Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats
Our previous studies suggested that Alaska pollack protein (APP) intake increases skeletal muscle mass and that it may cause a slow-to-fast shift in muscle fiber type in rats fed a high-fat diet after 56 days of feeding. In this study, we explored whether dietary APP induces acute and sustainable sk...
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MDPI AG
2022-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/3/547 |
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| author | Kenji Uchida Mina Fujitani Takafumi Mizushige Fuminori Kawabata Kohsuke Hayamizu Keisuke Uozumi Yuma Hara Mariko Sawai Ryota Uehigashi Shinji Okada Naoko Goto-Inoue Mizuki Morisasa Taro Kishida |
| author_facet | Kenji Uchida Mina Fujitani Takafumi Mizushige Fuminori Kawabata Kohsuke Hayamizu Keisuke Uozumi Yuma Hara Mariko Sawai Ryota Uehigashi Shinji Okada Naoko Goto-Inoue Mizuki Morisasa Taro Kishida |
| author_sort | Kenji Uchida |
| collection | DOAJ |
| description | Our previous studies suggested that Alaska pollack protein (APP) intake increases skeletal muscle mass and that it may cause a slow-to-fast shift in muscle fiber type in rats fed a high-fat diet after 56 days of feeding. In this study, we explored whether dietary APP induces acute and sustainable skeletal muscle hypertrophy in rats fed a normal-fat diet. Male 5-week-old Sprague–Dawley rats were divided into four groups and fed a purified ingredient-based high-fat diet or a purified ingredient-based normal-fat diet with casein or APP, containing the same amount of crude protein. Dietary APP significantly increased gastrocnemius muscle mass (105~110%) after 2, 7 days of feeding, regardless of dietary fat content. Rats were separated into two groups and fed a normal-fat diet with casein or APP. Dietary APP significantly increased gastrocnemius muscle mass (110%) after 56 days of feeding. Dietary APP significantly increased the cross-sectional area of the gastrocnemius skeletal muscle and collagen-rich connective tissue after 7 days of feeding. It decreased the gene expression of <i>Mstn</i> /Myostatin, <i>Trim63</i>/MuRF1, and <i>Fbxo32</i>/atrogin-1, but not other gene expression, such as serum IGF-1 after 7 days of feeding. No differences were observed between casein and APP groups with respect to the percentage of Type I, Type IIA, and Type IIX or IIB fibers, as determined by myosin ATPase staining after 7 days of feeding. In the similar experiment, the puromycin-labeled peptides were not different between dietary casein and APP after 2 days of feeding. These results demonstrate that APP induces acute and sustainable skeletal muscle hypertrophy in rats, regardless of dietary fat content. Dietary APP, as a daily protein source, may be an approach for maintaining or increasing muscle mass. |
| first_indexed | 2024-03-09T23:21:17Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T23:21:17Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-417d73c301a7426290ae16a2bb39477d2023-11-23T17:25:35ZengMDPI AGNutrients2072-66432022-01-0114354710.3390/nu14030547Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in RatsKenji Uchida0Mina Fujitani1Takafumi Mizushige2Fuminori Kawabata3Kohsuke Hayamizu4Keisuke Uozumi5Yuma Hara6Mariko Sawai7Ryota Uehigashi8Shinji Okada9Naoko Goto-Inoue10Mizuki Morisasa11Taro Kishida12The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanThe United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanDepartment of Applied Biological Chemistry, School of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya 321-8505, JapanPhysiology of Domestic Animals, Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki 036-8561, JapanLaboratory of Food Chemistry, Yokohama University of Pharmacy, Yokohama 245-0066, JapanLaboratory of Nutrition Science, Division of Applied Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanLaboratory of Nutrition Science, Division of Applied Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanLaboratory of Nutrition Science, Division of Applied Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanLaboratory of Nutrition Science, Division of Applied Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanFood Functionality Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa 252-0880, JapanDepartment of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa 252-0880, JapanThe United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, JapanOur previous studies suggested that Alaska pollack protein (APP) intake increases skeletal muscle mass and that it may cause a slow-to-fast shift in muscle fiber type in rats fed a high-fat diet after 56 days of feeding. In this study, we explored whether dietary APP induces acute and sustainable skeletal muscle hypertrophy in rats fed a normal-fat diet. Male 5-week-old Sprague–Dawley rats were divided into four groups and fed a purified ingredient-based high-fat diet or a purified ingredient-based normal-fat diet with casein or APP, containing the same amount of crude protein. Dietary APP significantly increased gastrocnemius muscle mass (105~110%) after 2, 7 days of feeding, regardless of dietary fat content. Rats were separated into two groups and fed a normal-fat diet with casein or APP. Dietary APP significantly increased gastrocnemius muscle mass (110%) after 56 days of feeding. Dietary APP significantly increased the cross-sectional area of the gastrocnemius skeletal muscle and collagen-rich connective tissue after 7 days of feeding. It decreased the gene expression of <i>Mstn</i> /Myostatin, <i>Trim63</i>/MuRF1, and <i>Fbxo32</i>/atrogin-1, but not other gene expression, such as serum IGF-1 after 7 days of feeding. No differences were observed between casein and APP groups with respect to the percentage of Type I, Type IIA, and Type IIX or IIB fibers, as determined by myosin ATPase staining after 7 days of feeding. In the similar experiment, the puromycin-labeled peptides were not different between dietary casein and APP after 2 days of feeding. These results demonstrate that APP induces acute and sustainable skeletal muscle hypertrophy in rats, regardless of dietary fat content. Dietary APP, as a daily protein source, may be an approach for maintaining or increasing muscle mass.https://www.mdpi.com/2072-6643/14/3/547fish proteinskeletal musclemuscle fiber typeprotein sourcerat |
| spellingShingle | Kenji Uchida Mina Fujitani Takafumi Mizushige Fuminori Kawabata Kohsuke Hayamizu Keisuke Uozumi Yuma Hara Mariko Sawai Ryota Uehigashi Shinji Okada Naoko Goto-Inoue Mizuki Morisasa Taro Kishida Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats Nutrients fish protein skeletal muscle muscle fiber type protein source rat |
| title | Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats |
| title_full | Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats |
| title_fullStr | Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats |
| title_full_unstemmed | Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats |
| title_short | Dietary Alaska Pollack Protein Induces Acute and Sustainable Skeletal Muscle Hypertrophy in Rats |
| title_sort | dietary alaska pollack protein induces acute and sustainable skeletal muscle hypertrophy in rats |
| topic | fish protein skeletal muscle muscle fiber type protein source rat |
| url | https://www.mdpi.com/2072-6643/14/3/547 |
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