Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin
Abstract Because the human eye cannot visually detect the results of direct bilirubin test papers accurately and quantitatively, this study proposes four different highly collimated mini light-emitting diodes (HC mini-LEDs) as light sources for detection. First, different concentrations of bilirubin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-01-01
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| Series: | Discover Nano |
| Online Access: | https://doi.org/10.1186/s11671-024-03957-2 |
| _version_ | 1827377179075280896 |
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| author | Zhi Ting Ye Shen Fu Tseng Shang Xuan Tsou Chun Wei Tsai |
| author_facet | Zhi Ting Ye Shen Fu Tseng Shang Xuan Tsou Chun Wei Tsai |
| author_sort | Zhi Ting Ye |
| collection | DOAJ |
| description | Abstract Because the human eye cannot visually detect the results of direct bilirubin test papers accurately and quantitatively, this study proposes four different highly collimated mini light-emitting diodes (HC mini-LEDs) as light sources for detection. First, different concentrations of bilirubin were oxidized to biliverdin by FeCl3 on the test paper, and pictures were obtained with a smartphone. Next, the red, green, and blue (RGB) channels of the pictures were separated to average grayscale values, and their linear relationship with the direct bilirubin concentration was analyzed to detect bilirubin on the test paper noninvasively and quantitatively. The experimental results showed that when green HC mini-LEDs were used as the light sources and image analysis was performed using the G channel, for a direct bilirubin concentration range of 0.1–2 mg/dL, the G channel determination coefficient (R2) reached 0.9523 and limit of detection was 0.459 mg/dL. The detection method proposed herein has advantages such as rapid analysis, noninvasive detection, and digitization according to RGB grayscale changes in the images of the detection test paper. |
| first_indexed | 2024-03-08T12:35:15Z |
| format | Article |
| id | doaj.art-416dfffa9566477e80954a2824aab948 |
| institution | Directory Open Access Journal |
| issn | 2731-9229 |
| language | English |
| last_indexed | 2024-03-08T12:35:15Z |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Nano |
| spelling | doaj.art-416dfffa9566477e80954a2824aab9482024-01-21T12:31:16ZengSpringerDiscover Nano2731-92292024-01-0119111010.1186/s11671-024-03957-2Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubinZhi Ting Ye0Shen Fu Tseng1Shang Xuan Tsou2Chun Wei Tsai3Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng UniversityDepartment of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng UniversityDepartment of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng UniversityGraduate Institute of Photonics and Optoelectronics, National Taiwan UniversityAbstract Because the human eye cannot visually detect the results of direct bilirubin test papers accurately and quantitatively, this study proposes four different highly collimated mini light-emitting diodes (HC mini-LEDs) as light sources for detection. First, different concentrations of bilirubin were oxidized to biliverdin by FeCl3 on the test paper, and pictures were obtained with a smartphone. Next, the red, green, and blue (RGB) channels of the pictures were separated to average grayscale values, and their linear relationship with the direct bilirubin concentration was analyzed to detect bilirubin on the test paper noninvasively and quantitatively. The experimental results showed that when green HC mini-LEDs were used as the light sources and image analysis was performed using the G channel, for a direct bilirubin concentration range of 0.1–2 mg/dL, the G channel determination coefficient (R2) reached 0.9523 and limit of detection was 0.459 mg/dL. The detection method proposed herein has advantages such as rapid analysis, noninvasive detection, and digitization according to RGB grayscale changes in the images of the detection test paper.https://doi.org/10.1186/s11671-024-03957-2 |
| spellingShingle | Zhi Ting Ye Shen Fu Tseng Shang Xuan Tsou Chun Wei Tsai Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin Discover Nano |
| title | Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin |
| title_full | Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin |
| title_fullStr | Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin |
| title_full_unstemmed | Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin |
| title_short | Spectral analysis with highly collimated mini-LEDs as light sources for quantitative detection of direct bilirubin |
| title_sort | spectral analysis with highly collimated mini leds as light sources for quantitative detection of direct bilirubin |
| url | https://doi.org/10.1186/s11671-024-03957-2 |
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