A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis
Context: A high-performance thin layer chromatography (HPTLC) is an analytical technique, which can be used for the determination of constituents or marker components in various parts of the plants. Earlier studies have estimated phytoconstituents from the stem and other aerial plant parts of Cissus...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2016-08-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol4/jppres16.118_4.4.159.pdf |
| _version_ | 1818569495364501504 |
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| author | Vandana Jain Vedang Kinjawadekar Kirti Laddha |
| author_facet | Vandana Jain Vedang Kinjawadekar Kirti Laddha |
| author_sort | Vandana Jain |
| collection | DOAJ |
| description | Context: A high-performance thin layer chromatography (HPTLC) is an analytical technique, which can be used for the determination of constituents or marker components in various parts of the plants. Earlier studies have estimated phytoconstituents from the stem and other aerial plant parts of Cissus quadrangularis Linn. Estimation of hydrocarbons can also be successfully done using HPTLC technique using suitable derivatization.
Aims: To develop and validate a simple and rapid method for the estimation of long chain aliphatic hydrocarbons from the leaves of C. quadrangularis using HPTLC technique.
Methods: Precoated silica gel 60 F254 plates were used as stationary phase. The mobile phase used was hexane (100 %). The detection of spots was carried out using berberine sulphate as detecting reagent.
Results: The method was validated in terms of linearity, sensitivity, accuracy, and precision. Linearity range was found to be 2-10 µg/mL, limit of detection 0.127 µg/mL, and limit of quantification 0.384 µg/mL.
Conclusions: A novel, simple, accurate, precise and sensitive HPTLC method has been developed and validated for the estimation of long chain aliphatic hydrocarbons obtained from the leaves of C. quadrangularis Linn. |
| first_indexed | 2024-12-14T06:48:46Z |
| format | Article |
| id | doaj.art-417c785a35cc4d52bbdf08b5773daf68 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-14T06:48:46Z |
| publishDate | 2016-08-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-417c785a35cc4d52bbdf08b5773daf682022-12-21T23:12:57ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502016-08-0144159164A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularisVandana Jain0Vedang Kinjawadekar1Kirti Laddha2Oriental College of Pharmacy, Sanpada, Navi Mumbai 400705, India.Oriental College of Pharmacy, Sanpada, Navi Mumbai 400705, India.Medicinal Natural Product Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology, Matunga (E), Mumbai 400 019, India.Context: A high-performance thin layer chromatography (HPTLC) is an analytical technique, which can be used for the determination of constituents or marker components in various parts of the plants. Earlier studies have estimated phytoconstituents from the stem and other aerial plant parts of Cissus quadrangularis Linn. Estimation of hydrocarbons can also be successfully done using HPTLC technique using suitable derivatization. Aims: To develop and validate a simple and rapid method for the estimation of long chain aliphatic hydrocarbons from the leaves of C. quadrangularis using HPTLC technique. Methods: Precoated silica gel 60 F254 plates were used as stationary phase. The mobile phase used was hexane (100 %). The detection of spots was carried out using berberine sulphate as detecting reagent. Results: The method was validated in terms of linearity, sensitivity, accuracy, and precision. Linearity range was found to be 2-10 µg/mL, limit of detection 0.127 µg/mL, and limit of quantification 0.384 µg/mL. Conclusions: A novel, simple, accurate, precise and sensitive HPTLC method has been developed and validated for the estimation of long chain aliphatic hydrocarbons obtained from the leaves of C. quadrangularis Linn.http://jppres.com/jppres/pdf/vol4/jppres16.118_4.4.159.pdfBerberine sulphatehigh-performance thin layer chromatographylong chain aliphatic hydrocarbons |
| spellingShingle | Vandana Jain Vedang Kinjawadekar Kirti Laddha A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis Journal of Pharmacy & Pharmacognosy Research Berberine sulphate high-performance thin layer chromatography long chain aliphatic hydrocarbons |
| title | A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis |
| title_full | A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis |
| title_fullStr | A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis |
| title_full_unstemmed | A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis |
| title_short | A novel high-performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from Cissus quadrangularis |
| title_sort | novel high performance thin layer chromatography method for quantification of long chain aliphatic hydrocarbons from cissus quadrangularis |
| topic | Berberine sulphate high-performance thin layer chromatography long chain aliphatic hydrocarbons |
| url | http://jppres.com/jppres/pdf/vol4/jppres16.118_4.4.159.pdf |
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