
| work_keys_str_mv | AT zhouyubo effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules AT yangyingxin effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules AT maminyan effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules AT xielingyun effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules AT yanaijuan effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules AT caowen effectofinvitrogastrointestinaldigestiononthechemicalcompositionandantioxidantpropertiesofginkgobilobaleavesdecoctionandcommercialcapsules |