Chryseobacterium indologenes, novel mannanase-producing bacteria

Mannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two...

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Main Authors: Surachai Rattanasuk, Mareina Ketudat-Cairns
Format: Article
Language:English
Published: Prince of Songkla University 2009-10-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdf
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author Surachai Rattanasuk
Mareina Ketudat-Cairns
author_facet Surachai Rattanasuk
Mareina Ketudat-Cairns
author_sort Surachai Rattanasuk
collection DOAJ
description Mannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two genera of bacteria were isolated from Thai soil samples,fermented coconut, and fertilizer. Screening was carried out on agar plates containing mannan stained with iodine solution.The bacteria were identified by partial 16S rRNA gene sequence, biochemical test and morphology, respectively. The mannanase activity was determined by zymogram and DNS method. Two strains of bacteria with mannanase activity were identified as Bacillus and Chryseobacterium. This is the first report of mannanase-producing Chryseobacterium.
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spelling doaj.art-416ea591a58e47968aef2d51381dc1572022-12-22T03:33:10ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952009-10-01314395399Chryseobacterium indologenes, novel mannanase-producing bacteriaSurachai RattanasukMareina Ketudat-CairnsMannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two genera of bacteria were isolated from Thai soil samples,fermented coconut, and fertilizer. Screening was carried out on agar plates containing mannan stained with iodine solution.The bacteria were identified by partial 16S rRNA gene sequence, biochemical test and morphology, respectively. The mannanase activity was determined by zymogram and DNS method. Two strains of bacteria with mannanase activity were identified as Bacillus and Chryseobacterium. This is the first report of mannanase-producing Chryseobacterium.http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdfChryseobacteriumBacilluszymogrammannanase-producing bacteria
spellingShingle Surachai Rattanasuk
Mareina Ketudat-Cairns
Chryseobacterium indologenes, novel mannanase-producing bacteria
Songklanakarin Journal of Science and Technology (SJST)
Chryseobacterium
Bacillus
zymogram
mannanase-producing bacteria
title Chryseobacterium indologenes, novel mannanase-producing bacteria
title_full Chryseobacterium indologenes, novel mannanase-producing bacteria
title_fullStr Chryseobacterium indologenes, novel mannanase-producing bacteria
title_full_unstemmed Chryseobacterium indologenes, novel mannanase-producing bacteria
title_short Chryseobacterium indologenes, novel mannanase-producing bacteria
title_sort chryseobacterium indologenes novel mannanase producing bacteria
topic Chryseobacterium
Bacillus
zymogram
mannanase-producing bacteria
url http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdf
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