Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection
Abstract Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunog...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-46714-w |
| _version_ | 1797247121643012096 |
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| author | Carlos Ávila-Nieto Júlia Vergara-Alert Pep Amengual-Rigo Erola Ainsua-Enrich Marco Brustolin María Luisa Rodríguez de la Concepción Núria Pedreño-Lopez Jordi Rodon Victor Urrea Edwards Pradenas Silvia Marfil Ester Ballana Eva Riveira-Muñoz Mònica Pérez Núria Roca Ferran Tarrés-Freixas Guillermo Cantero Anna Pons-Grífols Carla Rovirosa Carmen Aguilar-Gurrieri Raquel Ortiz Ana Barajas Benjamin Trinité Rosalba Lepore Jordana Muñoz-Basagoiti Daniel Perez-Zsolt Nuria Izquierdo-Useros Alfonso Valencia Julià Blanco Victor Guallar Bonaventura Clotet Joaquim Segalés Jorge Carrillo |
| author_facet | Carlos Ávila-Nieto Júlia Vergara-Alert Pep Amengual-Rigo Erola Ainsua-Enrich Marco Brustolin María Luisa Rodríguez de la Concepción Núria Pedreño-Lopez Jordi Rodon Victor Urrea Edwards Pradenas Silvia Marfil Ester Ballana Eva Riveira-Muñoz Mònica Pérez Núria Roca Ferran Tarrés-Freixas Guillermo Cantero Anna Pons-Grífols Carla Rovirosa Carmen Aguilar-Gurrieri Raquel Ortiz Ana Barajas Benjamin Trinité Rosalba Lepore Jordana Muñoz-Basagoiti Daniel Perez-Zsolt Nuria Izquierdo-Useros Alfonso Valencia Julià Blanco Victor Guallar Bonaventura Clotet Joaquim Segalés Jorge Carrillo |
| author_sort | Carlos Ávila-Nieto |
| collection | DOAJ |
| description | Abstract Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunogenicity. However, S-2P is still produced at low levels. Here, we describe the V987H mutation that increases by two-fold the production of the recombinant Spike and the exposure of the receptor binding domain (RBD). S-V987H immunogenicity is similar to S-2P in mice and golden Syrian hamsters (GSH), and superior to a monomeric RBD. S-V987H immunization confer full protection against severe disease in K18-hACE2 mice and GSH upon SARS-CoV-2 challenge (D614G or B.1.351 variants). Furthermore, S-V987H immunized K18-hACE2 mice show a faster tissue viral clearance than RBD- or S-2P-vaccinated animals challenged with D614G, B.1.351 or Omicron BQ1.1 variants. Thus, S-V987H protein might be considered for future SARS-CoV-2 vaccines development. |
| first_indexed | 2024-04-24T19:53:40Z |
| format | Article |
| id | doaj.art-417894952ca54f88947455ec8d336df7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T19:53:40Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-417894952ca54f88947455ec8d336df72024-03-24T12:26:45ZengNature PortfolioNature Communications2041-17232024-03-0115111810.1038/s41467-024-46714-wImmunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infectionCarlos Ávila-Nieto0Júlia Vergara-Alert1Pep Amengual-Rigo2Erola Ainsua-Enrich3Marco Brustolin4María Luisa Rodríguez de la Concepción5Núria Pedreño-Lopez6Jordi Rodon7Victor Urrea8Edwards Pradenas9Silvia Marfil10Ester Ballana11Eva Riveira-Muñoz12Mònica Pérez13Núria Roca14Ferran Tarrés-Freixas15Guillermo Cantero16Anna Pons-Grífols17Carla Rovirosa18Carmen Aguilar-Gurrieri19Raquel Ortiz20Ana Barajas21Benjamin Trinité22Rosalba Lepore23Jordana Muñoz-Basagoiti24Daniel Perez-Zsolt25Nuria Izquierdo-Useros26Alfonso Valencia27Julià Blanco28Victor Guallar29Bonaventura Clotet30Joaquim Segalés31Jorge Carrillo32IrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)Life Sciences Department, Barcelona Supercomputing Center (BSC)IrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)IrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)IrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)Unitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)IrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)IrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiLife Sciences Department, Barcelona Supercomputing Center (BSC)IrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiIrsiCaixa AIDS Research Institute, Campus Can RutiLife Sciences Department, Barcelona Supercomputing Center (BSC)IrsiCaixa AIDS Research Institute, Campus Can RutiLife Sciences Department, Barcelona Supercomputing Center (BSC)IrsiCaixa AIDS Research Institute, Campus Can RutiUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB)IrsiCaixa AIDS Research Institute, Campus Can RutiAbstract Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the coronavirus disease 2019 pandemic. Most vaccines are based on a mutated version of the Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield and immunogenicity. However, S-2P is still produced at low levels. Here, we describe the V987H mutation that increases by two-fold the production of the recombinant Spike and the exposure of the receptor binding domain (RBD). S-V987H immunogenicity is similar to S-2P in mice and golden Syrian hamsters (GSH), and superior to a monomeric RBD. S-V987H immunization confer full protection against severe disease in K18-hACE2 mice and GSH upon SARS-CoV-2 challenge (D614G or B.1.351 variants). Furthermore, S-V987H immunized K18-hACE2 mice show a faster tissue viral clearance than RBD- or S-2P-vaccinated animals challenged with D614G, B.1.351 or Omicron BQ1.1 variants. Thus, S-V987H protein might be considered for future SARS-CoV-2 vaccines development.https://doi.org/10.1038/s41467-024-46714-w |
| spellingShingle | Carlos Ávila-Nieto Júlia Vergara-Alert Pep Amengual-Rigo Erola Ainsua-Enrich Marco Brustolin María Luisa Rodríguez de la Concepción Núria Pedreño-Lopez Jordi Rodon Victor Urrea Edwards Pradenas Silvia Marfil Ester Ballana Eva Riveira-Muñoz Mònica Pérez Núria Roca Ferran Tarrés-Freixas Guillermo Cantero Anna Pons-Grífols Carla Rovirosa Carmen Aguilar-Gurrieri Raquel Ortiz Ana Barajas Benjamin Trinité Rosalba Lepore Jordana Muñoz-Basagoiti Daniel Perez-Zsolt Nuria Izquierdo-Useros Alfonso Valencia Julià Blanco Victor Guallar Bonaventura Clotet Joaquim Segalés Jorge Carrillo Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection Nature Communications |
| title | Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection |
| title_full | Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection |
| title_fullStr | Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection |
| title_full_unstemmed | Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection |
| title_short | Immunization with V987H-stabilized Spike glycoprotein protects K18-hACE2 mice and golden Syrian hamsters upon SARS-CoV-2 infection |
| title_sort | immunization with v987h stabilized spike glycoprotein protects k18 hace2 mice and golden syrian hamsters upon sars cov 2 infection |
| url | https://doi.org/10.1038/s41467-024-46714-w |
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