Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors
Tank bio-oxidation is a biohydrometallurgical technology widely used for metal recovery from sulfide concentrates. Since carbon availability is one of the key factors affecting microbial communities, it may also determine the rate of sulfide concentrate bio-oxidation. The goal of the present work wa...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2350 |
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| author | Aleksandr Bulaev Aleksandra Nechaeva Yuliya Elkina Vitaliy Melamud |
| author_facet | Aleksandr Bulaev Aleksandra Nechaeva Yuliya Elkina Vitaliy Melamud |
| author_sort | Aleksandr Bulaev |
| collection | DOAJ |
| description | Tank bio-oxidation is a biohydrometallurgical technology widely used for metal recovery from sulfide concentrates. Since carbon availability is one of the key factors affecting microbial communities, it may also determine the rate of sulfide concentrate bio-oxidation. The goal of the present work was to evaluate the effect of carbon sources on the bio-oxidation of the concentrate containing 56% pyrite and 14% arsenopyrite at different temperatures (40 and 50 °C) in stirred tank reactors. CO<sub>2</sub> was supplied into the pulp of the first reactor (about 0.01 L/min) and 0.02% (<i>w/v</i>) molasses was added to the pulp of the second one, and no additional carbon sources were used in the control tests. At 40 °C, 77% of pyrite and 98% of arsenopyrite were oxidized in the first reactor, in the second one, 73% of pyrite and 98% of arsenopyrite were oxidized, while in the control reactor, 27% pyrite and 93% arsenopyrite were oxidized. At 50 °C, in the first reactor, 94% of pyrite and 99% of arsenopyrite were oxidized, in the second one, 21% of pyrite and 94% of arsenopyrite were oxidized, while in the control reactor, 10% pyrite and 92% arsenopyrite were oxidized. The analysis of the microbial populations in the reactors revealed differences in the total number of microorganisms and their species composition. Thus, it was shown that the use of various carbon sources made it possible to increase the intensity of the concentrate bio-oxidation, since it affected microbial populations performing the process. |
| first_indexed | 2024-03-10T05:14:37Z |
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| id | doaj.art-419749b9fb8a45fe8b23792e3a6a1f3b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:14:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-419749b9fb8a45fe8b23792e3a6a1f3b2023-11-23T00:30:07ZengMDPI AGMicroorganisms2076-26072021-11-01911235010.3390/microorganisms9112350Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank ReactorsAleksandr Bulaev0Aleksandra Nechaeva1Yuliya Elkina2Vitaliy Melamud3Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33 bld. 2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33 bld. 2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33 bld. 2, 119071 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33 bld. 2, 119071 Moscow, RussiaTank bio-oxidation is a biohydrometallurgical technology widely used for metal recovery from sulfide concentrates. Since carbon availability is one of the key factors affecting microbial communities, it may also determine the rate of sulfide concentrate bio-oxidation. The goal of the present work was to evaluate the effect of carbon sources on the bio-oxidation of the concentrate containing 56% pyrite and 14% arsenopyrite at different temperatures (40 and 50 °C) in stirred tank reactors. CO<sub>2</sub> was supplied into the pulp of the first reactor (about 0.01 L/min) and 0.02% (<i>w/v</i>) molasses was added to the pulp of the second one, and no additional carbon sources were used in the control tests. At 40 °C, 77% of pyrite and 98% of arsenopyrite were oxidized in the first reactor, in the second one, 73% of pyrite and 98% of arsenopyrite were oxidized, while in the control reactor, 27% pyrite and 93% arsenopyrite were oxidized. At 50 °C, in the first reactor, 94% of pyrite and 99% of arsenopyrite were oxidized, in the second one, 21% of pyrite and 94% of arsenopyrite were oxidized, while in the control reactor, 10% pyrite and 92% arsenopyrite were oxidized. The analysis of the microbial populations in the reactors revealed differences in the total number of microorganisms and their species composition. Thus, it was shown that the use of various carbon sources made it possible to increase the intensity of the concentrate bio-oxidation, since it affected microbial populations performing the process.https://www.mdpi.com/2076-2607/9/11/2350biohydrometallurgyacidophilic microorganismspyritearsenopyritesulfide concentratescarbon dioxide |
| spellingShingle | Aleksandr Bulaev Aleksandra Nechaeva Yuliya Elkina Vitaliy Melamud Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors Microorganisms biohydrometallurgy acidophilic microorganisms pyrite arsenopyrite sulfide concentrates carbon dioxide |
| title | Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors |
| title_full | Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors |
| title_fullStr | Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors |
| title_full_unstemmed | Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors |
| title_short | Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors |
| title_sort | effect of carbon sources on pyrite arsenopyrite concentrate bio oxidation and growth of microbial population in stirred tank reactors |
| topic | biohydrometallurgy acidophilic microorganisms pyrite arsenopyrite sulfide concentrates carbon dioxide |
| url | https://www.mdpi.com/2076-2607/9/11/2350 |
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