Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens
Activation of self-reactive CD8+ T cells induces a peripheral tolerance mechanism that involves loss of CD8 expression. Because genetic deficiency of Fas and Fasl causes the accumulation of double-negative (DN; CD3+ TCR-αβ+ CD4- CD8-) T cells that have been proposed to derive from CD8+ cells, we dec...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.635862/full |
| _version_ | 1818576395044913152 |
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| author | Giovanna Flores-Mendoza Giovanna Flores-Mendoza Noé Rodríguez-Rodríguez Rosa M. Rubio Iris K. Madera-Salcedo Florencia Rosetti José C. Crispín José C. Crispín |
| author_facet | Giovanna Flores-Mendoza Giovanna Flores-Mendoza Noé Rodríguez-Rodríguez Rosa M. Rubio Iris K. Madera-Salcedo Florencia Rosetti José C. Crispín José C. Crispín |
| author_sort | Giovanna Flores-Mendoza |
| collection | DOAJ |
| description | Activation of self-reactive CD8+ T cells induces a peripheral tolerance mechanism that involves loss of CD8 expression. Because genetic deficiency of Fas and Fasl causes the accumulation of double-negative (DN; CD3+ TCR-αβ+ CD4- CD8-) T cells that have been proposed to derive from CD8+ cells, we decided to explore the role of Fas and FasL in self-antigen-induced CD8 downregulation. To this end, we quantified Fas and FasL induction by different stimuli and analyzed the effects of Fas/FasL deficiency during a protective immune response and after exposure to self-antigens. Our data describes how Fas and FasL upregulation differs depending on the setting of CD8 T cell activation and demonstrates that Fas/FasL signaling maintains CD8 expression during repetitive antigen stimulation and following self-antigen encounter. Together, our results reveal an unexpected role of Fas/FasL signaling and offer a new insight into the role of these molecules in the regulation of immune tolerance. |
| first_indexed | 2024-12-16T06:13:20Z |
| format | Article |
| id | doaj.art-4184c604a5894975b0755b21e4f98663 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T06:13:20Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4184c604a5894975b0755b21e4f986632022-12-21T22:41:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-03-011210.3389/fimmu.2021.635862635862Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-AntigensGiovanna Flores-Mendoza0Giovanna Flores-Mendoza1Noé Rodríguez-Rodríguez2Rosa M. Rubio3Iris K. Madera-Salcedo4Florencia Rosetti5José C. Crispín6José C. Crispín7Departamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoPosgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México (UNAM), Mexico City, MexicoDepartamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoDepartamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoDepartamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoDepartamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoDepartamento de Inmunología y Reumatología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, MexicoEscuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, Monterrey, MexicoActivation of self-reactive CD8+ T cells induces a peripheral tolerance mechanism that involves loss of CD8 expression. Because genetic deficiency of Fas and Fasl causes the accumulation of double-negative (DN; CD3+ TCR-αβ+ CD4- CD8-) T cells that have been proposed to derive from CD8+ cells, we decided to explore the role of Fas and FasL in self-antigen-induced CD8 downregulation. To this end, we quantified Fas and FasL induction by different stimuli and analyzed the effects of Fas/FasL deficiency during a protective immune response and after exposure to self-antigens. Our data describes how Fas and FasL upregulation differs depending on the setting of CD8 T cell activation and demonstrates that Fas/FasL signaling maintains CD8 expression during repetitive antigen stimulation and following self-antigen encounter. Together, our results reveal an unexpected role of Fas/FasL signaling and offer a new insight into the role of these molecules in the regulation of immune tolerance.https://www.frontiersin.org/articles/10.3389/fimmu.2021.635862/fullCD8CD95FasFasLtolerancedouble negative T cell |
| spellingShingle | Giovanna Flores-Mendoza Giovanna Flores-Mendoza Noé Rodríguez-Rodríguez Rosa M. Rubio Iris K. Madera-Salcedo Florencia Rosetti José C. Crispín José C. Crispín Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens Frontiers in Immunology CD8 CD95 Fas FasL tolerance double negative T cell |
| title | Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens |
| title_full | Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens |
| title_fullStr | Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens |
| title_full_unstemmed | Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens |
| title_short | Fas/FasL Signaling Regulates CD8 Expression During Exposure to Self-Antigens |
| title_sort | fas fasl signaling regulates cd8 expression during exposure to self antigens |
| topic | CD8 CD95 Fas FasL tolerance double negative T cell |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.635862/full |
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