Human iPSC-derived glia models for the study of neuroinflammation
Abstract Neuroinflammation is a complex biological process that plays a significant role in various brain disorders. Microglia and astrocytes are the key cell types involved in inflammatory responses in the central nervous system. Neuroinflammation results in increased levels of secreted inflammator...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12974-023-02919-2 |
| _version_ | 1797451956315226112 |
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| author | Nina Stöberl Emily Maguire Elisa Salis Bethany Shaw Hazel Hall-Roberts |
| author_facet | Nina Stöberl Emily Maguire Elisa Salis Bethany Shaw Hazel Hall-Roberts |
| author_sort | Nina Stöberl |
| collection | DOAJ |
| description | Abstract Neuroinflammation is a complex biological process that plays a significant role in various brain disorders. Microglia and astrocytes are the key cell types involved in inflammatory responses in the central nervous system. Neuroinflammation results in increased levels of secreted inflammatory factors, such as cytokines, chemokines, and reactive oxygen species. To model neuroinflammation in vitro, various human induced pluripotent stem cell (iPSC)-based models have been utilized, including monocultures, transfer of conditioned media between cell types, co-culturing multiple cell types, neural organoids, and xenotransplantation of cells into the mouse brain. To induce neuroinflammatory responses in vitro, several stimuli have been established that can induce responses in either microglia, astrocytes, or both. Here, we describe and critically evaluate the different types of iPSC models that can be used to study neuroinflammation and highlight how neuroinflammation has been induced and measured in these cultures. |
| first_indexed | 2024-03-09T15:01:53Z |
| format | Article |
| id | doaj.art-418d2a3c62104723a3b392675a6920c8 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-03-09T15:01:53Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-418d2a3c62104723a3b392675a6920c82023-11-26T13:51:48ZengBMCJournal of Neuroinflammation1742-20942023-10-0120112010.1186/s12974-023-02919-2Human iPSC-derived glia models for the study of neuroinflammationNina Stöberl0Emily Maguire1Elisa Salis2Bethany Shaw3Hazel Hall-Roberts4UK Dementia Research Institute (UK DRI), School of Medicine, Cardiff UniversityUK Dementia Research Institute (UK DRI), School of Medicine, Cardiff UniversityUK Dementia Research Institute (UK DRI), School of Medicine, Cardiff UniversityUK Dementia Research Institute (UK DRI), School of Medicine, Cardiff UniversityUK Dementia Research Institute (UK DRI), School of Medicine, Cardiff UniversityAbstract Neuroinflammation is a complex biological process that plays a significant role in various brain disorders. Microglia and astrocytes are the key cell types involved in inflammatory responses in the central nervous system. Neuroinflammation results in increased levels of secreted inflammatory factors, such as cytokines, chemokines, and reactive oxygen species. To model neuroinflammation in vitro, various human induced pluripotent stem cell (iPSC)-based models have been utilized, including monocultures, transfer of conditioned media between cell types, co-culturing multiple cell types, neural organoids, and xenotransplantation of cells into the mouse brain. To induce neuroinflammatory responses in vitro, several stimuli have been established that can induce responses in either microglia, astrocytes, or both. Here, we describe and critically evaluate the different types of iPSC models that can be used to study neuroinflammation and highlight how neuroinflammation has been induced and measured in these cultures.https://doi.org/10.1186/s12974-023-02919-2Induced pluripotent stem cellsiPSCNeuroinflammationMicrogliaAstrocytesMonoculture |
| spellingShingle | Nina Stöberl Emily Maguire Elisa Salis Bethany Shaw Hazel Hall-Roberts Human iPSC-derived glia models for the study of neuroinflammation Journal of Neuroinflammation Induced pluripotent stem cells iPSC Neuroinflammation Microglia Astrocytes Monoculture |
| title | Human iPSC-derived glia models for the study of neuroinflammation |
| title_full | Human iPSC-derived glia models for the study of neuroinflammation |
| title_fullStr | Human iPSC-derived glia models for the study of neuroinflammation |
| title_full_unstemmed | Human iPSC-derived glia models for the study of neuroinflammation |
| title_short | Human iPSC-derived glia models for the study of neuroinflammation |
| title_sort | human ipsc derived glia models for the study of neuroinflammation |
| topic | Induced pluripotent stem cells iPSC Neuroinflammation Microglia Astrocytes Monoculture |
| url | https://doi.org/10.1186/s12974-023-02919-2 |
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