Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2
Summary: Skeletal muscle is a highly plastic organ that adapts to different metabolic states or functional demands. This study explored the impact of permanent glucose restriction (GR) on skeletal muscle composition and metabolism. Using Glut4m mice with defective glucose transporter 4, we conducted...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223026676 |
| _version_ | 1797398555557625856 |
|---|---|
| author | Kaiyi Zhang Ning Xie Huaqiong Ye Jiakun Miao Boce Xia Yu Yang Huanqi Peng Shuang Xu Tianwen Wu Cong Tao Jinxue Ruan Yanfang Wang Shulin Yang |
| author_facet | Kaiyi Zhang Ning Xie Huaqiong Ye Jiakun Miao Boce Xia Yu Yang Huanqi Peng Shuang Xu Tianwen Wu Cong Tao Jinxue Ruan Yanfang Wang Shulin Yang |
| author_sort | Kaiyi Zhang |
| collection | DOAJ |
| description | Summary: Skeletal muscle is a highly plastic organ that adapts to different metabolic states or functional demands. This study explored the impact of permanent glucose restriction (GR) on skeletal muscle composition and metabolism. Using Glut4m mice with defective glucose transporter 4, we conducted multi-omics analyses at different ages and after low-intensity treadmill training. The oxidative fibers were significantly increased in Glut4m muscles. Mechanistically, GR activated AMPK pathway, promoting mitochondrial function and beneficial myokine expression, and facilitated slow fiber formation via CaMK2 pathway. Phosphorylation-activated Perm1 may synergize AMPK and CaMK2 signaling. Besides, MAPK and CDK kinases were also implicated in skeletal muscle protein phosphorylation during GR response. This study provides a comprehensive signaling network demonstrating how GR influences muscle fiber types and metabolic patterns. These insights offer valuable data for understanding oxidative fiber formation mechanisms and identifying clinical targets for metabolic diseases. |
| first_indexed | 2024-03-09T01:27:11Z |
| format | Article |
| id | doaj.art-41701b2e26f24b90b443040f37adc36b |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-09T01:27:11Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-41701b2e26f24b90b443040f37adc36b2023-12-10T06:16:59ZengElsevieriScience2589-00422024-01-01271108590Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2Kaiyi Zhang0Ning Xie1Huaqiong Ye2Jiakun Miao3Boce Xia4Yu Yang5Huanqi Peng6Shuang Xu7Tianwen Wu8Cong Tao9Jinxue Ruan10Yanfang Wang11Shulin Yang12State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China; Precision Livestock and Nutrition Unit, Gembloux Agro-Bio Tech, TERRA Teaching and Research Centre, Liège University, 5030 Gembloux, BelgiumState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China; Corresponding authorState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China; Corresponding authorState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China; Corresponding authorSummary: Skeletal muscle is a highly plastic organ that adapts to different metabolic states or functional demands. This study explored the impact of permanent glucose restriction (GR) on skeletal muscle composition and metabolism. Using Glut4m mice with defective glucose transporter 4, we conducted multi-omics analyses at different ages and after low-intensity treadmill training. The oxidative fibers were significantly increased in Glut4m muscles. Mechanistically, GR activated AMPK pathway, promoting mitochondrial function and beneficial myokine expression, and facilitated slow fiber formation via CaMK2 pathway. Phosphorylation-activated Perm1 may synergize AMPK and CaMK2 signaling. Besides, MAPK and CDK kinases were also implicated in skeletal muscle protein phosphorylation during GR response. This study provides a comprehensive signaling network demonstrating how GR influences muscle fiber types and metabolic patterns. These insights offer valuable data for understanding oxidative fiber formation mechanisms and identifying clinical targets for metabolic diseases.http://www.sciencedirect.com/science/article/pii/S2589004223026676CompMetabolomicsOmicsGenomicsProteomics |
| spellingShingle | Kaiyi Zhang Ning Xie Huaqiong Ye Jiakun Miao Boce Xia Yu Yang Huanqi Peng Shuang Xu Tianwen Wu Cong Tao Jinxue Ruan Yanfang Wang Shulin Yang Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 iScience Comp Metabolomics Omics Genomics Proteomics |
| title | Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 |
| title_full | Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 |
| title_fullStr | Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 |
| title_full_unstemmed | Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 |
| title_short | Glucose restriction enhances oxidative fiber formation: A multi-omic signal network involving AMPK and CaMK2 |
| title_sort | glucose restriction enhances oxidative fiber formation a multi omic signal network involving ampk and camk2 |
| topic | Comp Metabolomics Omics Genomics Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223026676 |
| work_keys_str_mv | AT kaiyizhang glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT ningxie glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT huaqiongye glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT jiakunmiao glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT bocexia glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT yuyang glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT huanqipeng glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT shuangxu glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT tianwenwu glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT congtao glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT jinxueruan glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT yanfangwang glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 AT shulinyang glucoserestrictionenhancesoxidativefiberformationamultiomicsignalnetworkinvolvingampkandcamk2 |