Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides.
Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3873978?pdf=render |
| _version_ | 1819111792216899584 |
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| author | Shinichi Nishimura Kumiko Ishii Kunihiko Iwamoto Yuko Arita Shigeki Matsunaga Yoshiko Ohno-Iwashita Satoshi B Sato Hideaki Kakeya Toshihide Kobayashi Minoru Yoshida |
| author_facet | Shinichi Nishimura Kumiko Ishii Kunihiko Iwamoto Yuko Arita Shigeki Matsunaga Yoshiko Ohno-Iwashita Satoshi B Sato Hideaki Kakeya Toshihide Kobayashi Minoru Yoshida |
| author_sort | Shinichi Nishimura |
| collection | DOAJ |
| description | Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions. |
| first_indexed | 2024-12-22T04:03:14Z |
| format | Article |
| id | doaj.art-417d1998049e4eb59960a9264088c916 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:03:14Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-417d1998049e4eb59960a9264088c9162022-12-21T18:39:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8371610.1371/journal.pone.0083716Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides.Shinichi NishimuraKumiko IshiiKunihiko IwamotoYuko AritaShigeki MatsunagaYoshiko Ohno-IwashitaSatoshi B SatoHideaki KakeyaToshihide KobayashiMinoru YoshidaCholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act as new sterol probes in mammalian cultured cells. The fluorescent probes recognized cholesterol molecules and bound to liposomes in a cholesterol-concentration dependent manner. The probes showed patchy distribution in the plasma membrane, while they stained specific organelle in the cytoplasm. These data suggest that fTNMs will be valuable sterol probes for studies on the role of sterols in the biological membrane under a variety of experimental conditions.http://europepmc.org/articles/PMC3873978?pdf=render |
| spellingShingle | Shinichi Nishimura Kumiko Ishii Kunihiko Iwamoto Yuko Arita Shigeki Matsunaga Yoshiko Ohno-Iwashita Satoshi B Sato Hideaki Kakeya Toshihide Kobayashi Minoru Yoshida Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. PLoS ONE |
| title | Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. |
| title_full | Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. |
| title_fullStr | Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. |
| title_full_unstemmed | Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. |
| title_short | Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides. |
| title_sort | visualization of sterol rich membrane domains with fluorescently labeled theonellamides |
| url | http://europepmc.org/articles/PMC3873978?pdf=render |
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