MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava
Cassava, being one of the top three tuberous crops, features highly efficient starch accumulation in the storage root to adapt the tropical resources and environments. The molecular mechanism for the process, however, is still unclear. ADP-glucose pyrophosphorylase, the first and rate-limited enzyme...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01315/full |
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| author | Ping’an Ma Ping’an Ma Ping’an Ma Xin Chen Xin Chen Chen Liu Chen Liu Chen Liu Yuhong Meng Yuhong Meng Yuhong Meng Zhiqiang Xia Zhiqiang Xia Changying Zeng Changying Zeng Cheng Lu Cheng Lu Wenquan Wang Wenquan Wang |
| author_facet | Ping’an Ma Ping’an Ma Ping’an Ma Xin Chen Xin Chen Chen Liu Chen Liu Chen Liu Yuhong Meng Yuhong Meng Yuhong Meng Zhiqiang Xia Zhiqiang Xia Changying Zeng Changying Zeng Cheng Lu Cheng Lu Wenquan Wang Wenquan Wang |
| author_sort | Ping’an Ma |
| collection | DOAJ |
| description | Cassava, being one of the top three tuberous crops, features highly efficient starch accumulation in the storage root to adapt the tropical resources and environments. The molecular mechanism for the process, however, is still unclear. ADP-glucose pyrophosphorylase, the first and rate-limited enzyme in starch biosynthesis pathway, is a heterotetramer comprised of two small/catalytic and two large/modulatory subunits. To understand the regulation of MeAGPase, the promoter of a highly expressed small subunit, MeAGPs1a, was used as bait for a yeast one-hybrid assay to screen storage root cDNA library. One cDNA, coding for a small auxin-up RNA protein, named MeSAUR1, was isolated from cassava. MeSAUR1 could bind to the promoter of MeAGPS1a in yeast one-hybrid test and in vitro, and was located in cell nucleus. MeSAUR1 displayed a higher transcript level in cassava root cortex, and its expression was induced by indole-3-acetic acid, gibberellin and ethylene, but repressed by abscisic acid. A dual-luciferase interaction test further convinced that MeSAUR1 could bind to the promoter of MeAGPS1a, and positively regulate the transcription of MeAGPS1a in cassava. |
| first_indexed | 2024-12-24T03:49:31Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-24T03:49:31Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-419554879661423f9cab97893cd9f0a52022-12-21T17:16:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01315277930MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in CassavaPing’an Ma0Ping’an Ma1Ping’an Ma2Xin Chen3Xin Chen4Chen Liu5Chen Liu6Chen Liu7Yuhong Meng8Yuhong Meng9Yuhong Meng10Zhiqiang Xia11Zhiqiang Xia12Changying Zeng13Changying Zeng14Cheng Lu15Cheng Lu16Wenquan Wang17Wenquan Wang18Institute of Tropical Agriculture and Forestry, Hainan UniversityHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaInstitute of Tropical Agriculture and Forestry, Hainan UniversityHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaInstitute of Tropical Agriculture and Forestry, Hainan UniversityHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaThe Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of AgricultureHaikou, ChinaCassava, being one of the top three tuberous crops, features highly efficient starch accumulation in the storage root to adapt the tropical resources and environments. The molecular mechanism for the process, however, is still unclear. ADP-glucose pyrophosphorylase, the first and rate-limited enzyme in starch biosynthesis pathway, is a heterotetramer comprised of two small/catalytic and two large/modulatory subunits. To understand the regulation of MeAGPase, the promoter of a highly expressed small subunit, MeAGPs1a, was used as bait for a yeast one-hybrid assay to screen storage root cDNA library. One cDNA, coding for a small auxin-up RNA protein, named MeSAUR1, was isolated from cassava. MeSAUR1 could bind to the promoter of MeAGPS1a in yeast one-hybrid test and in vitro, and was located in cell nucleus. MeSAUR1 displayed a higher transcript level in cassava root cortex, and its expression was induced by indole-3-acetic acid, gibberellin and ethylene, but repressed by abscisic acid. A dual-luciferase interaction test further convinced that MeSAUR1 could bind to the promoter of MeAGPS1a, and positively regulate the transcription of MeAGPS1a in cassava.http://journal.frontiersin.org/article/10.3389/fpls.2017.01315/fullcassavasmall auxin-up RNA geneADP-glucose pyrophosphorylasestarch biosynthesistranscription factor |
| spellingShingle | Ping’an Ma Ping’an Ma Ping’an Ma Xin Chen Xin Chen Chen Liu Chen Liu Chen Liu Yuhong Meng Yuhong Meng Yuhong Meng Zhiqiang Xia Zhiqiang Xia Changying Zeng Changying Zeng Cheng Lu Cheng Lu Wenquan Wang Wenquan Wang MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava Frontiers in Plant Science cassava small auxin-up RNA gene ADP-glucose pyrophosphorylase starch biosynthesis transcription factor |
| title | MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava |
| title_full | MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava |
| title_fullStr | MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava |
| title_full_unstemmed | MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava |
| title_short | MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava |
| title_sort | mesaur1 encoded by a small auxin up rna gene acts as a transcription regulator to positively regulate adp glucose pyrophosphorylase small subunit1a gene in cassava |
| topic | cassava small auxin-up RNA gene ADP-glucose pyrophosphorylase starch biosynthesis transcription factor |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01315/full |
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