Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats
Background and Objectives: Brucella, causative of brucellosis, has some potential virulence factors involved in Brucella replication and its strategies to circumvent the immune response. One of them is the virB gene that encodes the type ІV secretion system proteins (T4SS) involved in intracellular...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2013-12-01
|
| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/490 |
| _version_ | 1818407888725475328 |
|---|---|
| author | Abdollah Derakhshandeh Roya Firouzi Ali Goudarztalejerd |
| author_facet | Abdollah Derakhshandeh Roya Firouzi Ali Goudarztalejerd |
| author_sort | Abdollah Derakhshandeh |
| collection | DOAJ |
| description | Background and Objectives: Brucella, causative of brucellosis, has some potential virulence factors involved in Brucella replication and its strategies to circumvent the immune response. One of them is the virB gene that encodes the type ІV secretion system proteins (T4SS) involved in intracellular replication of organism. Brucella virulence factor A (bvfA), and urease (ure) has also been described as being implicated in survival, and virulence in the hosts. The aim of this study was to investigate the B. melitensis virulence factor genes among Brucella isolated from aborted fetuses of sheep and goats in Fars province, southern Iran.
Materials and Methods: A total of 42 isolates of B. melitensis isolated from aborted fetuses between 2005-2011 in Fars province of Iran was used in this study. PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers.
Results and Conclusions: The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively. The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease. |
| first_indexed | 2024-12-14T09:35:00Z |
| format | Article |
| id | doaj.art-4168b81ec1e14adab6079c88a8d24dc0 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-14T09:35:00Z |
| publishDate | 2013-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-4168b81ec1e14adab6079c88a8d24dc02022-12-21T23:07:58ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472013-12-0154Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goatsAbdollah Derakhshandeh0Roya Firouzi1Ali Goudarztalejerd2Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.Background and Objectives: Brucella, causative of brucellosis, has some potential virulence factors involved in Brucella replication and its strategies to circumvent the immune response. One of them is the virB gene that encodes the type ІV secretion system proteins (T4SS) involved in intracellular replication of organism. Brucella virulence factor A (bvfA), and urease (ure) has also been described as being implicated in survival, and virulence in the hosts. The aim of this study was to investigate the B. melitensis virulence factor genes among Brucella isolated from aborted fetuses of sheep and goats in Fars province, southern Iran. Materials and Methods: A total of 42 isolates of B. melitensis isolated from aborted fetuses between 2005-2011 in Fars province of Iran was used in this study. PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers. Results and Conclusions: The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively. The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease.https://ijm.tums.ac.ir/index.php/ijm/article/view/490Brucella melitensisFars provinceGoatIranSheepVirulence genes (bvfA |
| spellingShingle | Abdollah Derakhshandeh Roya Firouzi Ali Goudarztalejerd Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats Iranian Journal of Microbiology Brucella melitensis Fars province Goat Iran Sheep Virulence genes (bvfA |
| title | Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats |
| title_full | Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats |
| title_fullStr | Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats |
| title_full_unstemmed | Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats |
| title_short | Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats |
| title_sort | detection of virulence genes bvfa virb and ure in brucella melitensis isolated from aborted fetuses of sheep and goats |
| topic | Brucella melitensis Fars province Goat Iran Sheep Virulence genes (bvfA |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/490 |
| work_keys_str_mv | AT abdollahderakhshandeh detectionofvirulencegenesbvfavirbandureinbrucellamelitensisisolatedfromabortedfetusesofsheepandgoats AT royafirouzi detectionofvirulencegenesbvfavirbandureinbrucellamelitensisisolatedfromabortedfetusesofsheepandgoats AT aligoudarztalejerd detectionofvirulencegenesbvfavirbandureinbrucellamelitensisisolatedfromabortedfetusesofsheepandgoats |