LC-MS/MS method for the detection of multiple classes of shellfish toxins
Marine shellfish toxins are seafood safety problems of global concern. Herein the analysis of six shellfish toxins, regulated by European Union, with one single run by LC-MS/MS with acidic mobile phase was developed. After 80% methanol extraction of the shellfish toxins, the crude extract was subjec...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2019-06-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201903-0004_lc-ms-ms-method-for-the-detection-of-multiple-classes-of-shellfish-toxins.php |
| _version_ | 1797899600490659840 |
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| author | Ling Jan Chiou Tai Sheng Yeh Jiing Chuan Chen |
| author_facet | Ling Jan Chiou Tai Sheng Yeh Jiing Chuan Chen |
| author_sort | Ling Jan Chiou |
| collection | DOAJ |
| description | Marine shellfish toxins are seafood safety problems of global concern. Herein the analysis of six shellfish toxins, regulated by European Union, with one single run by LC-MS/MS with acidic mobile phase was developed. After 80% methanol extraction of the shellfish toxins, the crude extract was subjected to HLB SPE cleanup before LC-MS/MS analysis. The method was validated according to Commission Decision 2002/657/EC. For azaspiracid-1 (AZA1), domoic acid (DA), dinophysistoxin-1 (DTX1), okadaic acid (OA), pectenotoxin-2 (PTX2), and yessotoxin (YTX) toxins the recovery rate was 99.4, 92.7, 114.1, 90.2, 115.2 and 87.8%, respectively. The intra-day relative standard deviation (RSD) was less than 5% for all of the shellfish toxins except DA. The inter-day RSD was less than 5% for AZA1, DTX1, PTX2, YTX, 7.85% for DA, and 14.63% for OA. The decision limit (CCα) and detection capability (CCβ) for AZA1 were 13.6 and 14.8 ppb; for DA they were 1883 and 2051 ppb; DTX1 12.3 and 13.4 ppb; OA 8.0 and 8.7 ppb; PTX2 12.1 and 13.2 ppb; YTX 36.9 and 40.1 ppb. |
| first_indexed | 2024-04-10T08:32:33Z |
| format | Article |
| id | doaj.art-4176d0b0e2f345218afd2c9fb5885877 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:32:33Z |
| publishDate | 2019-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-4176d0b0e2f345218afd2c9fb58858772023-02-23T03:28:36ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172019-06-0137317317910.17221/125/2018-CJFScjf-201903-0004LC-MS/MS method for the detection of multiple classes of shellfish toxinsLing Jan Chiou0Tai Sheng Yeh1Jiing Chuan Chen2Department of Health Business Administration, Meiho University, Pingtung, Taiwan, R.O.C.Department of Food Science and Nutrition, Meiho University, Pingtung, Taiwan, R.O.C.Department of Food Science and Nutrition, Meiho University, Pingtung, Taiwan, R.O.C.Marine shellfish toxins are seafood safety problems of global concern. Herein the analysis of six shellfish toxins, regulated by European Union, with one single run by LC-MS/MS with acidic mobile phase was developed. After 80% methanol extraction of the shellfish toxins, the crude extract was subjected to HLB SPE cleanup before LC-MS/MS analysis. The method was validated according to Commission Decision 2002/657/EC. For azaspiracid-1 (AZA1), domoic acid (DA), dinophysistoxin-1 (DTX1), okadaic acid (OA), pectenotoxin-2 (PTX2), and yessotoxin (YTX) toxins the recovery rate was 99.4, 92.7, 114.1, 90.2, 115.2 and 87.8%, respectively. The intra-day relative standard deviation (RSD) was less than 5% for all of the shellfish toxins except DA. The inter-day RSD was less than 5% for AZA1, DTX1, PTX2, YTX, 7.85% for DA, and 14.63% for OA. The decision limit (CCα) and detection capability (CCβ) for AZA1 were 13.6 and 14.8 ppb; for DA they were 1883 and 2051 ppb; DTX1 12.3 and 13.4 ppb; OA 8.0 and 8.7 ppb; PTX2 12.1 and 13.2 ppb; YTX 36.9 and 40.1 ppb.https://cjfs.agriculturejournals.cz/artkey/cjf-201903-0004_lc-ms-ms-method-for-the-detection-of-multiple-classes-of-shellfish-toxins.phpacidic mobile phasediarrheic shellfish toxinlc-ms/ms |
| spellingShingle | Ling Jan Chiou Tai Sheng Yeh Jiing Chuan Chen LC-MS/MS method for the detection of multiple classes of shellfish toxins Czech Journal of Food Sciences acidic mobile phase diarrheic shellfish toxin lc-ms/ms |
| title | LC-MS/MS method for the detection of multiple classes of shellfish toxins |
| title_full | LC-MS/MS method for the detection of multiple classes of shellfish toxins |
| title_fullStr | LC-MS/MS method for the detection of multiple classes of shellfish toxins |
| title_full_unstemmed | LC-MS/MS method for the detection of multiple classes of shellfish toxins |
| title_short | LC-MS/MS method for the detection of multiple classes of shellfish toxins |
| title_sort | lc ms ms method for the detection of multiple classes of shellfish toxins |
| topic | acidic mobile phase diarrheic shellfish toxin lc-ms/ms |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201903-0004_lc-ms-ms-method-for-the-detection-of-multiple-classes-of-shellfish-toxins.php |
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