Neuronal imaging with ultrahigh dynamic range multiphoton microscopy
Abstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-06065-7 |
| _version_ | 1831771955739492352 |
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| author | Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz |
| author_facet | Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz |
| author_sort | Ruohui Yang |
| collection | DOAJ |
| description | Abstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation of the sample strength to accommodate ultrahigh dynamic range without loss of information. No microscope hardware modifications are required, making the technique readily compatible with commercial instruments. Benefits are shown in both structural and in-vivo functional mouse brain imaging applications. |
| first_indexed | 2024-12-22T08:08:48Z |
| format | Article |
| id | doaj.art-418a4726ec0748a2a28bf1864c3ab081 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T08:08:48Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-418a4726ec0748a2a28bf1864c3ab0812022-12-21T18:33:04ZengNature PortfolioScientific Reports2045-23222017-07-01711710.1038/s41598-017-06065-7Neuronal imaging with ultrahigh dynamic range multiphoton microscopyRuohui Yang0Timothy D. Weber1Ellen D. Witkowski2Ian G. Davison3Jerome Mertz4Boston University Department of Biomedical EngineeringBoston University Department of Biomedical EngineeringBoston University Department of BiologyBoston University Department of BiologyBoston University Department of Biomedical EngineeringAbstract Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation of the sample strength to accommodate ultrahigh dynamic range without loss of information. No microscope hardware modifications are required, making the technique readily compatible with commercial instruments. Benefits are shown in both structural and in-vivo functional mouse brain imaging applications.https://doi.org/10.1038/s41598-017-06065-7 |
| spellingShingle | Ruohui Yang Timothy D. Weber Ellen D. Witkowski Ian G. Davison Jerome Mertz Neuronal imaging with ultrahigh dynamic range multiphoton microscopy Scientific Reports |
| title | Neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| title_full | Neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| title_fullStr | Neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| title_full_unstemmed | Neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| title_short | Neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| title_sort | neuronal imaging with ultrahigh dynamic range multiphoton microscopy |
| url | https://doi.org/10.1038/s41598-017-06065-7 |
| work_keys_str_mv | AT ruohuiyang neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT timothydweber neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT ellendwitkowski neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT iangdavison neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy AT jeromemertz neuronalimagingwithultrahighdynamicrangemultiphotonmicroscopy |