Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases
Abstract Background Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1213-9 |
| _version_ | 1819074497645379584 |
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| author | Masood Abu-Halima Martin Poryo Nicole Ludwig Janine Mark Ina Marsollek Christian Giebels Johannes Petersen Hans-Joachim Schäfers Ulrich Grundmann Thomas Pickardt Andreas Keller Eckart Meese Hashim Abdul-Khaliq |
| author_facet | Masood Abu-Halima Martin Poryo Nicole Ludwig Janine Mark Ina Marsollek Christian Giebels Johannes Petersen Hans-Joachim Schäfers Ulrich Grundmann Thomas Pickardt Andreas Keller Eckart Meese Hashim Abdul-Khaliq |
| author_sort | Masood Abu-Halima |
| collection | DOAJ |
| description | Abstract Background Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB. Methods In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools. Results According to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system. Conclusion Our result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular. |
| first_indexed | 2024-12-21T18:10:28Z |
| format | Article |
| id | doaj.art-418218abdadc4938a5dc923c20fab191 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-21T18:10:28Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-418218abdadc4938a5dc923c20fab1912022-12-21T18:54:48ZengBMCJournal of Translational Medicine1479-58762017-05-0115111410.1186/s12967-017-1213-9Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseasesMasood Abu-Halima0Martin Poryo1Nicole Ludwig2Janine Mark3Ina Marsollek4Christian Giebels5Johannes Petersen6Hans-Joachim Schäfers7Ulrich Grundmann8Thomas Pickardt9Andreas Keller10Eckart Meese11Hashim Abdul-Khaliq12Department of Human Genetics, Saarland UniversityDepartment of Pediatric Cardiology, Saarland University Medical CenterDepartment of Human Genetics, Saarland UniversityDepartment of Pediatric Cardiology, Saarland University Medical CenterDepartment of Pediatric Cardiology, Saarland University Medical CenterDepartment of Thoracic and Cardiovascular Surgery, Saarland University Medical CenterDepartment of Pediatric Cardiology, Saarland University Medical CenterDepartment of Thoracic and Cardiovascular Surgery, Saarland University Medical CenterDepartment of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical CenterCompetence Network for Congenital Heart Defects, National Register for Congenital Heart Defects, DZHKDepartment of Clinical Bioinformatics, Saarland UniversityDepartment of Human Genetics, Saarland UniversityDepartment of Pediatric Cardiology, Saarland University Medical CenterAbstract Background Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB. Methods In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools. Results According to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system. Conclusion Our result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular.http://link.springer.com/article/10.1186/s12967-017-1213-9MicroRNACongenital heart diseaseAtrial myocardiumCardiopulmonary bypass |
| spellingShingle | Masood Abu-Halima Martin Poryo Nicole Ludwig Janine Mark Ina Marsollek Christian Giebels Johannes Petersen Hans-Joachim Schäfers Ulrich Grundmann Thomas Pickardt Andreas Keller Eckart Meese Hashim Abdul-Khaliq Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases Journal of Translational Medicine MicroRNA Congenital heart disease Atrial myocardium Cardiopulmonary bypass |
| title | Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases |
| title_full | Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases |
| title_fullStr | Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases |
| title_full_unstemmed | Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases |
| title_short | Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases |
| title_sort | differential expression of micrornas following cardiopulmonary bypass in children with congenital heart diseases |
| topic | MicroRNA Congenital heart disease Atrial myocardium Cardiopulmonary bypass |
| url | http://link.springer.com/article/10.1186/s12967-017-1213-9 |
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