Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)
Abstract Background Arboviruses are a group of viruses transmitted to vertebrate hosts by certain blood-feeding arthropods. Among urban vectors of arboviruses, mosquitoes of the genus Aedes are the most common. However, other mosquitoes may be susceptible to infection and involved in the transmissio...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-023-05707-2 |
| _version_ | 1797836606456987648 |
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| author | Flávia Barreto De Sousa Juliana Santana de Curcio Lívia do Carmo Silva Diego Michel Fernandes da Silva Silvia Maria Salem-Izacc Carlos Eduardo Anunciação Bergmann Morais Ribeiro Marco Tulio A. Garcia-Zapata Elisângela de Paula Silveira-Lacerda |
| author_facet | Flávia Barreto De Sousa Juliana Santana de Curcio Lívia do Carmo Silva Diego Michel Fernandes da Silva Silvia Maria Salem-Izacc Carlos Eduardo Anunciação Bergmann Morais Ribeiro Marco Tulio A. Garcia-Zapata Elisângela de Paula Silveira-Lacerda |
| author_sort | Flávia Barreto De Sousa |
| collection | DOAJ |
| description | Abstract Background Arboviruses are a group of viruses transmitted to vertebrate hosts by certain blood-feeding arthropods. Among urban vectors of arboviruses, mosquitoes of the genus Aedes are the most common. However, other mosquitoes may be susceptible to infection and involved in the transmission, such as Mansonia spp. Therefore, this study aimed to investigate whether Mansonia humeralis can be infected with the Mayaro virus (MAYV). Methods These insects were collected from 2018 to 2020 in chicken coops of rural communities in Jaci Paraná in Porto Velho, Rondônia, Brazil, while performing blood-feeding on roosters. The mosquitoes were randomly grouped in pools from which the head and thorax were macerated and checked for the presence of MAYV by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The positive pools were used to infect the C6/36 cell line, and on different days post-infection, the supernatant of the infected cells was subjected to viral detection by RT-qPCR. Results A total of 183 pools of female mosquitoes were tested, of which 18% were positive for MAYV; some samples from insect pools inoculated into C6/36 cells showed in vitro multiplication capacity between 3 and 7 days post-infection. Conclusions This is the first report of Ma. humeralis mosquitoes that are naturally infected by MAYV, indicating that these vectors may be potential transmitting agents of this arbovirus. Graphical Abstract |
| first_indexed | 2024-04-09T15:11:38Z |
| format | Article |
| id | doaj.art-41838864a57f46d9908aeb04e78fb7a1 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-09T15:11:38Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-41838864a57f46d9908aeb04e78fb7a12023-04-30T11:08:56ZengBMCParasites & Vectors1756-33052023-04-011611610.1186/s13071-023-05707-2Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)Flávia Barreto De Sousa0Juliana Santana de Curcio1Lívia do Carmo Silva2Diego Michel Fernandes da Silva3Silvia Maria Salem-Izacc4Carlos Eduardo Anunciação5Bergmann Morais Ribeiro6Marco Tulio A. Garcia-Zapata7Elisângela de Paula Silveira-Lacerda8Unidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUniversidade de BrasíliaUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBUnidade Sentinela e Centro de Referência em Medicina Internacional e de Viagens (USCREMIVI)/Núcleo de Estudos e Pesquisa de Agentes (Re) Emergentes (NUPEREME), Universidade Federal de Goiás, IPTSP/ICBAbstract Background Arboviruses are a group of viruses transmitted to vertebrate hosts by certain blood-feeding arthropods. Among urban vectors of arboviruses, mosquitoes of the genus Aedes are the most common. However, other mosquitoes may be susceptible to infection and involved in the transmission, such as Mansonia spp. Therefore, this study aimed to investigate whether Mansonia humeralis can be infected with the Mayaro virus (MAYV). Methods These insects were collected from 2018 to 2020 in chicken coops of rural communities in Jaci Paraná in Porto Velho, Rondônia, Brazil, while performing blood-feeding on roosters. The mosquitoes were randomly grouped in pools from which the head and thorax were macerated and checked for the presence of MAYV by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The positive pools were used to infect the C6/36 cell line, and on different days post-infection, the supernatant of the infected cells was subjected to viral detection by RT-qPCR. Results A total of 183 pools of female mosquitoes were tested, of which 18% were positive for MAYV; some samples from insect pools inoculated into C6/36 cells showed in vitro multiplication capacity between 3 and 7 days post-infection. Conclusions This is the first report of Ma. humeralis mosquitoes that are naturally infected by MAYV, indicating that these vectors may be potential transmitting agents of this arbovirus. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05707-2ArbovirusesMansonia humeralisMayaro feverViral isolation |
| spellingShingle | Flávia Barreto De Sousa Juliana Santana de Curcio Lívia do Carmo Silva Diego Michel Fernandes da Silva Silvia Maria Salem-Izacc Carlos Eduardo Anunciação Bergmann Morais Ribeiro Marco Tulio A. Garcia-Zapata Elisângela de Paula Silveira-Lacerda Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) Parasites & Vectors Arboviruses Mansonia humeralis Mayaro fever Viral isolation |
| title | Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) |
| title_full | Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) |
| title_fullStr | Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) |
| title_full_unstemmed | Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) |
| title_short | Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae) |
| title_sort | report of natural mayaro virus infection in mansonia humeralis dyar knab diptera culicidae |
| topic | Arboviruses Mansonia humeralis Mayaro fever Viral isolation |
| url | https://doi.org/10.1186/s13071-023-05707-2 |
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