Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices
Abstract The existing HPLC methods for the assaying of multiple markers in standardized herbal extracts use a single wavelength for quantification, which usually leads to lower assay than actual claims. It was because screening multiple phytochemical standards irrespective to their absorption maxima...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-11-01
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| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43094-023-00555-4 |
| _version_ | 1797577579542085632 |
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| author | Gnanabhaskar Danaboina Rudramani Tiwari Kurra Subrahmanyam V. Shreekala Ajay Kumar Meena |
| author_facet | Gnanabhaskar Danaboina Rudramani Tiwari Kurra Subrahmanyam V. Shreekala Ajay Kumar Meena |
| author_sort | Gnanabhaskar Danaboina |
| collection | DOAJ |
| description | Abstract The existing HPLC methods for the assaying of multiple markers in standardized herbal extracts use a single wavelength for quantification, which usually leads to lower assay than actual claims. It was because screening multiple phytochemical standards irrespective to their absorption maxima which drive decrease in peak are response which ultimately impact the assay. To overcome this issue, we have applied a methodology, called Mixed Standard Multi-Signal (MSMS) simultaneous detection, where multiple reference standards get extracted each with their respective λ max and quantification will be done at the same λ max in a single sequence data setup. This method was standardized using Single Signal Multi-Standards, Single Signal Single Standard, Multi-Signal Single Standard, for all individual reference standards, mixed reference standards, plant extracts, and Ayurvedic formulations. The results of our study demonstrate that higher assay reported total actives 5.04% by conventional detection and for current proposed method 13.81% with shorter span of analysis time and lesser solvent consumption. It helps to decide the actual standardization label claim on product which drives better commercial costings, and dosage regimen claims. Graphical Abstract |
| first_indexed | 2024-03-10T22:11:14Z |
| format | Article |
| id | doaj.art-4194342131e846049a928a76744dd293 |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-03-10T22:11:14Z |
| publishDate | 2023-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-4194342131e846049a928a76744dd2932023-11-19T12:36:41ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532023-11-019111210.1186/s43094-023-00555-4Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matricesGnanabhaskar Danaboina0Rudramani Tiwari1Kurra Subrahmanyam2V. Shreekala3Ajay Kumar Meena4Department of Chemistry, Regional Ayurveda Research Institute, Ministry of Ayush, Govt. of IndiaDepartment of Chemistry, Regional Ayurveda Research Institute, Ministry of Ayush, Govt. of IndiaDepartment of Pharmacology, Regional Ayurveda Research InstituteDepartment of Ayurveda, Regional Ayurveda Research InstituteDepartment of Chemistry, Regional Ayurveda Research Institute, Ministry of Ayush, Govt. of IndiaAbstract The existing HPLC methods for the assaying of multiple markers in standardized herbal extracts use a single wavelength for quantification, which usually leads to lower assay than actual claims. It was because screening multiple phytochemical standards irrespective to their absorption maxima which drive decrease in peak are response which ultimately impact the assay. To overcome this issue, we have applied a methodology, called Mixed Standard Multi-Signal (MSMS) simultaneous detection, where multiple reference standards get extracted each with their respective λ max and quantification will be done at the same λ max in a single sequence data setup. This method was standardized using Single Signal Multi-Standards, Single Signal Single Standard, Multi-Signal Single Standard, for all individual reference standards, mixed reference standards, plant extracts, and Ayurvedic formulations. The results of our study demonstrate that higher assay reported total actives 5.04% by conventional detection and for current proposed method 13.81% with shorter span of analysis time and lesser solvent consumption. It helps to decide the actual standardization label claim on product which drives better commercial costings, and dosage regimen claims. Graphical Abstracthttps://doi.org/10.1186/s43094-023-00555-4HPLCDADMSMSSimultaneous detectionBioactivesFood |
| spellingShingle | Gnanabhaskar Danaboina Rudramani Tiwari Kurra Subrahmanyam V. Shreekala Ajay Kumar Meena Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices Future Journal of Pharmaceutical Sciences HPLC DAD MSMS Simultaneous detection Bioactives Food |
| title | Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| title_full | Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| title_fullStr | Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| title_full_unstemmed | Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| title_short | Multi-wavelength fusion spectral extraction-assisted HPLC for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| title_sort | multi wavelength fusion spectral extraction assisted hplc for simultaneous quantification of multiple secondary metabolites in herbal matrices |
| topic | HPLC DAD MSMS Simultaneous detection Bioactives Food |
| url | https://doi.org/10.1186/s43094-023-00555-4 |
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