Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock
This study employs biomass growth analyses and 13C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Metabolic Engineering Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030120300080 |
| _version_ | 1818458376040873984 |
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| author | Alyssa M. Worland Jeffrey J. Czajka Yun Xing Willie F. Harper, Jr. Aryiana Moore Zhengyang Xiao Zhenlin Han Yechun Wang Wei Wen Su Yinjie J. Tang |
| author_facet | Alyssa M. Worland Jeffrey J. Czajka Yun Xing Willie F. Harper, Jr. Aryiana Moore Zhengyang Xiao Zhenlin Han Yechun Wang Wei Wen Su Yinjie J. Tang |
| author_sort | Alyssa M. Worland |
| collection | DOAJ |
| description | This study employs biomass growth analyses and 13C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock. |
| first_indexed | 2024-12-14T22:57:28Z |
| format | Article |
| id | doaj.art-416fd5276e0d498f91a46c45f8ebcecd |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-12-14T22:57:28Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-416fd5276e0d498f91a46c45f8ebcecd2022-12-21T22:44:33ZengElsevierMetabolic Engineering Communications2214-03012020-12-0111e00130Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstockAlyssa M. Worland0Jeffrey J. Czajka1Yun Xing2Willie F. Harper, Jr.3Aryiana Moore4Zhengyang Xiao5Zhenlin Han6Yechun Wang7Wei Wen Su8Yinjie J. Tang9Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO, 63130, USADepartment of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO, 63130, USADepartment of Systems Engineering and Management, Air Force Institute of Technology, Wright-Patterson AFB, OH, 45433, USADepartment of Systems Engineering and Management, Air Force Institute of Technology, Wright-Patterson AFB, OH, 45433, USADepartment of Environmental Engineering, Johns Hopkins University, Baltimore, MD, 21218, USADepartment of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO, 63130, USADepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USAArch Innotek, LLC, 400 Farmington Ave, Farmington, CT, 06032, USADepartment of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USADepartment of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO, 63130, USA; Corresponding author.This study employs biomass growth analyses and 13C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast's biomass yields and cell sizes, but decreases its protein content (<20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock.http://www.sciencedirect.com/science/article/pii/S221403012030008013C-isotope tracingβ-caroteneCatabolite repressionDimorphismMetabolic network |
| spellingShingle | Alyssa M. Worland Jeffrey J. Czajka Yun Xing Willie F. Harper, Jr. Aryiana Moore Zhengyang Xiao Zhenlin Han Yechun Wang Wei Wen Su Yinjie J. Tang Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock Metabolic Engineering Communications 13C-isotope tracing β-carotene Catabolite repression Dimorphism Metabolic network |
| title | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
| title_full | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
| title_fullStr | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
| title_full_unstemmed | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
| title_short | Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock |
| title_sort | analysis of yarrowia lipolytica growth catabolism and terpenoid biosynthesis during utilization of lipid derived feedstock |
| topic | 13C-isotope tracing β-carotene Catabolite repression Dimorphism Metabolic network |
| url | http://www.sciencedirect.com/science/article/pii/S2214030120300080 |
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