Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model
The current pandemic caused by severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has encouraged the evaluation of novel instruments for disinfection and lowering infectious pressure. Ultraviolet subtype C (UVC) excimer lamps with 222 nm wavelength have been tested on airborne path...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/9/2038 |
| _version_ | 1827655759501983744 |
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| author | Claudia Maria Tucciarone Mattia Cecchinato Lucrezia Vianello Gabriele Simi Enrico Borsato Luca Silvestrin Michele Giorato Cristiano Salata Mauro Morandin Elisa Greggio Michele Drigo |
| author_facet | Claudia Maria Tucciarone Mattia Cecchinato Lucrezia Vianello Gabriele Simi Enrico Borsato Luca Silvestrin Michele Giorato Cristiano Salata Mauro Morandin Elisa Greggio Michele Drigo |
| author_sort | Claudia Maria Tucciarone |
| collection | DOAJ |
| description | The current pandemic caused by severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has encouraged the evaluation of novel instruments for disinfection and lowering infectious pressure. Ultraviolet subtype C (UVC) excimer lamps with 222 nm wavelength have been tested on airborne pathogens on surfaces and the exposure to this wavelength has been considered safer than conventional UVC. To test the efficacy of UVC excimer lamps on coronaviruses, an animal model mimicking the infection dynamics was implemented. An attenuated vaccine based on infectious bronchitis virus (IBV) was nebulized and irradiated by 222 nm UVC rays before the exposure of a group of day-old chicks to evaluate the virus inactivation. A control group of chicks was exposed to the nebulized vaccine produced in the same conditions but not irradiated by the lamps. The animals of both groups were sampled daily and individually by choanal cleft swabs and tested usign a strain specific real time RT-PCR to evaluate the vaccine replication. Only the birds in the control group were positive, showing an active replication of the vaccine, revealing the efficacy of the lamps in inactivating the vaccine below the infectious dose in the other group. |
| first_indexed | 2024-03-09T22:13:09Z |
| format | Article |
| id | doaj.art-41870363b1a143e9b6134f52cb4f38af |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T22:13:09Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-41870363b1a143e9b6134f52cb4f38af2023-11-23T19:28:33ZengMDPI AGViruses1999-49152022-09-01149203810.3390/v14092038Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal ModelClaudia Maria Tucciarone0Mattia Cecchinato1Lucrezia Vianello2Gabriele Simi3Enrico Borsato4Luca Silvestrin5Michele Giorato6Cristiano Salata7Mauro Morandin8Elisa Greggio9Michele Drigo10Department of Animal Medicine, Production and Health (MAPS), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Animal Medicine, Production and Health (MAPS), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Animal Medicine, Production and Health (MAPS), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Physics and Astronomy (DFA), University of Padova, Via Marzolo 8, 35131 Padova, ItalyDepartment of Physics and Astronomy (DFA), University of Padova, Via Marzolo 8, 35131 Padova, ItalyDepartment of Physics and Astronomy (DFA), University of Padova, Via Marzolo 8, 35131 Padova, ItalyDepartment of Physics and Astronomy (DFA), University of Padova, Via Marzolo 8, 35131 Padova, ItalyDepartment of Molecular Medicine (DMM), University of Padova, Via Gabelli 63, 35121 Padova, ItalyNational Institute for Nuclear Physics (INFN), Padova Division, Via Marzolo 8, 35131 Padova, ItalyDepartment of Biology (DiBio), University of Padova, Viale G. Colombo 3, 35131 Padova, ItalyDepartment of Animal Medicine, Production and Health (MAPS), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyThe current pandemic caused by severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has encouraged the evaluation of novel instruments for disinfection and lowering infectious pressure. Ultraviolet subtype C (UVC) excimer lamps with 222 nm wavelength have been tested on airborne pathogens on surfaces and the exposure to this wavelength has been considered safer than conventional UVC. To test the efficacy of UVC excimer lamps on coronaviruses, an animal model mimicking the infection dynamics was implemented. An attenuated vaccine based on infectious bronchitis virus (IBV) was nebulized and irradiated by 222 nm UVC rays before the exposure of a group of day-old chicks to evaluate the virus inactivation. A control group of chicks was exposed to the nebulized vaccine produced in the same conditions but not irradiated by the lamps. The animals of both groups were sampled daily and individually by choanal cleft swabs and tested usign a strain specific real time RT-PCR to evaluate the vaccine replication. Only the birds in the control group were positive, showing an active replication of the vaccine, revealing the efficacy of the lamps in inactivating the vaccine below the infectious dose in the other group.https://www.mdpi.com/1999-4915/14/9/2038severe acute respiratory syndrome-related coronavirus-2infectious bronchitis virusexcimer lampsUVC222 nmanimal model |
| spellingShingle | Claudia Maria Tucciarone Mattia Cecchinato Lucrezia Vianello Gabriele Simi Enrico Borsato Luca Silvestrin Michele Giorato Cristiano Salata Mauro Morandin Elisa Greggio Michele Drigo Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model Viruses severe acute respiratory syndrome-related coronavirus-2 infectious bronchitis virus excimer lamps UVC 222 nm animal model |
| title | Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model |
| title_full | Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model |
| title_fullStr | Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model |
| title_full_unstemmed | Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model |
| title_short | Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model |
| title_sort | evaluation of uvc excimer lamp 222 nm efficacy for coronavirus inactivation in an animal model |
| topic | severe acute respiratory syndrome-related coronavirus-2 infectious bronchitis virus excimer lamps UVC 222 nm animal model |
| url | https://www.mdpi.com/1999-4915/14/9/2038 |
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