MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF
Background: MicroRNAs (miRNAs) play an important regulatory role in carcinogenesis and cancer progression. MiR-95-3p has been reported to be an oncogene in hepatocellular carcinoma. However, the role of miR-95-3p in colorectal carcinoma (CRC) remains unclear. Methods: miR-95-3p was validated in an i...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-04-01
|
| Series: | Biomedical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2319417018304931 |
| _version_ | 1811186728415789056 |
|---|---|
| author | Yong-gang Hong Zhi-ping Huang Qi-zhi Liu Ji-Fu E Xian-hua Gao Cheng Xin Wei Zhang Pengpeng Li Li-qiang Hao |
| author_facet | Yong-gang Hong Zhi-ping Huang Qi-zhi Liu Ji-Fu E Xian-hua Gao Cheng Xin Wei Zhang Pengpeng Li Li-qiang Hao |
| author_sort | Yong-gang Hong |
| collection | DOAJ |
| description | Background: MicroRNAs (miRNAs) play an important regulatory role in carcinogenesis and cancer progression. MiR-95-3p has been reported to be an oncogene in hepatocellular carcinoma. However, the role of miR-95-3p in colorectal carcinoma (CRC) remains unclear. Methods: miR-95-3p was validated in an independent validation sample cohort of 215 CRC tissues. Functional assays, Cell proliferation (MTT) assay colony formation, wound healing, transwell and animal xenograft assays were used to determine the oppressor role of miR-95-3p in human CRC progression. Furthermore, Bioinformatics analysis, western blotting and dual-luciferase reporter assay were used to determine the mechanism by which miR-95-3p suppresses progression of CRC cells. Results: In this study, we found that miR-95-3p was downregulated in CRC tissues. The low level of miR-95-3p in CRC tumors was correlated with aggressive clinicopathological characteristics, and it predicted poor prognosis in CRC patients. The overexpression of miR-95-3p significantly inhibited CRC cell proliferation, colony formation and metastasis in vitro and in vivo. Bioinformatic analysis further identified hepatoma-derived growth factor (HDGF) as a novel target of miR-95-3p in CRC cells. These findings suggest that miR-95-3p regulates CRC cell survival, partially through the downregulation of HDGF. Conclusions: Therefore, the miR-95-3p/HDGF axis might serve as a novel therapeutic target in patients with CRC. |
| first_indexed | 2024-04-11T13:50:25Z |
| format | Article |
| id | doaj.art-4185eefacb764f3da7b8de2d7cc18cdc |
| institution | Directory Open Access Journal |
| issn | 2319-4170 |
| language | English |
| last_indexed | 2024-04-11T13:50:25Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Journal |
| spelling | doaj.art-4185eefacb764f3da7b8de2d7cc18cdc2022-12-22T04:20:38ZengElsevierBiomedical Journal2319-41702020-04-01432163173MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGFYong-gang Hong0Zhi-ping Huang1Qi-zhi Liu2Ji-Fu E3Xian-hua Gao4Cheng Xin5Wei Zhang6Pengpeng Li7Li-qiang Hao8Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, ChinaThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China; The General Hospital of Guangzhou Military Command, Guangzhou, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China; Corresponding author. Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Rd, Shanghai, 200433, ChinaThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China; Corresponding author. Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, 168 Changhai Rd, Shanghai, 200433, ChinaBackground: MicroRNAs (miRNAs) play an important regulatory role in carcinogenesis and cancer progression. MiR-95-3p has been reported to be an oncogene in hepatocellular carcinoma. However, the role of miR-95-3p in colorectal carcinoma (CRC) remains unclear. Methods: miR-95-3p was validated in an independent validation sample cohort of 215 CRC tissues. Functional assays, Cell proliferation (MTT) assay colony formation, wound healing, transwell and animal xenograft assays were used to determine the oppressor role of miR-95-3p in human CRC progression. Furthermore, Bioinformatics analysis, western blotting and dual-luciferase reporter assay were used to determine the mechanism by which miR-95-3p suppresses progression of CRC cells. Results: In this study, we found that miR-95-3p was downregulated in CRC tissues. The low level of miR-95-3p in CRC tumors was correlated with aggressive clinicopathological characteristics, and it predicted poor prognosis in CRC patients. The overexpression of miR-95-3p significantly inhibited CRC cell proliferation, colony formation and metastasis in vitro and in vivo. Bioinformatic analysis further identified hepatoma-derived growth factor (HDGF) as a novel target of miR-95-3p in CRC cells. These findings suggest that miR-95-3p regulates CRC cell survival, partially through the downregulation of HDGF. Conclusions: Therefore, the miR-95-3p/HDGF axis might serve as a novel therapeutic target in patients with CRC.http://www.sciencedirect.com/science/article/pii/S2319417018304931MicroRNA-95-3pHDGFColorectal carcinomaBiomarkerPrognosis |
| spellingShingle | Yong-gang Hong Zhi-ping Huang Qi-zhi Liu Ji-Fu E Xian-hua Gao Cheng Xin Wei Zhang Pengpeng Li Li-qiang Hao MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF Biomedical Journal MicroRNA-95-3p HDGF Colorectal carcinoma Biomarker Prognosis |
| title | MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF |
| title_full | MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF |
| title_fullStr | MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF |
| title_full_unstemmed | MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF |
| title_short | MicroRNA-95-3p inhibits cell proliferation and metastasis in colorectal carcinoma by HDGF |
| title_sort | microrna 95 3p inhibits cell proliferation and metastasis in colorectal carcinoma by hdgf |
| topic | MicroRNA-95-3p HDGF Colorectal carcinoma Biomarker Prognosis |
| url | http://www.sciencedirect.com/science/article/pii/S2319417018304931 |
| work_keys_str_mv | AT yongganghong microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT zhipinghuang microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT qizhiliu microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT jifue microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT xianhuagao microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT chengxin microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT weizhang microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT pengpengli microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf AT liqianghao microrna953pinhibitscellproliferationandmetastasisincolorectalcarcinomabyhdgf |