Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge
Abstract Regulatory T cells (Tregs) are found to participate in the pathogenesis of cerebral ischemic stroke. Exosomes derived from Tregs (Treg-Exos) were found to mediate the mechanism of Tregs’ roles under various physiological and pathological conditions. But the roles of Treg-Exos in cerebral is...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2022-12-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500701&tlng=en |
| _version_ | 1811195397702418432 |
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| author | Changqing Yang Fei Yuan Wan Shao Lihong Yao Shaoju Jin Fangfang Han |
| author_facet | Changqing Yang Fei Yuan Wan Shao Lihong Yao Shaoju Jin Fangfang Han |
| author_sort | Changqing Yang |
| collection | DOAJ |
| description | Abstract Regulatory T cells (Tregs) are found to participate in the pathogenesis of cerebral ischemic stroke. Exosomes derived from Tregs (Treg-Exos) were found to mediate the mechanism of Tregs’ roles under various physiological and pathological conditions. But the roles of Treg-Exos in cerebral ischemic stroke are still unclear. Here, we explored the protective effects of Treg-Exos against microglial injury in response to oxygen-glucose deprivation/reperfusion (OGD/R) exposure. The results showed that Tregs-Exos relieved OGD/R-caused increases in LDH release and caspase-3 activity in BV-2 cells. The decreased cell viability and increased percentage of TUNEL-positive cells in OGD/R-exposed BV-2 cells were attenuated by Tregs-Exos treatment. Tregs-Exos also suppressed OGD/R-induced increase in production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in BV-2 microglia. Furthermore, Tregs-Exos induced the expression levels of phosphorylated phosphatidylinositol-3-kinase (p-PI3K) and phosphorylated protein kinase B (p-Akt) in BV-2 microglia under the challenge of OGD/R. Inhibition of the PI3K/Akt signaling by LY294002 partly reversed the effects of Tregs-Exos on cell apoptosis and inflammation in OGD/R-exposed BV-2 microglia. These results indicated that Tregs-Exos exerted protective effects against the OGD/R-caused injury of BV-2 microglia by activating the PI3K/Akt signaling. |
| first_indexed | 2024-04-12T00:42:41Z |
| format | Article |
| id | doaj.art-4187c008487a4cc28137ba4bef9b6f80 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-04-12T00:42:41Z |
| publishDate | 2022-12-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-4187c008487a4cc28137ba4bef9b6f802022-12-22T03:54:58ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852022-12-0145410.1590/1678-4685-gmb-2022-0119Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challengeChangqing YangFei YuanWan ShaoLihong YaoShaoju JinFangfang Hanhttps://orcid.org/0000-0002-8428-114XAbstract Regulatory T cells (Tregs) are found to participate in the pathogenesis of cerebral ischemic stroke. Exosomes derived from Tregs (Treg-Exos) were found to mediate the mechanism of Tregs’ roles under various physiological and pathological conditions. But the roles of Treg-Exos in cerebral ischemic stroke are still unclear. Here, we explored the protective effects of Treg-Exos against microglial injury in response to oxygen-glucose deprivation/reperfusion (OGD/R) exposure. The results showed that Tregs-Exos relieved OGD/R-caused increases in LDH release and caspase-3 activity in BV-2 cells. The decreased cell viability and increased percentage of TUNEL-positive cells in OGD/R-exposed BV-2 cells were attenuated by Tregs-Exos treatment. Tregs-Exos also suppressed OGD/R-induced increase in production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in BV-2 microglia. Furthermore, Tregs-Exos induced the expression levels of phosphorylated phosphatidylinositol-3-kinase (p-PI3K) and phosphorylated protein kinase B (p-Akt) in BV-2 microglia under the challenge of OGD/R. Inhibition of the PI3K/Akt signaling by LY294002 partly reversed the effects of Tregs-Exos on cell apoptosis and inflammation in OGD/R-exposed BV-2 microglia. These results indicated that Tregs-Exos exerted protective effects against the OGD/R-caused injury of BV-2 microglia by activating the PI3K/Akt signaling.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500701&tlng=enRegulatory T cellsexosomesischemic strokemicrogliaphosphatidylinositol-3-kinase/protein kinase B signaling |
| spellingShingle | Changqing Yang Fei Yuan Wan Shao Lihong Yao Shaoju Jin Fangfang Han Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge Genetics and Molecular Biology Regulatory T cells exosomes ischemic stroke microglia phosphatidylinositol-3-kinase/protein kinase B signaling |
| title | Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge |
| title_full | Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge |
| title_fullStr | Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge |
| title_full_unstemmed | Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge |
| title_short | Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge |
| title_sort | protective role of exosomes derived from regulatory t cells against inflammation and apoptosis of bv 2 microglia under oxygen glucose deprivation reperfusion challenge |
| topic | Regulatory T cells exosomes ischemic stroke microglia phosphatidylinositol-3-kinase/protein kinase B signaling |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572022000500701&tlng=en |
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