Chryseobacterium indologenes, novel mannanase-producing bacteria
Mannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two...
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Format: | Article |
Language: | English |
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Prince of Songkla University
2009-10-01
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Series: | Songklanakarin Journal of Science and Technology (SJST) |
Subjects: | |
Online Access: | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdf |
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author | Surachai Rattanasuk Mareina Ketudat-Cairns |
author_facet | Surachai Rattanasuk Mareina Ketudat-Cairns |
author_sort | Surachai Rattanasuk |
collection | DOAJ |
description | Mannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two genera of bacteria were isolated from Thai soil samples,fermented coconut, and fertilizer. Screening was carried out on agar plates containing mannan stained with iodine solution.The bacteria were identified by partial 16S rRNA gene sequence, biochemical test and morphology, respectively. The mannanase activity was determined by zymogram and DNS method. Two strains of bacteria with mannanase activity were identified as Bacillus and Chryseobacterium. This is the first report of mannanase-producing Chryseobacterium. |
first_indexed | 2024-04-12T12:25:25Z |
format | Article |
id | doaj.art-416ea591a58e47968aef2d51381dc157 |
institution | Directory Open Access Journal |
issn | 0125-3395 |
language | English |
last_indexed | 2024-04-12T12:25:25Z |
publishDate | 2009-10-01 |
publisher | Prince of Songkla University |
record_format | Article |
series | Songklanakarin Journal of Science and Technology (SJST) |
spelling | doaj.art-416ea591a58e47968aef2d51381dc1572022-12-22T03:33:10ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952009-10-01314395399Chryseobacterium indologenes, novel mannanase-producing bacteriaSurachai RattanasukMareina Ketudat-CairnsMannanase is a mannan degrading enzyme which is produced by microorganisms, including bacteria. This enzyme can be used in many industrial processes as well as for improving the quality of animal feeds. The aim of the present study was toscreen and characterize the mannanase-producing bacteria. Two genera of bacteria were isolated from Thai soil samples,fermented coconut, and fertilizer. Screening was carried out on agar plates containing mannan stained with iodine solution.The bacteria were identified by partial 16S rRNA gene sequence, biochemical test and morphology, respectively. The mannanase activity was determined by zymogram and DNS method. Two strains of bacteria with mannanase activity were identified as Bacillus and Chryseobacterium. This is the first report of mannanase-producing Chryseobacterium.http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdfChryseobacteriumBacilluszymogrammannanase-producing bacteria |
spellingShingle | Surachai Rattanasuk Mareina Ketudat-Cairns Chryseobacterium indologenes, novel mannanase-producing bacteria Songklanakarin Journal of Science and Technology (SJST) Chryseobacterium Bacillus zymogram mannanase-producing bacteria |
title | Chryseobacterium indologenes, novel mannanase-producing bacteria |
title_full | Chryseobacterium indologenes, novel mannanase-producing bacteria |
title_fullStr | Chryseobacterium indologenes, novel mannanase-producing bacteria |
title_full_unstemmed | Chryseobacterium indologenes, novel mannanase-producing bacteria |
title_short | Chryseobacterium indologenes, novel mannanase-producing bacteria |
title_sort | chryseobacterium indologenes novel mannanase producing bacteria |
topic | Chryseobacterium Bacillus zymogram mannanase-producing bacteria |
url | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-4/0125-3395-31-4-395-399.pdf |
work_keys_str_mv | AT surachairattanasuk chryseobacteriumindologenesnovelmannanaseproducingbacteria AT mareinaketudatcairns chryseobacteriumindologenesnovelmannanaseproducingbacteria |