Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice.
Abstract Purpose Ovarian tissue cryopreservation and its auto‐transplantation is promising technique in fertility preservation. Longevity of grafted tissue is limited though mechanism of follicle reduction is not fully understood. We evaluated histological alteration of vitrified‐thawed ovarian tiss...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12451 |
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| author | Michio Kitajima Naoko Murakami Yuriko Kitajima Itsuki Kajimura Asako Matsumura Kanako Matsumoto Ayumi Harada Kiyonori Miura |
| author_facet | Michio Kitajima Naoko Murakami Yuriko Kitajima Itsuki Kajimura Asako Matsumura Kanako Matsumoto Ayumi Harada Kiyonori Miura |
| author_sort | Michio Kitajima |
| collection | DOAJ |
| description | Abstract Purpose Ovarian tissue cryopreservation and its auto‐transplantation is promising technique in fertility preservation. Longevity of grafted tissue is limited though mechanism of follicle reduction is not fully understood. We evaluated histological alteration of vitrified‐thawed ovarian tissue that grafted to nude mice. Materials and Methods Human ovarian tissue was cryopreserved by vitrification. After thawing, they were grafted to mesentery of nude mice. Twelve weeks after transplantation, the implants were removed and histologically examined. The presence of follicles, the degree of fibrosis, and TUNEL staining in surrounding cortex were evaluated. The stromal expressions of alpha‐smooth muscle actin (aSMA) were determined. Results Normal ovarian cortex was decreased, and fibrotic area were significantly increased after grafting. The distributions of developmental stage of follicles shifted toward activation of follicular growth. Stromal TUNEL staining was increased in frozen/thawed tissue. The expression of aSMA were found in perifollicular stroma of growing follicles, which were decreased in grafted tissue associated with reduction of cortical stroma. Conclusions Fibrosis, reduced cortical stroma, and activation of dormant follicles were concomitantly observed in grafted ovarian cortex, which may relate to limited longevity. Perifollicular aSMA expression can be regarded as a marker of the competence of cortical stroma that regulate follicular development. |
| first_indexed | 2024-04-11T04:44:18Z |
| format | Article |
| id | doaj.art-41810733b2a446bab22d87d32a8dbd2b |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-04-11T04:44:18Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-41810733b2a446bab22d87d32a8dbd2b2022-12-27T13:54:58ZengWileyReproductive Medicine and Biology1445-57811447-05782022-01-01211n/an/a10.1002/rmb2.12451Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice.Michio Kitajima0Naoko Murakami1Yuriko Kitajima2Itsuki Kajimura3Asako Matsumura4Kanako Matsumoto5Ayumi Harada6Kiyonori Miura7Department of Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Hospital Nagasaki JapanDepartment of Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences Nagasaki JapanAbstract Purpose Ovarian tissue cryopreservation and its auto‐transplantation is promising technique in fertility preservation. Longevity of grafted tissue is limited though mechanism of follicle reduction is not fully understood. We evaluated histological alteration of vitrified‐thawed ovarian tissue that grafted to nude mice. Materials and Methods Human ovarian tissue was cryopreserved by vitrification. After thawing, they were grafted to mesentery of nude mice. Twelve weeks after transplantation, the implants were removed and histologically examined. The presence of follicles, the degree of fibrosis, and TUNEL staining in surrounding cortex were evaluated. The stromal expressions of alpha‐smooth muscle actin (aSMA) were determined. Results Normal ovarian cortex was decreased, and fibrotic area were significantly increased after grafting. The distributions of developmental stage of follicles shifted toward activation of follicular growth. Stromal TUNEL staining was increased in frozen/thawed tissue. The expression of aSMA were found in perifollicular stroma of growing follicles, which were decreased in grafted tissue associated with reduction of cortical stroma. Conclusions Fibrosis, reduced cortical stroma, and activation of dormant follicles were concomitantly observed in grafted ovarian cortex, which may relate to limited longevity. Perifollicular aSMA expression can be regarded as a marker of the competence of cortical stroma that regulate follicular development.https://doi.org/10.1002/rmb2.12451alpha‐smooth muscle actinfibrosisfollicular growthovarian cortical stromaovarian tissue cryopreservation |
| spellingShingle | Michio Kitajima Naoko Murakami Yuriko Kitajima Itsuki Kajimura Asako Matsumura Kanako Matsumoto Ayumi Harada Kiyonori Miura Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. Reproductive Medicine and Biology alpha‐smooth muscle actin fibrosis follicular growth ovarian cortical stroma ovarian tissue cryopreservation |
| title | Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. |
| title_full | Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. |
| title_fullStr | Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. |
| title_full_unstemmed | Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. |
| title_short | Accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified‐thawed human ovarian tissue xenografted to nude mice. |
| title_sort | accumulation of fibrosis and altered perifollicular stromal differentiation in vitrified thawed human ovarian tissue xenografted to nude mice |
| topic | alpha‐smooth muscle actin fibrosis follicular growth ovarian cortical stroma ovarian tissue cryopreservation |
| url | https://doi.org/10.1002/rmb2.12451 |
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