Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles
Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively u...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/4/502 |
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| author | Haya A. Alfassam Majed S. Nassar Manal M. Almusaynid Bashayer A. Khalifah Abdullah S. Alshahrani Fahad A. Almughem Abdullah A. Alshehri Majed O. Alawad Salam Massadeh Manal Alaamery Ibrahim M. Aldeailej Aref A. Alamri Abdulwahab Z. Binjomah Essam A. Tawfik |
| author_facet | Haya A. Alfassam Majed S. Nassar Manal M. Almusaynid Bashayer A. Khalifah Abdullah S. Alshahrani Fahad A. Almughem Abdullah A. Alshehri Majed O. Alawad Salam Massadeh Manal Alaamery Ibrahim M. Aldeailej Aref A. Alamri Abdulwahab Z. Binjomah Essam A. Tawfik |
| author_sort | Haya A. Alfassam |
| collection | DOAJ |
| description | Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as ‘surface plasmonic resonance’ in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients’ swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (−30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients’ swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention. |
| first_indexed | 2024-03-10T12:33:42Z |
| format | Article |
| id | doaj.art-418ea6da94d646c39320ed56c25491a6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T12:33:42Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-418ea6da94d646c39320ed56c25491a62023-11-21T14:26:20ZengMDPI AGPharmaceutics1999-49232021-04-0113450210.3390/pharmaceutics13040502Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold NanoparticlesHaya A. Alfassam0Majed S. Nassar1Manal M. Almusaynid2Bashayer A. Khalifah3Abdullah S. Alshahrani4Fahad A. Almughem5Abdullah A. Alshehri6Majed O. Alawad7Salam Massadeh8Manal Alaamery9Ibrahim M. Aldeailej10Aref A. Alamri11Abdulwahab Z. Binjomah12Essam A. Tawfik13KACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaNational Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaNational Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaNational Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaMicrobiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi ArabiaNational Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaNational Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaMaterial Science Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaKACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaKACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaMicrobiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi ArabiaMicrobiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi ArabiaMicrobiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi ArabiaKACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaSialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as ‘surface plasmonic resonance’ in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients’ swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (−30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients’ swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention.https://www.mdpi.com/1999-4923/13/4/502gold nanoparticlessialic acidhemagglutininsurface plasmonic resonanceviral detectioncolorimetric tool |
| spellingShingle | Haya A. Alfassam Majed S. Nassar Manal M. Almusaynid Bashayer A. Khalifah Abdullah S. Alshahrani Fahad A. Almughem Abdullah A. Alshehri Majed O. Alawad Salam Massadeh Manal Alaamery Ibrahim M. Aldeailej Aref A. Alamri Abdulwahab Z. Binjomah Essam A. Tawfik Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles Pharmaceutics gold nanoparticles sialic acid hemagglutinin surface plasmonic resonance viral detection colorimetric tool |
| title | Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles |
| title_full | Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles |
| title_fullStr | Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles |
| title_full_unstemmed | Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles |
| title_short | Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles |
| title_sort | development of a colorimetric tool for sars cov 2 and other respiratory viruses detection using sialic acid fabricated gold nanoparticles |
| topic | gold nanoparticles sialic acid hemagglutinin surface plasmonic resonance viral detection colorimetric tool |
| url | https://www.mdpi.com/1999-4923/13/4/502 |
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