Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry
In recent years, gut-tailored probiotics have been proven to be beneficial for host health. Probiotic strains such as lactic acid bacteria (LAB) are known to exhibit antimicrobial activity, acting as natural substitutes for the regulation of foodborne pathogens. In the present study, a complete anal...
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MDPI AG
2021-10-01
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| author | Hafsa Kanwal Alessandro Di Cerbo Freeha Zulfiqar Carla Sabia Amara Nawaz Fariha Masood Siddiqui Muhammad Aqeel Shakira Ghazanfar |
| author_facet | Hafsa Kanwal Alessandro Di Cerbo Freeha Zulfiqar Carla Sabia Amara Nawaz Fariha Masood Siddiqui Muhammad Aqeel Shakira Ghazanfar |
| author_sort | Hafsa Kanwal |
| collection | DOAJ |
| description | In recent years, gut-tailored probiotics have been proven to be beneficial for host health. Probiotic strains such as lactic acid bacteria (LAB) are known to exhibit antimicrobial activity, acting as natural substitutes for the regulation of foodborne pathogens. In the present study, a complete analysis, isolation, biochemical characterization, and molecular identification of <i>Pediococcus acidilactici</i> (NMCC-11) from Nili Ravi water buffalo (<i>Bubalis bubalis</i>) gut was carried out. NMCC-11 showed the best enzymatic potential, antimicrobial activity against known pathogenic strains, and survivability at a wide pH range (pH 4–pH 6) out of all isolates. The isolates were screened for their antimicrobial activity against the five most infectious microbes such as <i>Escherichia coli</i> (ATCC 8739), <i>Pseudomonas aeruginosa</i> (ATCC9027), <i>Staphylococcus aureus</i> (ATCC6538), <i>Listeria monocytogenes</i> (ATCC13932), and <i>Bacillus cereus</i> (ATCC 11778) using the agar-well diffusion method. Moreover, after NMCC-11 isolation, a comparative diversity analysis against a variety of other randomly selected strains from around the world was carried out using R software. This study showed relatively low genetic diversity, which also contributed to the claim of the stability of this probiotic strain and its potential use as a starter culture and feed probiotic in the dairy industry. However, further studies are certainly warranted to determine its optimal dosage, time frame, and intake frequency. |
| first_indexed | 2024-03-10T06:44:11Z |
| format | Article |
| id | doaj.art-4196968a93644219a9ec0079ab3c7dfc |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T06:44:11Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4196968a93644219a9ec0079ab3c7dfc2023-11-22T17:21:03ZengMDPI AGApplied Sciences2076-34172021-10-011120958610.3390/app11209586Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy IndustryHafsa Kanwal0Alessandro Di Cerbo1Freeha Zulfiqar2Carla Sabia3Amara Nawaz4Fariha Masood Siddiqui5Muhammad Aqeel6Shakira Ghazanfar7Department of Life Sciences, Abasyn University, Islamabad 44000, PakistanSchool of Bioscience and Veterinary Medicine, University of Camerino, 62024 Matelica, ItalyDepartment of Life Sciences, Abasyn University, Islamabad 44000, PakistanDepartment of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Life Sciences, Abasyn University, Islamabad 44000, PakistanDepartment of Life Sciences, Abasyn University, Islamabad 44000, PakistanNational Agricultural Research Centre, National Institute of Genomics and Agriculture Biotechnology (NIGAB), Park Road, Islamabad 45500, PakistanNational Agricultural Research Centre, National Institute of Genomics and Agriculture Biotechnology (NIGAB), Park Road, Islamabad 45500, PakistanIn recent years, gut-tailored probiotics have been proven to be beneficial for host health. Probiotic strains such as lactic acid bacteria (LAB) are known to exhibit antimicrobial activity, acting as natural substitutes for the regulation of foodborne pathogens. In the present study, a complete analysis, isolation, biochemical characterization, and molecular identification of <i>Pediococcus acidilactici</i> (NMCC-11) from Nili Ravi water buffalo (<i>Bubalis bubalis</i>) gut was carried out. NMCC-11 showed the best enzymatic potential, antimicrobial activity against known pathogenic strains, and survivability at a wide pH range (pH 4–pH 6) out of all isolates. The isolates were screened for their antimicrobial activity against the five most infectious microbes such as <i>Escherichia coli</i> (ATCC 8739), <i>Pseudomonas aeruginosa</i> (ATCC9027), <i>Staphylococcus aureus</i> (ATCC6538), <i>Listeria monocytogenes</i> (ATCC13932), and <i>Bacillus cereus</i> (ATCC 11778) using the agar-well diffusion method. Moreover, after NMCC-11 isolation, a comparative diversity analysis against a variety of other randomly selected strains from around the world was carried out using R software. This study showed relatively low genetic diversity, which also contributed to the claim of the stability of this probiotic strain and its potential use as a starter culture and feed probiotic in the dairy industry. However, further studies are certainly warranted to determine its optimal dosage, time frame, and intake frequency.https://www.mdpi.com/2076-3417/11/20/9586<i>Pediococcus acidilactici</i>16S rRNAprobioticpopulation genetic analysisAMOVAMSN |
| spellingShingle | Hafsa Kanwal Alessandro Di Cerbo Freeha Zulfiqar Carla Sabia Amara Nawaz Fariha Masood Siddiqui Muhammad Aqeel Shakira Ghazanfar Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry Applied Sciences <i>Pediococcus acidilactici</i> 16S rRNA probiotic population genetic analysis AMOVA MSN |
| title | Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry |
| title_full | Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry |
| title_fullStr | Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry |
| title_full_unstemmed | Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry |
| title_short | Probiotic Characterization and Population Diversity Analysis of Gut-Associated <i>Pediococcus acidilactici</i> for Its Potential Use in the Dairy Industry |
| title_sort | probiotic characterization and population diversity analysis of gut associated i pediococcus acidilactici i for its potential use in the dairy industry |
| topic | <i>Pediococcus acidilactici</i> 16S rRNA probiotic population genetic analysis AMOVA MSN |
| url | https://www.mdpi.com/2076-3417/11/20/9586 |
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